Biodistribution and pharmacokinetics of uniform magnetite nanoparticles chemically modified with polyethylene glycol

Ruiz, Amalia; Hernández, Yulán; Cabal, C.; González, Evelio; Veintemillas-Verdaguer, Sabino; Martínez, Eduardo Justo; Morales, M.P.

doi:10.1039/c3nr01412f

Cited by 97 publications

(102 citation statements)

References 33 publications

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“…The zeta potentials of PAA-coated NPs increased from À50 to À33 mV, and the zeta values of PEI-coated NPs dropped from 51 to 31 mV. The marginal change of À3 to À6 mV for the PEG-coated NPs supported the theory that PEG coatings can prevent non-specific protein absorption (Ruiz et al, 2013). No notable precipitation was observed for all three types of NPs, indicating their stability in cell growth media.…”

Section: Stability Tests Of Polymer-coated Iron Oxide Nanoparticles Isupporting

confidence: 51%

The responses of immune cells to iron oxide nanoparticles

Sherwood

Lackey

et al. 2016

J of Applied Toxicology

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Immune cells play an important role in recognizing and removing foreign objects, such as nanoparticles. Among various parameters, surface coatings of nanoparticles are the first contact with biological system, which critically affect nanoparticle interactions. Here, surface coating effects on nanoparticle cellular uptake, toxicity and ability to trigger immune response were evaluated on a human monocyte cell line using iron oxide nanoparticles. The cells were treated with nanoparticles of three types of coatings (negatively charged polyacrylic acid, positively charged polyethylenimine and neutral polyethylene glycol). The cells were treated at various nanoparticle concentrations (5, 10, 20, 30, 50 μg ml À1 or 2, 4, 8, 12, 20 μg cm À2 ) with 6 h incubation or treated at a nanoparticle concentration of 50 μg ml À1 (20 μg cm À2 ) at different incubation times (6, 12, 24, 48 or 72 h). Cell viability over 80% was observed for all nanoparticle treatment experiments, regardless of surface coatings, nanoparticle concentrations and incubation times. The much lower cell viability for cells treated with free ligands (e.g.~10% for polyethylenimine) suggested that the surface coatings were tightly attached to the nanoparticle surfaces. The immune responses of cells to nanoparticles were evaluated by quantifying the expression of toll-like receptor 2 and tumor necrosis factor-α. The expression of tumor necrosis factor-α and toll-like receptor 2 were not significant in any case of the surface coatings, nanoparticle concentrations and incubation times. These results provide useful information to select nanoparticle surface coatings for biological and biomedical applications.

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Section: Stability Tests Of Polymer-coated Iron Oxide Nanoparticles Isupporting

confidence: 51%

The responses of immune cells to iron oxide nanoparticles

Sherwood

Lackey

et al. 2016

J of Applied Toxicology

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“…This agent may support drug delivery system development, 36 bioprosthetic heart valve construction, 26 preparation of collagen scaffolds, 37 and a large number of other purposes. [38][39][40][41] However, these procedures use EDC to treat biomaterial structures prior to their contact with cells/tissues. The same is not observed when EDC is applied on dentin, since its fluid-filled tubular and porous structure allows EDC to diffuse across dentin to reach the odontoblast-like cells following its application.…”

Section: Discussionmentioning

confidence: 99%

Transdentinal Cytotoxicity of Carbodiimide (EDC) and Glutaraldehyde on Odontoblast-like Cells

Scheffel

Bianchi

Soares

et al. 2015

Operative Dentistry

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Carbodiimide (EDC) and glutaraldehyde are cross-linking agents capable of increasing resin-dentin bond durability. This study showed that these substances might be safely applied on acid-etched dentin since they have no transdentinal cytotoxic effects on cultured odontoblast-like cells. SUMMARYObjective: To evaluate the transdentinal cytotoxicity of three different concentrations of carbodiimide (EDC) or 5% glutaraldehyde (GA) on MDPC-23 cells.Methods: Seventy 0.4-mm-thick dentin disks obtained from human molars were adapted to artificial pulp chambers. MDPC-23 cells were seeded on the pulpal surface of the disks. After 48 hours, the occlusal dentin was acid-etched and treated for 60 seconds with one of the Results: EDC at all test concentrations did not reduce cell viability, while 5% GA did increase cell metabolism. Cell death by necrosis was not elicited by EDC or 5% GA. At the 24-hour period, 0.3 M and 0.5 M EDC reduced TP production by 18% and 36.8%, respectively. At seven days, increased TP production was observed in all groups. Collagen production at the 24-hour period was reduced when 0.5 M EDC was used. After seven days, no difference was observed among the groups. SEM showed no alteration in cell morphology or number, except in the hydrogen peroxide group.Conclusions: Treatment of acid-etched dentin with EDC or GA did not cause transdentinal cytotoxic effects on odontoblast-like cells.

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“…Several polymers, such as polysaccharides, have been used to coat nanoparticles to efficiently increase circulation time (40). Among the various polymers, polyethylene glycol (PEG) is the most widely used strategy to create a steric barrier on the surface of nanoparticles to block the absorption of blood protein (41,42). Poly(ethylene glycol) (PEG) was first introduced in the early 1990s to modify the surface of liposomes to improve pharmacokinetics (PK) after liposomes were intravenously (i.v.)…”

Section: Discussionmentioning

confidence: 99%

Development, Optimization, and Characterization of PEGylated Nanoemulsion of Prostaglandin E1 for Long Circulation

Cheng

Liu

Hu³

et al. 2015

AAPS PharmSciTech

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Abstract. Lipo-PGE1 is the most widely used formulation of PGE1 in clinic. However, PGE1 is easier to leak out from lipo-PGE1 and this will lead to the phlebophlogosis when intravenous injection. The stability of lipo-PGE1 in storage and in vivo is also discounted. The aim of this study is to develop a long-circulating prostaglandin E1-loaded nanoemulsion modified with 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG) to improve the stability and pharmacokinetics profiles of lipo-PGE1. PEGylated PGE1 nanoemulsion was prepared using a dispersing-homogenized method. The stability of nanoemulsion in 1 month was investigated. Pharmacokinetic studies were employed to evaluate the in vivo profile of the optimized nanoemulsion. The optimized nanoemulsion PGE1-PEG2000(1%)-NE showed an oil droplet size <100 nm with a surface charge of −14 mV. Approximately, 97% of the PGE1 was encapsulated in the nanoemulsion. The particle size, zeta potential, and drug loading of PGE1-PEG2000(1%)-NE were stable in 1 month. After PGE1-PEG2000(1%)-NE was intravenously administered to rats, the area under curve (AUC) and half-life of PGE1 were, respectively, 1.47-fold and 5.98-fold higher than those of lipo-PGE1 (commercial formulation). PGE1-PEG2000(1%)-NE was an ideal formulation for prolonging the elimination time of PGE1. This novel parenteral colloidal delivery system of PGE1 has a promising potential in clinic use.

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Biodistribution and pharmacokinetics of uniform magnetite nanoparticles chemically modified with polyethylene glycol

Cited by 97 publications

References 33 publications

The responses of immune cells to iron oxide nanoparticles

The responses of immune cells to iron oxide nanoparticles

Transdentinal Cytotoxicity of Carbodiimide (EDC) and Glutaraldehyde on Odontoblast-like Cells

Development, Optimization, and Characterization of PEGylated Nanoemulsion of Prostaglandin E1 for Long Circulation

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