Biodistribution and radiation dosimetry of [11C]DASB in baboons

Effects of Citalopram Infusion on the Serotonin Transporter Binding of [11C]DASB in Healthy Controls

“…A similar low parent fraction of the first arterial sample, followed by a higher parent fraction in the second samples has been reported by Parsey et al (2006b). Dynamic acquisitions of the activity distributions in humans, baboons, and rhesus monkeys have shown that for the first 5 mins or so more than 50% of the total injected [ 11 C]DASB activity are concentrated in the lungs Bélanger et al, 2004;Tipre et al, 2004). As the lungs have been described as an organ of high metabolic activity and with a high concentration of serotonin reuptake sites, the intravenous infusion of citalopram before the [ 11 C]DASB administration and the subsequent blockade of the SERT-binding sites in the periphery by citalopram are consistent with the observed increase of the parent fraction at the onset of the PET scan.…”

“…[ 11 C]DASB PET after citalopram infusion in humansphase of the PET scan Bélanger et al, 2004;Tipre et al, 2004). As a consequence of these quite dramatic changes to the time course of the [ 11 C]DASB concentration in the plasma, the kinetics of the [ 11 C]DASB binding in brain tissue was also affected in the sense that the peak activity of the regional TACs occurred earlier during the scanning period than in baseline condition.…”

Empirical bayesian estimation in graphical analysis: A voxel-based approach for the determination of the volume of distribution in PET studies

“…Preparation of the radioligand, emission data acquisition and reconstruction, and determination of arterial input indices were obtained as previously described for [ 11 C]-DASB [13,23]. All emission data were acquired in 3D mode on an ECAT HR+ (Siemens/CTI, Knoxville, TN, U.S.A.) after a 10 minute transmission scan.…”

“…A detailed description of magnetic resonance protocol parameters, de-scalping, and image segmentation between grey matter (GM), white matter, and cerebrospinal fluid voxels has been already published for [ 11 C]-DASB [13,23]. GM voxels are extracted on the basis of GM probability masks (SPM5) with probability threshold of 1%.…”

Impact of Region-of-Interest Delineation Methods, Reconstruction Algorithms, and Intra- and Inter-Operator Variability on Internal Dosimetry Estimates Using PET

“…Several methods for delineating regions-of-interest (ROI) and quantification of dosimetry estimates are currently being used. Whole-organ ROI drawn on PET images or on co-registered high-resolution structural images (computed tomography (CT) or magnetic resonance imaging (MRI)) have been used to estimate the distribution of radioactivity over time [2][3]. However, whole organ ROI delineation is very time-consuming and, as a consequence, simplified methods are also employed.…”