Biodiversity of a Burkholderia cepacia population isolated from the maize rhizosphere at different plant growth stages

Cello, Francescopaolo Di; Bevivino, Annamaria; Chiarini, Luigi; Fani, Renato; Paffetti, Donatella; Tabacchioni, Silvia; Dalmastri, Claudia

doi:10.1128/aem.63.11.4485-4493.1997

Cited by 259 publications

(117 citation statements)

References 52 publications

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“…Rhizosphere microbial community composition changes as the host plant develops which, in part, is expected to be a response to developmental changes in root-secreted organic compounds (DiCello et al, 1997;Paterson et al, 2007;Houlden et al, 2008). Our results are consistent with these findings, and also highlight other informative features.…”

Section: Dynamic Changes Within Rhizosphere Populationssupporting

confidence: 89%

Temporal dynamics and genetic diversity of chemotactic‐competent microbial populations in the rhizosphere

Buchan¹,

Crombie²,

Alexandre

2010

Environmental Microbiology

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The contribution of chemotaxis to the competitive colonization of the rhizosphere for the vast majority of the soil community is unknown. We have developed and applied a molecular diagnostic tool, based on a gene encoding the central regulator of bacterial chemotaxis (cheA), to characterize and temporally track specific populations of native microbes with chemotaxis potential that are present in soil exposed to two rhizospheres: wheat and cowpea. The data show that the chemotactic-competent communities present in the rhizospheres of the two plants are distinct and less diverse than the bulk soil, indicating the development of unique microbial communities. Consistent with the supposition that selection and recruitment of specific soil microbes takes place in the rhizosphere, the dynamics of specific cheA phylotypes provides support for the hypothesis that chemotaxis provides a competitive advantage to some soil microbes. This is the first study to examine and profile the genetic diversity of chemotaxis genes in natural populations. As such, it illustrates our limited understanding of microbial chemotaxis for the majority of soil microbes. It also highlights the value of a culture-independent approach for examining chemotaxis populations in order to build empirical lines of evidence for its role in structuring of microbial assemblages.

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Section: Dynamic Changes Within Rhizosphere Populationssupporting

confidence: 89%

Temporal dynamics and genetic diversity of chemotactic‐competent microbial populations in the rhizosphere

Buchan¹,

Crombie²,

Alexandre

2010

Environmental Microbiology

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“…The reference strains representing genomovars I±V have all been included in an experimental strain panel described by Mahenthiralingam et al (2000a); B. cepacia genomovar VI and B. ambifaria (genomovar VII) reference strains were chosen from studies described by Coenye et al (2001a, b) ( Table 1). The 120 environmental isolates (Table 2) were obtained from the rhizosphere of maize cultivated in three different fields located in northern (Pieve d'Olmi) (Dalmastri et al, 1999), central (S. Maria di Galeria) (Di Cello et al, 1997) and southern Italy (Dragoni). From the last field, strains were isolated and identified as described by Dalmastri et al (1999).…”

Section: Bacterial Strainsmentioning

confidence: 99%

“…Amplification and restriction analysis of 16S rDNA were performed using procedures described previously (Di Cello et al, 1997). Four of the six enzymes used by Segonds et al (1999) to differentiate Burkholderia species were selected in our study because of their discriminatory power on B. cepacia genomovars, i.e.…”

Section: Amplification and Restriction Analysis Of 16s Rdna (Ardra)mentioning

confidence: 99%

“…B. cepacia can be recovered in high numbers from the maize rhizosphere, in which it represents one of the dominant bacterial species (Hebbar et al, 1994;Di Cello et al, 1997;Dalmastri et al, 1999). Owing to the wide spread of maize cultivation, the maize rhizosphere may represent an important habitat of environmental strains and of potential pathogenic strains.…”

Section: Introductionmentioning

confidence: 99%

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Burkholderia cepacia complex: distribution of genomovars among isolates from the maize rhizosphere in Italy

