Biodiversity of antifungal lactic acid bacteria isolated from raw milk samples from cow, ewe and goat over one-year period

Delavenne, Emilie; Mounier, Jérôme; Déniel, F.; Barbier, Georges; Blay, Gwenaëlle Le

doi:10.1016/j.ijfoodmicro.2012.02.003

Cited by 87 publications

(78 citation statements)

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“…As a consequence, the results of the biodiversity analyses do not reflect the entire microbiota, as non-cultivable or strictly anaerobic organisms were not covered. But in contrast to other studies that addressed only a technologically relevant part of the microbiota such as aerobic spore-forming bacteria (Coorevits et al, 2008;De Jonghe et al, 2010) or lactic acid bacteria (Dasen et al, 2003;Delavenne et al, 2012;Pothakos et al, 2014), we tried to analyse a broad cross section of the cultivable microbiota by using TSA and analysing 150 isolates per sample.…”

Section: Discussionmentioning

confidence: 99%

Biodiversity of refrigerated raw milk microbiota and their enzymatic spoilage potential

Neubeck

Baur

Krewinkel

et al. 2015

International Journal of Food Microbiology

185

143

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Section: Discussionmentioning

confidence: 99%

Biodiversity of refrigerated raw milk microbiota and their enzymatic spoilage potential

Neubeck

Baur

Krewinkel

et al. 2015

International Journal of Food Microbiology

185

143

View full text Add to dashboard Cite

“…The number of reports documenting the antifungal activity of LAB has significantly increased during the last decade (Delavenne et al, 2012;Magnusson et al, 2003;Valerio et al, 2009). In addition, information detailing the extensive food-based applications (Baek et al, 2012;Ryan et al, 2008) and identification of the responsible fungal inhibitory compounds (Brosnan et al, 2012;Ström et al, 2002;Yang et al, 2011) has improved.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and morphological profiling of Aspergillus fumigatus Af293 in response to antifungal activity produced by Lactobacillus plantarum 16

et al. 2013

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The morphological effects of an antifungal activity produced by Lactobacillus plantarum 16, a malt-production steep water isolate, on two food-associated fungi were examined microscopically. Spore germination was completely inhibited in Aspergillus fumigatus and Rhizopus stolonifer upon treatment with concentrated cell-free supernatant (cCFS) of strain 16. Furthermore, addition of antifungal cCFS to germ tubes and hyphae halted further development compared to untreated controls. Transcriptome analysis of A. fumigatus Af293 following exposure to antifungal cCFS revealed a number of genes with altered transcription involved in a variety of cellular functions, most notably cell metabolism, suggesting a global metabolic shutdown and subsequent cell death. Increased transcription of the global regulator LaeA was also observed indicating that exposure to the antifungal activity caused a cellular stress response.

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“…The isolation and characterization strategies of bacterial isolates are shown in Figure 1. These 67 isolates were pooled with 20 lactobacilli from a collection of bacteria isolated from raw milks [13]. …”

Section: Resultsmentioning

confidence: 99%

“…Twenty of them belonged to a collection of Lactobacillus spp. (15 L. fermentum , 1 L. brevis and 4 L. parabuchneri ) previously isolated from raw milks in our laboratory [13], and 67 were isolated from the mouth of healthy volunteers during this work (see below). Lactobacillus fermentum ATCC 9338 was obtained from AES Chemunex (Bruz, France), whereas Lactobacillus casei LMG 6904 and Lactobacillus paracasei LMG 13087 were obtained from the BCCM/LMG Bacteria Collection (http://bccm.belspo.be/about/lmg.php).…”

Section: Methodsmentioning

confidence: 99%

Screening of Lactobacillus spp. for the prevention of Pseudomonas aeruginosa pulmonary infections

et al. 2014

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BackgroundPseudomonas aeruginosa is an opportunistic pathogen that significantly increases morbidity and mortality in nosocomial infections and cystic fibrosis patients. Its pathogenicity especially relies on the production of virulence factors or resistances to many antibiotics. Since multiplication of antibiotic resistance can lead to therapeutic impasses, it becomes necessary to develop new tools for fighting P. aeruginosa infections. The use of probiotics is one of the ways currently being explored. Probiotics are microorganisms that exert a positive effect on the host’s health and some of them are known to possess antibacterial activities. Since most of their effects have been shown in the digestive tract, experimental data compatible with the respiratory environment are strongly needed. The main goal of this study was then to test the capacity of lactobacilli to inhibit major virulence factors (elastolytic activity and biofilm formation) associated with P. aeruginosa pathogenicity.ResultsSixty-seven lactobacilli were isolated from the oral cavities of healthy volunteers. These isolates together with 20 lactobacilli isolated from raw milks, were tested for their capacity to decrease biofilm formation and activity of the elastase produced by P. aeruginosa PAO1. Ten isolates, particularly efficient, were accurately identified using a polyphasic approach (API 50 CHL, mass-spectrometry and 16S/rpoA/pheS genes sequencing) and typed by pulsed-field gel electrophoresis (PFGE). The 8 remaining strains belonging to the L. fermentum (6), L. zeae (1) and L. paracasei (1) species were sensitive to all antibiotics tested with the exception of the intrinsic resistance to vancomycin. The strains were all able to grow in artificial saliva.ConclusionEight strains belonging to L. fermentum, L. zeae and L. paracasei species harbouring anti-elastase and anti-biofilm properties are potential probiotics for fighting P. aeruginosa pulmonary infections. However, further studies are needed in order to test their innocuity and their capacity to behave such as an oropharyngeal barrier against Pseudomonas aeruginosa colonisation in vivo.

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Biodiversity of antifungal lactic acid bacteria isolated from raw milk samples from cow, ewe and goat over one-year period

Cited by 87 publications

References 50 publications

Biodiversity of refrigerated raw milk microbiota and their enzymatic spoilage potential

Biodiversity of refrigerated raw milk microbiota and their enzymatic spoilage potential

Transcriptomic and morphological profiling of Aspergillus fumigatus Af293 in response to antifungal activity produced by Lactobacillus plantarum 16

Screening of Lactobacillus spp. for the prevention of Pseudomonas aeruginosa pulmonary infections

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