Biodegradation of triclosan by a wastewater microorganism

Lee, Do Gyun; Zhao, Fuman; Rezenom, Yohannes H.; Russell, David H.; Chu, Kung-Hui

doi:10.1016/j.watres.2012.05.025

Cited by 152 publications

(78 citation statements)

References 38 publications

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“…There are very few isolated PPCP degraders in the literature, but one of the few is strain KCY1 (DQ983313), which was isolated from activated sludge and has been demonstrated to dechlorinate triclosan (47). Four sequences detected in our column and SAT samples at relative abundances as high as 0.071% had Ն97% similarity with strain KCY1 (see Table S9 in the supplemental material).…”

Section: Discussionmentioning

confidence: 98%

Removal of Pharmaceuticals and Personal Care Products during Water Recycling: Microbial Community Structure and Effects of Substrate Concentration

Onesios-Barry

Berry

Proescher

et al. 2014

Appl Environ Microbiol

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Many pharmaceuticals and personal care products (PPCPs) have been shown to be biotransformed in water treatment systems. However, little research exists on the effect of initial PPCP concentration on PPCP biotransformation or on the microbial communities treating impacted water. In this study, biological PPCP removal at various concentrations was assessed using laboratory columns inoculated with wastewater treatment plant effluent. Pyrosequencing was used to examine microbial communities in the columns and in soil from a soil aquifer treatment (SAT; a method of water treatment prior to reuse) site. Laboratory columns were supplied with different concentrations (0.25, 10, 100, or 1,000 g liter ؊1 ) of each of 15 PPCPs. Five PPCPs (4-isopropyl-3-methylphenol [biosol], p-chloro-m-xylenol, gemfibrozil, ketoprofen, and phenytoin) were not removed at any tested concentrations. Two PPCPs (naproxen and triclosan) exhibited removals independent of PPCP concentration. PPCP removal efficiencies were dependent on initial concentrations for biphenylol, p-chloro-m-cresol, chlorophene, diclofenac, 5-fluorouracil, ibuprofen, and valproic acid, showing that PPCP concentration can affect biotransformation. Biofilms from sand samples collected from the 0.25-and 10-g liter ؊1 PPCP columns were pyrosequenced along with SAT soil samples collected on three consecutive days of a wetting and drying cycle to enable comparison of these two communities exposed to PPCPs. SAT communities were similar to column communities in taxonomy and phylotype composition, and both were found to contain close relatives of known PPCP degraders. The efficiency of biological removal of PPCPs was found to be dependent on the concentration at which the contamination occurs for some, but not all, PPCPs.

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Section: Discussionmentioning

confidence: 98%

Removal of Pharmaceuticals and Personal Care Products during Water Recycling: Microbial Community Structure and Effects of Substrate Concentration

Onesios-Barry

Berry

Proescher

et al. 2014

Appl Environ Microbiol

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“…4 Therefore, different research groups have been focusing on other degradation processes, such as photochemical transformations and photocatalytic decompositions in order to increase removal efficiency. 10 Recently, a lot of research has been focused on the area of microbial biodegradation or biotransformation of antibiotics [11][12][13] , because biological treatment is a preferred option in terms of economic costs and ecological footprint. The ability of microorganisms to metabolize complex chemicals and large amounts of organic compounds represent one of the most important processes.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Erythromycin with Environmental Microorganism Pseudomonas aeruginosa 3011

Šabić

Čižmek

Domanovac

et al. 2015

Chem. Biochem. Eng. Q.

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“…This pathway is distinct from the catabolic pathway of triclosan proposed for Sphingopyxis sp. KCY (Lee et al 2012). Although both pathways begin with formation of hydroxytriclosan, the proposed pathway for KCY does not involve cleavage of the bis-aryl ether bond in hydroxytriclosan.…”

Section: Discussionmentioning

confidence: 95%

“…PH-07 (Kim et al 2011), Sphingopyxis sp. KCY (Lee et al 2012), Mycobacterium vaccae JOB5 and Rhodococcus jostii RHA1 , have also been reported to catabolize triclosan. Methylobacillus was implicated as a triclosan assimilating organism in one stable isotope study of a sewage sludge-derived enrichment culture (Lolas et al 2012) while uncultured AlicycliphilusḐ efluvibacter and Stenotrophomonas were implicated in another .…”

Section: Introductionmentioning

confidence: 99%

Identification of a gene cluster associated with triclosan catabolism

2015

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Aerobic degradation of bis-aryl ethers like the antimicrobial triclosan typically proceeds through oxygenase-dependent catabolic pathways. Although several studies have reported on bacteria capable of degrading triclosan aerobically, there are no reports describing the genes responsible for this process. In this study, a gene encoding the large subunit of a putative triclosan oxygenase, designated tcsA was identified in a triclosan-degrading fosmid clone from a DNA library of Sphingomonas sp. RD1. Consistent with tcsA's similarity to two-part dioxygenases, a putative FMN-dependent ferredoxin reductase, designated tcsB was found immediately downstream of tcsA. Both tcsAB were found in the midst of a putative chlorocatechol degradation operon. We show that RD1 produces hydroxytriclosan and chlorocatechols during triclosan degradation and that tcsA is induced by triclosan. This is the first study to report on the genetics of triclosan degradation.

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Biodegradation of triclosan by a wastewater microorganism

Cited by 152 publications

References 38 publications

Removal of Pharmaceuticals and Personal Care Products during Water Recycling: Microbial Community Structure and Effects of Substrate Concentration

Removal of Pharmaceuticals and Personal Care Products during Water Recycling: Microbial Community Structure and Effects of Substrate Concentration

Biodegradation of Erythromycin with Environmental Microorganism Pseudomonas aeruginosa 3011

Identification of a gene cluster associated with triclosan catabolism

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