Biodegradation of the sulfonylurea herbicide chlorimuron-ethyl by the strain<i>Pseudomonas</i>sp. LW3

Ma, Jie; Wang, Zhe; Lu, Peng; Hui-jie, Wang; Ali, Syed Shahid; Li, Shunpeng; Huang, Xing

doi:10.1111/j.1574-6968.2009.01638.x

Cited by 81 publications

(47 citation statements)

References 32 publications

(35 reference statements)

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“…Pseudomonas sp. LW3 could degrade bensulfuron-methyl, thifensulfuron-methyl, ethametsulfuron-methyl, pyrazosulfuron-ethyl, nicosulfuron and triflusulfuronmethyl, but not degrade metsulfuron-methyl (Ma et al 2009). In the present study, the degradation of metsulfuron-methyl by strain ZWS16 was faster than that of Pseudomonas sp.…”

Section: Degradation Of Other Sulfonylurea Herbicidesmentioning

confidence: 97%

Biodegradation of thifensulfuron-methyl by Ochrobactrum sp. in liquid medium and soil

Zhao

et al. 2015

Biotechnol Lett

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Section: Degradation Of Other Sulfonylurea Herbicidesmentioning

confidence: 97%

Biodegradation of thifensulfuron-methyl by Ochrobactrum sp. in liquid medium and soil

Zhao

et al. 2015

Biotechnol Lett

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“…To date, several microorganisms capable of degrading sulfonylurea herbicides have been described (5,17,21,22,27,32,45,46). The reported biodegradation pathways of sulfonylurea herbicides include oxidation, de-esterification, and cleavage of the sulfonylurea bridge (27,28,32,45,46). Two sulfonylurea oxidation cytochrome P450 monooxygenases, P450SU1 and P450SU2, were identified in Streptomyces griseolus ATCC 11796 (31, 32); their coding genes, suaC and subC, have been cloned and characterized (34).…”

mentioning

confidence: 99%

SulE, a Sulfonylurea Herbicide De-Esterification Esterase from Hansschlegelia zhihuaiae S113

Hang

Hong

Xie

et al. 2012

Appl Environ Microbiol

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De-esterification is an important degradation or detoxification mechanism of sulfonylurea herbicide in microbes and plants. However, the biochemical and molecular mechanisms of sulfonylurea herbicide de-esterification are still unknown. In this study, a novel esterase gene, sulE, responsible for sulfonylurea herbicide de-esterification, was cloned from Hansschlegelia zhihuaiae S113. The gene contained an open reading frame of 1,194 bp, and a putative signal peptide at the N terminal was identified with a predicted cleavage site between Ala37 and Glu38, resulting in a 361-residue mature protein. SulE minus the signal peptide was synthesized in Escherichia coli BL21 and purified to homogeneity. SulE catalyzed the de-esterification of a variety of sulfonylurea herbicides that gave rise to the corresponding herbicidally inactive parent acid and exhibited the highest catalytic efficiency toward thifensulfuron-methyl. SulE was a dimer without the requirement of a cofactor. The activity of the enzyme was completely inhibited by Ag ؉ , Cd 2؉ , Zn 2؉ , methamidophos, and sodium dodecyl sulfate. A sulE-disrupted mutant strain, ⌬sulE, was constructed by insertion mutation. ⌬sulE lost the de-esterification ability and was more sensitive to the herbicides than the wild type of strain S113, suggesting that sulE played a vital role in the sulfonylurea herbicide resistance of the strain. The transfer of sulE into Saccharomyces cerevisiae BY4741 conferred on it the ability to de-esterify sulfonylurea herbicides and increased its resistance to the herbicides. This study has provided an excellent candidate for the mechanistic study of sulfonylurea herbicide metabolism and detoxification through de-esterification, construction of sulfonylurea herbicide-resistant transgenic crops, and bioremediation of sulfonylurea herbicide-contaminated environments. S ulfonylurea herbicides are an important class of herbicides used worldwide for controlling weeds in all major agronomic crops. The herbicides inhibit acetohydroxy acid synthase (AHAS), a key enzyme in the biosynthesis pathway of branched-chain amino acids valine, leucine, and isoleucine in bacteria, fungi, and plants (3,4,8,13). The use of sulfonylurea herbicides has developed rapidly because of their high efficacies at low dosages and multicrop selectivities. The sulfonylurea products are now the second most common kind of herbicides after the glyphosates, and more than 30 products have been commercialized.Most sulfonylurea herbicides are weak acids, vulnerable to acid hydrolysis under acidic conditions. However, in neutral to alkaline soils, some of the herbicides, such as metsulfuron-methyl, chlorsulfuron, and ethametsulfuron-methyl, are degraded at a very slow rate and persist from several months to more than 1 year (15,20,24,30). The residues of herbicides in the soil seriously damage subsequent rotation of sulfonylurea-sensitive crops, like legumes and oilseeds, which can result in serious agricultural loss. Thus, great concern and interest have been raised regarding the enviro...

