Biodiesel fuel production from plant oil catalyzed by Rhizopus oryzae lipase in a water-containing system without an organic solvent

Kaieda, Masaru; Samukawa, Taichi; Matsumoto, Takeshi; Ban, Kazuhiro; Kondo, Akihiko; Shimada, Yuji; Noda, Hideo; Nomoto, Fumiki; Ohtsuka, Koutaro; Izumoto, Eiji; Fukuda, Hideki

doi:10.1016/s1389-1723(00)87091-7

Cited by 280 publications

(117 citation statements)

References 13 publications

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“…Lipase transesterification of triglycerides with an alcohol (alcoholysis) involves a two-step mechanism when looking at a single ester bond. The first step is hydrolysis of the ester bond and release of the alcohol moiety followed by an esterification with the second substrate (Kaieda et al, 1999;Miller et al, 1988;Posorske et al, 1988;Xu, 2000). The two steps are represented in Equations (1) and (2) (Paiva et al, 2000)…”

Section: Transesterification With Enzymes Choice Of Enzymesmentioning

confidence: 99%

“…Furthermore, reactor volumes have to be larger to accommodate both solvents and reactants and, except for fossil diesel, the solvents have to be recovered adding extra investment and running costs for distillation equipment. Therefore solvent-free use of enzymes must be the goal for a future industrial production, if the application of enzymes should be environmentally, economically and energetically superior to traditional catalysts (Kaieda et al, 1999). Unfortunately although similar yields have been obtained with and without solvent (Kumari et al, 2007;Soumanou and Bornscheuer, 2003), solvent-free enzymatic biodiesel production is characterized by lower reaction rate than when using solvents (Mittelbach, 1990), and that needs to be improved for the process to be viable.…”

Section: The Combined Influence Of Water Activity and Solvents On Tramentioning

confidence: 99%

“…enzymes (Haas et al, 2006;Kaieda et al, 1999;Komers et al, 2001;Ma and Hanna, 1999;Meher et al, 2006;Suppes et al, 2001Suppes et al, , 2004.…”

Section: Introductionmentioning

confidence: 99%

“…Most often used industrially today is alkaline transesterification (Kaieda et al, 1999;Meher et al, 2006;Srivastava and Prasad, 2000;Zhang et al, 2003), where raw material with a high water or free fatty acid (FFA) content needs pretreatment with an acidic catalyst in order to esterify FFA (Freedman et al, 1984;Kaieda et al, 1999;Zhang et al, 2003), illustrated in Figure 1. Pretreatment is necessary to reduce soap formation during the reaction and ease the extensive handling for separation of biodiesel and glycerol together with removal of catalyst and alkaline wastewater (Meher et al, 2006;Mittelbach, 1990).…”

Section: Introductionmentioning

confidence: 99%

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A review of the current state of biodiesel production using enzymatic transesterification

Fjerbæk

Christensen

Norddahl

2009

Biotech & Bioengineering

662

459

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Enzymatic biodiesel production has been investigated intensively, but is presently employed industrially only in a 20,000 tons/year pilot plant in China (Du et al. [2008] Appl Microbiol Technol 79(3):331-337). This review presents a critical analysis of the current status of research in this area and accentuates the main obstacles to the widespread use of enzymes for commercial biodiesel transesterification. Improved results for enzymatic catalysis are seen with respect to increased yield, reaction time and stability, but the performance and price of the enzymes need further advances for them to become attractive industrially for biodiesel production. Critical aspects such as mass transfer limitations, use of solvents and water activity are discussed together with process considerations and evaluation of possible reactor configurations, if industrial production with enzymes is to be carried out. Results of published studies on the productivity of enzymes are also presented and compared to the use of chemical catalysts.

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Section: Transesterification With Enzymes Choice Of Enzymesmentioning

confidence: 99%

Section: The Combined Influence Of Water Activity and Solvents On Tramentioning

confidence: 99%

“…enzymes (Haas et al, 2006;Kaieda et al, 1999;Komers et al, 2001;Ma and Hanna, 1999;Meher et al, 2006;Suppes et al, 2001Suppes et al, , 2004.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A review of the current state of biodiesel production using enzymatic transesterification