Fiore¹,

Laevens²,

Bevivino³

et al. 2001

Environmental Microbiology

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Burkholderia cepacia is a 'complex' in which seven genomic species or genomovars have so far been identified. It appears that all seven B. cepacia genomovars are capable of causing infections in vulnerable persons; in particular, the importance of Burkholderia multivorans (genomovar II) and B. cepacia genomovar III among cystic fibrosis isolates, especially epidemic ones, has been emphasized. In order to acquire a better comprehension of the genomovar composition of environmental populations of B. cepacia, 120 strains were isolated from the rhizosphere of maize plants cultivated in fields located in northern, central and southern Italy. The identification of the different genomovars was accomplished by a combination of molecular polymerase chain reaction (PCR)-based techniques, such as restriction fragment length polymorphism (RFLP) analysis of 16S rDNA (ARDRA), genomovar-specific PCR tests and RFLP analyses based on polymorphisms in the recA gene whole-cell protein electrophoresis. ARDRA analysis allowed us to distinguish between all B. cepacia genomovars except B. cepacia genomovar I, B. cepacia genomovar III and Burkholderia ambifaria (genomovar VII). The latter genomovars were differentiated by means of recA PCR tests and RFLP analyses. Among the rhizospheric isolates of B. cepacia, we found only B. cepacia genomovar I, B. cepacia genomovar III, Burkholderia vietnamiensis (genomovar V) and B. ambifaria. B. cepacia genomovars I and III and B. ambifaria were recovered from all three fields, whereas B. vietnamiensis was detected only in the population isolated from the field located in central Italy. Among strains isolated from northern and southern Italy, the most abundant genomovars were B. ambifaria and B. cepacia genomovar III respectively; in contrast, the population isolated in central Italy showed an even distribution of strains among genomovars. These results indicate that it is not possible to differentiate clinical and environmental strains, or pathogenic and non-pathogenic strains, of the B. cepacia complex simply on the basis of genomovar status, and that the environment may serve as a reservoir for B. cepacia genomovar III infections in vulnerable humans.

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“…Among the microorganisms occurring in the rhizo-sphere of maize, Burkholderia cepacia represents probably one of the predominant bacterial species [12]. Previous studies revealed that B. cepacia is present in large numbers associated with the roots and the rhizosphere of maize, both in maize monoculture soils [6] and in a ¢eld with no previous cropping history of maize [13]. Furthermore, B. cepacia has been reported to compete, survive, and colonize roots of various maize cultivars [14,15], to enhance the yield of several crop plants [6,16^19], and to antagonize and repress all the major soilborne fungal pathogens of maize, such as those belonging to the genus Fusarium [20,21].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a free-living maize-rhizosphere population of Burkholderia cepacia: effect of seed treatment on disease suppression and growth promotion of maize

Bevivino

1998

FEMS Microbiology Ecology

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A Burkholderia cepacia population naturally occurring in the rhizosphere of Zea mays was evaluated by metabolic and molecular profiling and for some traits associated with biocontrol and plant growth promoting (PGP) activity. The purpose was to investigate the potential of this bacterial species closely associated with maize to act as a PGP inoculant. The bacterial strains, isolated on semiselective PCAT medium, were assigned to the species B. cepacia by an analysis of the restriction patterns produced by amplified DNA encoding 16S rRNA (16S rDNA) (ARDRA) with the enzyme AluI. Biodiversity among the 14 B. cepacia isolates was analyzed by the Biolog GN system and by the random amplified polymorphic DNA (RAPD) technique with two 10-mer primers. The analysis of the Biolog data revealed that all rhizosphere B. cepacia strains formed a tight phenetic cluster which includes B. cepacia LMG 11351, a reference strain isolated from the maize rhizosphere, and allowed us to distinguish single isolates from one another. The analysis of RAPD patterns allowed us to identify two principal groups in this bacterial population. Other tests included in vitro inhibition of the maize pathogens Fusarium spp., analysis of siderophore production, bioassay using maize seeds coated with B. cepacia in soil artificially infested with the maize pathogens Fusarium spp., and greenhouse-based plant growth promotion experiments with maize. The data obtained demonstrated that all B. cepacia strains displayed a wide antibiosis against the phytopathogenic fungi studied and produced, under low iron conditions, hydroxamate-like and thiazo-like siderophores. Moreover, the bioassay allowed us to select six and eight B. cepacia strains with a potential for the biological control of F. proliferatum ITEM-381 and F. moniliforme ITEM-504, respectively. Growth promotion experiments showed that the effect of seed bacterization with B. cepacia isolates on maize growth depended on the potting medium used. When a sand-peat/manure mixture was used, almost all B. cepacia isolates promoted maize growth ; whereas, when the soil collected from the field of bacterial isolations was used, only four strains exerted a positive effect on maize growth. z

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Biodiversity of a Burkholderia cepacia population isolated from the maize rhizosphere at different plant growth stages

Cited by 259 publications

References 52 publications

Temporal dynamics and genetic diversity of chemotactic‐competent microbial populations in the rhizosphere

Temporal dynamics and genetic diversity of chemotactic‐competent microbial populations in the rhizosphere

Burkholderia cepacia complex: distribution of genomovars among isolates from the maize rhizosphere in Italy

Characterization of a free-living maize-rhizosphere population of Burkholderia cepacia: effect of seed treatment on disease suppression and growth promotion of maize

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