Biodiesel production from Egyptian isolate Fusarium oxysporum NRC2017

Abstract

Background: Biodiesel is a long chain of fatty acid alkyl esters derived from renewable feedstock and it was used as a partial or full substitute for petrol diesel. Results: Among 36 Egyptian filamentous fungi that were screened for their abilities to produce lipid by using Nile red staining assay, Fusarium oxysporum NRC2017 was selected and identified based on the molecular method. Optimization of culture conditions for F. oxysporum NRC2017 maximum lipid production were investigated by using a basal liquid medium with initial pH 5.0, incubation temperature 30°C, and after 9 days of incubation at a static condition. Six types of agro-industrial by-products were used as components of semi-solid-state fermentation, namely breaking rice, rice straw, wheat bran, corn stalk, sweet sorghum, and bagasse. The highest lipid accumulation was obtained when baggase 98.3 mg/g was used. Gas chromatography (GC) analysis of F. oxysporum NRC 2017 biodiesel showed the presence of 2.2% palmitoleic acid, 11.1% oleic acid, 5.6% linoleic acid, 3.0% αlinoleic acid, 2.5% eicosatrienoic acid, 6.8% eicosapentaenoic acid, 16.1% erucic acid, 4.2% margaric acid, and 3.9% behenic acid. Biodiesel from F. oxysporum NRC2017 was blended with petroleum diesel in 5% "B5" and the physical properties results were density 0.84 g/ml, viscosity 2.55 mm 2 /s, cloud point 0°C, iodine value 92.71 gI2/100 g, acid value 1.6 mg/g, and pour point − 1.3°C. Conclusion: This work revealed the possibility of biodiesel production from the Egyptian promising fungal strain F. oxysporum NRC2017.

Enhancement of Biodiesel Production from Fusarium oxysporum NRC 2017 with Various Mutagenesis and Optimization of the Cultural Conditions Using Response Surface Methodology

“…In the previous ndings, they performed the lipid accumulation of F. oxysporum NRC 2017 and the optimal conditions for biomass and lipid content were reached to 49.42%. By utilizing an inoculum size of 1%, adjusting the pH to 5.0, and incubating for 9 days at a 30 o C with using a carbon source (xylose) and a nitrogen source (yeast extract) [15]. In the current work, F. oxysporum NRC 2017 wild type, and the induced mutants were cultivated on the hydrolysate obtained by growing B. cereus 3SME on bagasse as a cheap carbon source.…”

“…F. oxysporum NRC 2017 and B. cereus 3SME strains both have been isolated, identi ed, and deposited with the accession numbers MF62208 and MW522550, respectively, in the Gene Bank [15,16]. F. oxysporum NRC 2017 was produced lipid production and B. cereus 3SME was produced fermentable sugar hydrolysate from bagasse.…”

“…A more production was achievable for the bagasse hydrolysate of fermentable sugar produced using B. cereus 3SME as the sole carbon source [16] and utilizing medium using the modi ed lipid production medium [22]. Based on previous on Sayeda et al, [15], the four critical parameters for lipid production by F. oxysporum NRC 2017were applied. Table (3) presents the results of 29 quadratic Box-Behnken runs utilizing the (A) incubation period, (B) initial pH, (C) incubation temperature, and (D) yeast extract concentration for the F. oxysporum NRC 2017 wild type and its mutants.…”

“…RSM is a recent and advanced technique that yields acceptable ndings for the optimization of ideal conditions for multivariable systems in fermentation processes, replacing the traditional time-and money-consuming methodologies [6]. F. oxysporum NRC 2017 was chosen for producing biodiesel in the previous nding [15]. Using bagasse hydrolyzed by B. cereus 3SM, F. oxysporum NRC 2017 biodiesel production costs will be reduced [16].…”

Response surface methodology for optimization of biodiesel production by the Penicillium commune NRC 2016 and its mutants

“…Among them, biodiesel produced from vegetable oils and animal fats is rather attractive for being biodegradable, clean, nontoxic, and renewable as well as having similar properties to conventional diesel fuels [1]. The lipids produced by oleaginous microorganisms have several bene ts including their high lipid content and rapid growth rate resulting in great production [2][3][4].…”