Biodesulfurization of dibenzothiophene by recombinant Gordonia alkanivorans RIPI90A

Shavandi, Mahmoud; Sadeghizadeh, Majid; Zomorodipour, Alireza; Khajeh, Khosro

doi:10.1016/j.biortech.2008.06.011

Cited by 50 publications

(24 citation statements)

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“…Moreover, decay of the BDS activity due to the accumulation of the sulfur-free end product 2HBP in bacterial biocatalysts has been reported (Abin-Fuentes et al, 2013). It has been shown that bacteria harboring identical dsz genes provide different BDS rates due to the influence of the host metabolism (Kilbane, 2006 (Kilbane, 2006;Li et al, 2008;Ma et al, 2006;Shavandi et al, 2009; Wang et al, 2011). Despite all these successful trials to produce genetically improved biocatalysts, so far, none of the deployed gene manipulation techniques has resulted in a BDS rate meeting the industrial requirement for the development of a commercial BDS process (Boniek et al, 2015;Kilbane, 2006).…”

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confidence: 99%

Engineering synthetic bacterial consortia for enhanced desulfurization and revalorization of oil sulfur compounds

Martínez

Mohamed

Rozas

et al. 2016

Metabolic Engineering

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The 4S pathway is the most studied bioprocess for the removal of the recalcitrant sulfur of aromatic heterocycles present in fuels. It consists of three sequential functional units, encoded by the dszABCD genes, through which the model compound dibenzothiophene (DBT) is transformed into the sulfur-free 2-hydroxybiphenyl (2HBP) molecule. In this work, a set of synthetic dsz cassettes were implanted in Pseudomonas putida KT2440, a model bacterial "chassis" for metabolic engineering studies. The complete dszB1A1C1-D1 cassette behaved as an attractive alternative -to the previously constructed recombinant dsz cassettes -for the conversion of DBT into 2HBP. Refactoring the 4S pathway by the use of synthetic dsz modules encoding individual 4S pathway reactions revealed unanticipated traits, e.g., the 4S intermediate 2HBP-sulfinate (HBPS) behaves as an inhibitor of the Dsz monooxygenases, and oncesecreted from the cells it cannot be further taken up. That issue should be addressed for the rational design of more efficient biocatalysts for DBT bioconversions. In this sense, the construction of synthetic bacterial consortia to compartmentalize the 4S pathway into different cell factories for individual optimization was shown to enhance the conversion of DBT into 2HBP, overcome the inhibition of the Dsz enzymes by the 4S intermediates, and enable efficient production of unattainable high added value intermediates, e.g., HBPS, that are difficult to obtain using the current monocultures. IntroductionCrude oils contain undesirable contaminant molecules, such as thiophenic aromatics compounds, which have a negative impact on oil processing and pose serious environmental threats (Soleimani et al., 2007). A wide spectrum of desulfurization technologies have been developed to remove sulfur mainly from finished refinery products (Stanislaus et al., 2010). Hydrodesulfurization (HDS) treatment has proved to be the common technology of choice to reduce the level of sulfur in crude oil products.Significant environmental, technical and economic limitations have been reported in applying the HDS process (Babich and Moulijn, 2003).During the past 30 years, research to develop alternative desulfurization technologies resulted in a biotechnological strategy to eliminate sulfur from thiophenic compounds (biodesulfurization (BDS)) via serial reactions known as the 4S pathway (Gray et al., 2003;Gupta et al., 2005;Kilbane, 2006;Monticello, 2000;Nuhu, 2013; Xu et al., 2009). This pathway was firstly reported in the gram-positive bacteriumRhodococcus erythropolis IGTS8 (Gallagher et al., 1993), but the 4S pathway has been also found in other bacteria (Duarte et al., 2001;Kilbane, 2006;Mohebali and Ball, 2008).The 4S pathway provides a nondestructive oxidative process used by the cells to obtain the sulfur required for growth, which involves the transformation of dibenzothiophene (DBT), the model compound for sulfur heterocycles present in oil and refractory to HDS, into 2-hydroxybiphenyl (2HBP) and sulfite ( Fig. 1A) (Gallagher et al...

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confidence: 99%

Engineering synthetic bacterial consortia for enhanced desulfurization and revalorization of oil sulfur compounds

Martínez

Mohamed

Rozas

et al. 2016

Metabolic Engineering

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“…The results of GC studies revealed that 2-HBP accumulated as a metabolite of DBT desulfurization. Moreover, Park et al (2003) also described recombinant K. oxytoca transformed 0.7-2.4% of the DBT (Jeong et al, 1998b), demonstrating that improvement in biocatalyst properties and higher desulfurization activity have been achieved by promoter replacement (Shavandi et al, 2009). On the other hand, it has been reported that dszD encoding flavin reductase is crucial for three oxidations in the 4S pathway and subsequently affects the desulfurization activity (Ohshiro et al, 2002;Soleimani et al, 2007).…”

Section: Desulfurization Assaymentioning

confidence: 95%

Designing a New Recombinant IndigenousKlebsiella oxytocaISA4 by Cloning ofdszGenes

Aliebrahimi

Raheb

Ebrahimipour

et al. 2015

Energy Sources, Part A: Recovery, Utilization, and Environmenta

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A bacterium designated as a Klebsiella oxytoca ISA4 was isolated from oil-contaminated soil; it not only is unable to catabolize dibenzothiophene as a sole source of carbon and energy but also produces biosurfactant. In order to enhance the efficiency of biodesulfurization, the dszABC genes from Rhodococcus erythropolis IGTS8 were amplified by specific primers. PCR ampilicon of 3.8 kb was obtained and cloned using pVLT31 vector in native K. oxytoca ISA4. Further analysis by Gibbs assay and gas chromatograph revealed that recombinant K. oxytoca ISA4 has the highest desulfurization ability (48%) in comparison with R. erythropolis IGTS8 (42%) and Pseudomonas aeruginosa pTSOX4 (46%).

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“…RIPI90A dsz ABC genes inserted into the E. coli pRSG43 plasmid when present in G. alkalivorans were expressed with the help of the lac operon. The transformed cells having a transformation efficiency of 1.65 × 10 5 CFUs μg − 1 plasmid DNA exhibited a 2.67-fold increase in desulphurization activity when compared to wild type cells, even in the presence of inorganic sulphate [107]. Still other studies showed the plasmid pUC19 to be a carrier and vector for the desulphurization genes of G. alkalivorans 1B [108].…”

Section: Genetic Modificationmentioning

confidence: 95%

Biodegradation of dibenzothiophene and its application in the production of clean coal

Mishra

Pradhan

Panda

et al. 2016

Fuel Processing Technology

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Biodesulfurization of dibenzothiophene by recombinant Gordonia alkanivorans RIPI90A

Cited by 50 publications

References 17 publications

Engineering synthetic bacterial consortia for enhanced desulfurization and revalorization of oil sulfur compounds

Engineering synthetic bacterial consortia for enhanced desulfurization and revalorization of oil sulfur compounds

Designing a New Recombinant IndigenousKlebsiella oxytocaISA4 by Cloning ofdszGenes

Biodegradation of dibenzothiophene and its application in the production of clean coal

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