Biodistribution of Amine-Amide Chlorin e6 Derivative Conjugate with a Boron Nanoparticle for Boron Neutron-Capture Therapy

Abstract

The aim of the investigation was to study the biodistribution of amino-amide chlorin e 6 derivative conjugate with cobalt bis-dicarbollide as a potential boron transporter for the tasks of boron neutron-capture therapy.Materials and Methods. The experiments were carried out on Balb/c mice with induced murine colon carcinoma CT-26. Amino-amide chlorin e 6 derivative conjugate with cobalt bis-dicarbollide was administered intravenously, the dose being 5 and 10 mg/kg body mass. The sampling for microscopic study of the drug uptake in ex vivo organs and tissues was performed 3 h after the administration.Results. Characteristic nanoconjugate fluorescent peak was found in most organs under study, significant selectivity of the compound being noticed. A high uptake level was recorded in the liver, spleen and lung tissue. At the dose of 5 mg/kg, the drug content in a tumor was not different from that in muscular tissue and skin; maximum uptake was found in the liver. If the dose was increased up to 10 mg/kg, the nanoconjugate content in a tumor appeared to be comparable with that in the liver, the tumor/muscle ratio of fluorescent signals was ~3.Conclusion. The study showed the prospects for using photosensitizer conjugate (chlorin е 6 ) with boron particles as a means to deliver boron into a tumor. The level of the preparation uptake in tumor tissue depends on a dose.

Experimental synthesis, functionalized modifications and potential applications of monoelemental zero-dimensional boron nanomaterials

“…As for synthesized or commercialized 0D boron nanomaterials, suitable modications via surface ligand coupling with other molecules are oen adopted to improve various properties, structures, functions and application performances of products. 77,[129][130][131][132] Guo et al prepared BQDs via rst grinding with a mortar, followed by solvothermal treatment of ground boron powder, NaOH and N-methyl-2-pyrrolidone. 77 The dispersion of BQDs was mixed with PEG-NH 2 .…”

“…Volovetsky et al reported the chemical conjugation action of amine-amide chlorin e6 derivative with a boron nanoparticle. 130 The photosensitizer (chlorin e6) conjugated with boron particles via the amine-amide coupling of chlorin e6 with cobalt bis-dicarbollide (Fig. 10a and b).…”

“…Previous experimental studies have reported luminescence, photoacoustic and photothermal properties of 0D boron nanomaterials, displaying great prospects toward biological imaging applications. 70,76,77,80,81,96,130,131,162 Fluorescence and phosphorescence emission properties of 0D boron nanomaterials have been reported, but very few reports refer to luminescence imaging applications. Rickard et al explored a method for quantifying the oxygen consumption rate of tumor cell lines through using oxygen-sensitive dual-emission BNPs.…”

“…Volovetskiy et al prepared the conjugates of BNPs with amino-amide derivatives of chlorin e 6 containing the linker length (n ¼ 6, 8). 130,131 Under excitation at 514 nm, uorescence signals of the coupled derivatives (not BNPs) were recorded in the range of 560-710 nm. There are observable uorescence distributions (collected in 650-693 nm) in tumor tissues (or muscles, vessels, skin, liver, kidneys, spleen, wall of the small intestine, etc.)…”