Biodistribution and general safety of a naked DNA plasmid, GTU®-MultiHIV, in a rat, using a quantitative PCR method

Absolute Quantification of Plasmid DNA by Real-time PCR with Genomic DNA as External Standard and Its Application to a Biodistribution Study of an HIV DNA Vaccine

“…8,19 Other studies found that this inhibition did exist and affected quantification using real-time Standard curves of plasmid pUMVC3-HIV-Gag (ranging from 10 2 to 10 7 copies/ml) were generated in the presence of increasing quantities of gDNA from liver tissue. a. N/A, not available due to undetectability or poor correlation.…”

Co-delivery of ccl19 gene enhances anti-caries DNA vaccine pCIA-P immunogenicity in mice by increasing dendritic cell migration to secondary lymphoid tissues

“…In this study, we provided the direct evidence that CCL19 DNA plasmid entered secondary lymphoid tissues and expressed the chemokine after intramuscular administration. Intramuscular injection is a traditional and widely used method for DNA inoculation, which has been proven to result in plasmid dissemination and protein expression in DLNs as well as at distal sites in lymphoid and nonlymphoid tissues for a couple of days [32][33][34] . However, the mechanisms of plasmid DNA uptake and distribution are still not completely clear.…”

Preclinical and Clinical Safety Studies on DNA Vaccines

“…13,16,17 In rat, plasmid DNA was detected in the lymph nodes. 15 Several weeks after injection, plasmid DNA could only be detected at the site of injection where it persisted for the time of the study. [13][14][15][16][17] Occasionally, plasmid was detected in gonads, but it dissipated rapidly.…”

“…15 Several weeks after injection, plasmid DNA could only be detected at the site of injection where it persisted for the time of the study. [13][14][15][16][17] Occasionally, plasmid was detected in gonads, but it dissipated rapidly. 13,17 The level of plasmid DNA at the injection site was below 100 copies/ µg DNA after initial injection with 100-200 µg DNA.…”

“…Hanke et al 14 studied intramuscular injection of mice with two 50 µg doses of plasmid DNA. Tuomela et al 15 studied the general safety of DNA vaccination in rats who received a single dose of 200 µg plasmid DNA intravenous, intramuscular or intradermal. In all administration methods and at all doses applied, DNA vaccines were well-tolerated and nontoxic.…”