Fish oil production in Indonesia is not only sourced from fish raw materials, but also could be<br />processed by products of canning and fish siege through purification process. The result of purification<br />with one process was not satisfy yet for the parameter oxidation quality fulfillment aspect. Therefore, fish<br />oil by products of fish encircling need to be purified more intensively, such as stratified purification one of<br />them through the process of stratified purification. The aim of this study was to improve the quality of fish<br />oil by-product through stratified purification. The multilevel purification of once purification (P1), twice<br />purification (P2), and three times purification (P3) gives varying results on physical properties, oxidation<br />parameters, and fatty acid profiles. The color of the brightest fish oil was found in sample P2, the lowest<br />density was found in sample P3 (0.63 g /cm3), the highest yield was in P1 sample (44.42%), FFA was lowest<br />in P3 sample (0.37%), The lowest PV in the P1 sample (25.59 meq / kg), the lowest TOTOX in the P1 sample<br />(48.11 meq / kg), and the highest PUFA content in the fatty acid composition found in sample P2 (37.02%).<br />Purified sardine oil extracted from by products of fish stamping by three-stage purification, FFA parameters<br />are able to meet the criteria of IFOS 2014 standards, while for PV, AnV, and TOTOX parameters are able to<br />meet IFOMA standards.<br /><br />