Fjerbæk

Christensen

Norddahl

2009

Biotech & Bioengineering

662

459

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“…os resultados para a obtenção dos ésteres metílicos de soja (72% de rendimento) usando este catalisador a 32 o C durante 72 h, quando a adição do metanol ao meio foi feita em etapas, 182 foram muito similares àqueles descritos com o uso de lipases extracelulares. 183 Transesterificação similar do óleo de soja com metanol na ausên-cia de solvente à temperatura ambiente empregando a R. oryzae imobilizada 184 resultou nos ésteres metílicos com um rendimento melhorado (90%) e uma redução do tempo reacional para 48 h. No caso do óleo de pinhão manso (Jatropha curcas), esse mesmo biocatalisador foi usado na ausência de solvente a 30 o C, durante 60 h, resultando nos ésteres metílicos desejados em 89% de rendimento. 185 Por outro lado, Li et al 186 estudaram a transesterificação do óleo de colza bruto, refinado e também acidificado.…”

Section: Catálise Heterogênea áCida E Alcalinaunclassified

Biodiesel: visão crítica do status atual e perspectivas na academia e na indústria

Dabdoub¹,

Bronzel²,

Rampin³

2009

Quím. Nova

103

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Recebido em 27/2/09; aceito em 31/3/09; publicado na web em 7/4/09 BioDiESEL: A CRiTiCAL oVERViEW oN THE CURRENT STATUS AND PERSPECTiVES AT THE ACADEmY AND iNDUSTRY. This article presents a bibliographic review of research carried out on different alternative processes for biodiesel production. The supercritical and subcritical (non catalytic) reaction conditions, the use of solid basic, solid acid and other heterogeneous catalysts, including the use of immobilized enzymes and whole-cell catalysts are also critically compared with the traditional homogeneous alkaline or acid catalysts that are common on industrial applications. Advantages and limitations of all these processes for the transference from the laboratory to the industry are discussed. A correlation of the chemical composition with the quality parameters of the produced biodiesel is done with aim to stablish adequate procedures for the right selection of the rawmaterial. Castor bean oil is used as an example of inappropriate oil in order to produce a B100 that fulfill all the international physicochemical quality standards. in this article are presented research results to adequate the values of viscosity, density and iodine number of the castor and soybean biodiesel to the international standard limits by means blending these both biodiesels at the right ratio.Keywords: transesterification; biodiesel; catalysts. introduÇãoAumento da demanda mundial por combustíveis líquidos, aquecimento global, segurança energética, vontade política por desenvolvimento nos campos agrícola, social e também energético são pontos que abrem novas áreas de interesse e oportunidades para pesquisas e desenvolvimento na Academia e na indústria, pois são as forças motoras responsáveis pelo renovado interesse na produção de biocombustíveis.Um dos grandes destaques da atualidade na área de biocombustí-veis é o interesse pelo uso de recursos naturais baseados em materiais lignocelulósicos 1-3 para a produção de etanol ou por fontes produtoras de lipídeos usados para a produção de biodiesel.Biodiesel é o nome dado a ésteres alquílicos de ácidos graxos desde que atendam certos parâmetros de qualidade. 4-8 Além de esses ésteres serem derivados de fontes biológicas como plantas e animais, atuam como combustível substituto ao diesel de petróleo, com desempenho muito próximo, não exigindo modificações nos motores. Tradicionalmente o biodiesel é produzido através das reações de transesterificação de triglicerídeos, usando geralmente catalisadores alcalinos, 9 ou da esterificação de ácidos graxos livres, sendo que neste último caso, os ácidos graxos livres (AGLs) são submetidos à reação direta com um álcool de 4 carbonos ou menos, na presença de catalisadores ácidos. 10-12 mais recentemente o uso de diversos catalisadores heterogêneos tem sido descrito na literatura. 13 Transesterificação é o processo de transformação de um éster carboxílico em outro, através da troca do grupo Ro -presente no éster original, por outro grupo semelhante proveniente de um álcool na presença de um catalisa...

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Processes for Production of Biodiesel Fuel

Watanabe

Shimada

2010

Biocatalysis and Biomolecular Engineering

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Biodiesel fuel production from plant oil catalyzed by Rhizopus oryzae lipase in a water-containing system without an organic solvent

Cited by 280 publications

References 13 publications

A review of the current state of biodiesel production using enzymatic transesterification

A review of the current state of biodiesel production using enzymatic transesterification

Biodiesel: visão crítica do status atual e perspectivas na academia e na indústria

Processes for Production of Biodiesel Fuel

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