Biodistribution study of radiolabeled monoclonal antibody OC 125 in a nude mouse model intraperitoneally grafted with a human ovarian carcinoma

Thédrez, Philippe; Kremer, M.; Curtet, C.; Sai, Catherine; Guerreau, Dominique; Saccavini, J.C.; Chatal, Jean‐François

doi:10.1016/0883-2897(89)90196-7

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mean tumour-normal (blood, liver, kidney, intestine and spleen) ratios found in the study by Thedrez et al (1989) using "'Inlabelled intact OC 125 injected i.p. into nude mice bearing OVCAR-3 tumours were higher than those found at similar time points with our ['25I]OC125F(ab')2 (at 3 h 6.0 ± 1.4 vs 3.5 ± 0.4 and at 24 h 6.9 ± 2.7 vs 3.2 ± 0.4 respectively).…”

Section: Discussionmentioning

confidence: 99%

Biodistribution of charged F(ab')2 photoimmunoconjugates in a xenograft model of ovarian cancer

Duska

Hamblin²,

Bamberg³

et al. 1997

Br J Cancer

View full text Add to dashboard Cite

Summary The effect of charge modification of photoimmunoconjugates (PICs) on their biodistribution in a xenograft model of ovarian cancer was investigated. Chlorin.6 e6 was attached site specifically to the F(ab')2 fragment of the murine monoclonal antibody OC125, directed against human ovarian cancer cells, via poly-1-lysine linkers carrying cationic or anionic charges. Preservation of immunoreactivity was checked by enzyme-linked immunosorbent assay (ELISA). PICs were radiolabelled with 1251 and compared with non-specific rabbit IgG PICs after intraperitoneal (i.p.) injection into nude mice. Samples were taken from normal organs and tumour at 3 h and 24 h. Tumour to normal 1251 ratios showed that the cationic OC1 25F(ab')2 PIC had the highest tumour selectivity. Ratios for ce6 were uniformly higher than for 1251, indicating that ce6 became separated from 1251. OC1 25F(ab')2 gave highest tissue values of 1251, followed by cationic OC1 25F(ab')2 PIC; other species were much lower. The amounts of cee delivered per gram of tumour were much higher for cationic OC125F(ab')2 PIC than for other species. The results indicate that cationic charge stimulates the endocytosis and lysosomal degradation of the OC1 25F(ab')2-pl-cee that has bound to the i.p. tumour. Positively charged PICs may have applications in the i.p. photoimmunotherapy of minimal residual ovarian cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Biodistribution of charged F(ab')2 photoimmunoconjugates in a xenograft model of ovarian cancer

Duska

Hamblin²,

Bamberg³

et al. 1997

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…OC 125 is a murine MAb of IgG, isotype (Bast et at, 1981) directed against the antigenic determinant CA 125 found in approximately 80% of epithelial ovarian carcinomas (Kabawat et al, 1983). F(ab'), fragments were obtained by pepsin digestion as described by Thedrez et al (1989). An isotypematched MAb, designated TG03, directed against an epitope of the thyroglobulin molecule, was used as non-specific immunoglobulin.…”

Section: Monoclonal Antibodiesmentioning

confidence: 99%

Use of multi‐cell spheroids of ovarian carcinoma as an intraperitoneal radio‐immunotherapy model: Uptake, retention kinetics and dosimetric evaluation

Bardiès

Thédrez

Gestin

et al. 1992

Intl Journal of Cancer

Self Cite

View full text Add to dashboard Cite

The purpose of this study, using multi-cell spheroids as an in vitro model of micrometastases of ovarian carcinoma for i.p. radio-immunotherapy, was to measure the uptake and retention kinetics of 111In-labeled F(ab')2 fragments of OC125 monoclonal antibody (MAb) in spheroids of the NIH:OVCAR-3 cell line and to estimate absorbed doses with beta-emitting radionuclides (kinetics was assumed to be similar to that of indium-111). With 0.2-mm-diameter spheroids at different MAb concentrations the highest binding value was determined. Retention kinetics showed a biological half-life of 50 hr. These data were used to calculate absorbed doses by integration of Berger's latest-point kernels. Mean absorbed doses when spatial distribution was considered to be uniform at the surface (no penetration) or throughout the spheroid (total penetration) were, respectively, 247 and 417 Gy with 153Sm, and 90 and 135 Gy with 90Y. Thus, the use of a similar MAb concentration and specific activity in patients should lead to high absorbed doses in i.p. radio-immunotherapy of micrometastases.

show abstract

Biodistribution study of radiolabeled monoclonal antibody OC 125 in a nude mouse model intraperitoneally grafted with a human ovarian carcinoma

Cited by 2 publications

References 0 publications

Biodistribution of charged F(ab')2 photoimmunoconjugates in a xenograft model of ovarian cancer

Biodistribution of charged F(ab')2 photoimmunoconjugates in a xenograft model of ovarian cancer

Use of multi‐cell spheroids of ovarian carcinoma as an intraperitoneal radio‐immunotherapy model: Uptake, retention kinetics and dosimetric evaluation

Contact Info

Product

Resources

About