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“…Based on the morphological and biochemical characterization of the isolates, we identified the cultures as belonging to the versatile bacterial genus Pseudomonas. Previous reports have suggested that this genus can degrade a number of chemicals and pesticides, including oxadiazon (Garbi et al, 2006), sulfonylurea (Ma et al, 2009), p-nitrophenol (Zheng et al, 2009) and phenolic compounds (Li et al, 2010). Thus, this genus has immense nutritional diversity.…”

Section: Discussionmentioning

confidence: 99%

“…Wild-type strain AF7 was grown in King's B medium (Ma et al, 2009) in 5-mL cultures at 28°C to an OD 600 . The bacterial cells were then pelleted by centrifugation (3000 g, 10 min, 4°C), washed three times with ice-cold distilled water, and resuspended in 500 μL ice-cold glycerol.…”

Section: Fluorescent Labeling Of Pseudomonas Strain Af7mentioning

confidence: 99%

Diversity of propanil-degrading bacteria isolated from rice rhizosphere and their potential for plant growth promotion

Procópio¹,

Procopio²,

Pizzirani-Kleiner³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. The herbicide propanil has long been used in rice production in southern Brazil. Bacteria isolated from contaminated soils in Massaranduba, Santa Catarina, Brazil, were found to be able to grow in the presence of propanil, using this compound as a carbon source. Thirty strains were identified as Pseudomonas (86.7%), Serratia (10.0%), and Acinetobacter (3.3%), based on phylogenetic analysis of 16S rDNA. Little genetic diversity was found within species, more than 95% homology, suggesting that there is selective pressure to metabolize propanil in the microbial community. Two strains of Pseudomonas (AF7 and AF1) were selected in bioreactor containing chemotactic growth medium, with the highest degradation activity of propanil exhibited by strain AF7, followed by AF1 (60 and 40%, respectively). These strains when encapsulated in alginate exhibited a high survival rate and were able to colonize the rice root surfaces. Inoculation with Pseudomonas strains AF7 and AF1 significantly improved the plant height of rice. Most of the Pseudomonas strains produced indoleacetic acid, soluble mineral phosphate, and fixed nitrogen. These bacterial strains could potentially be used for the bioremediation of propanil-contaminated soils and the promotion of plant growth.

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Biodegradation of the sulfonylurea herbicide chlorimuron-ethyl by the strainPseudomonassp. LW3

Cited by 81 publications

References 32 publications

Biodegradation of thifensulfuron-methyl by Ochrobactrum sp. in liquid medium and soil

Biodegradation of thifensulfuron-methyl by Ochrobactrum sp. in liquid medium and soil

SulE, a Sulfonylurea Herbicide De-Esterification Esterase from Hansschlegelia zhihuaiae S113

Diversity of propanil-degrading bacteria isolated from rice rhizosphere and their potential for plant growth promotion

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