Biodetection of DNA and proteins using enhanced UV absorption by structuration of the chip surface

Robin, Kristelle; Reverchon, Jean-Luc; Mugherli, Laurent; Fromant, Michel; Bénisty, Henri

doi:10.1117/12.808124

Cited by 2 publications

(3 citation statements)

References 14 publications

(11 reference statements)

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“…The sensitivity optimization of a RWG biochip has been presented in [2]. The materials chosen are silicon nitride (SiN) on silica (SiO 2 ).…”

Section: Contrast Enhancementmentioning

confidence: 99%

“…The basic idea of these biochip is to enhance the interaction between the biological molecules and the wave electric field, and thus to increase the absorption-induced contrast. Multilayer biochips have first been previously [1,2] and showed a sensitivity sufficient to detect an absorption αl in the 10 -3 order (biological monolayer absorption). To be able to detect sub-monolayer molecular binding events in typical biological "spot" sizes (~100 µm), we use coupling properties of light with matter by designing structured biochips.…”

Section: Introductionmentioning

confidence: 99%

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UV imaging of biochips based on resonant grating

et al. 2010

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International audienceIn the frame of biological threat, security systems require label free biochips for rapid detection. Biosensors enable to detect biological interactions, between probes localized at the surface of a chip, and targets present in the sample solution. Here, we present an optical transduction, enabling 2D imaging, and consequently parallel detection of several reactions. It is based on the absorption of biological molecules in the UV domain. Thus, it is based on an intrinsic property of biological molecules and does not require any labelling of the biological molecules. DNA and proteins absorb UV light at 260 and 280 nm respectively. Sensitivity is a major requirement of biosensing devices. Configurations leading to enhancement of the interaction between light and biological molecules are of interest. For a better sensitivity, resonant grating structures are then studied. They enable to confine the electric field close to the biological layer. Imaging of resonant grating is not largely studied, even for visible wavelengths, but it results in good sensitivity. The protein used in this study is the methionyl-tRNA synthetase. Its absorption is representative of protein absorption, and it can then serve as a model for immunological detection. The best experimental contrast due to a monolayer of proteins is 40%. With data processing currently employed for biochip imaging: average on several acquisitions and on all the pixels imaging the biological spots, the device is able to detect a surface density of proteins in the 10 pg/mm range. © (2010) COPYRIGHT SPIE--The International Society for Optical Engineerin

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“…The sensitivity optimization of a RWG biochip has been presented in [2]. The materials chosen are silicon nitride (SiN) on silica (SiO 2 ).…”

Section: Contrast Enhancementmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

UV imaging of biochips based on resonant grating

et al. 2010

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“…The sensitivity optimization of a RWG biochip has been presented in [13]. To fit with mainstream technologies, the materials chosen are silicon nitride (SiN) on silica (SiO 2 ), on a silicon substrate (Si).…”

Section: Resonant Grating Biochip Imagingmentioning

confidence: 99%

2D label-free imaging of resonant grating biochips in ultraviolet

Bougot-Robin¹,

Reverchon²,

Fromant³

et al. 2010

Opt. Express

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2D images of label-free biochips exploiting resonant waveguide grating (RWG) are presented. They indicate sensitivities on the order of 1 pg/mm 2 for proteins in air, and hence 10 pg/mm 2 in water can be safely expected. A 320×256 pixels Aluminum-Gallium-Nitride-based sensor array is used, with an intrinsic narrow spectral window centered at 280 nm. The additional role of characteristic biological layer absorption at this wavelength is calculated, and regimes revealing its impact are discussed. Experimentally, the resonance of a chip coated with protein is revealed and the sensitivity evaluated through angular spectroscopy and imaging. In addition to a sensitivity similar to surface plasmon resonance (SPR), the RWGs resonance can be flexibly tailored to gain spatial, biochemical, or spectral sensitivity. S. Unlü, "Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications," Proc. Natl. Acad. Sci. U.S.A. 105(23), 7988-7992 (2008). ©2010 Optical Society of America

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Biodetection of DNA and proteins using enhanced UV absorption by structuration of the chip surface

Abstract: International audienc

Cited by 2 publications

References 14 publications

UV imaging of biochips based on resonant grating

UV imaging of biochips based on resonant grating

2D label-free imaging of resonant grating biochips in ultraviolet

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