Biodegradation of the herbicide trifluralin by bacteria isolated from soil

Bellinaso, Maria de Lourdes

doi:10.1016/s0168-6496(02)00388-4

Cited by 9 publications

(14 citation statements)

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“…On the other hand, permanent tolerance (resistance) occurs due to genetic modifications, inherited by the subsequent generation of microbes. The rate of genetic variations is particularly high in microbes and the frequency of evolved resistance depends on the gene mutation frequency (Bellinaso et al 2003, Johnsen et al 2001.…”

Section: Isolation and Molecular Identificationmentioning

confidence: 99%

Plant growth promoting activities of phosphate-solubilizing Enterobacter asburiae as influenced by fungicides

Ahemad¹,

Khan²

2010

EJOBIOS

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Four fungicides, tebuconazole, hexaconazole, metalaxyl and kitazin were applied at recommended and higher rates to examine their effects on the plant growth promoting activities of the fungicide tolerant Enterobacter asburiae strain PS2 under in vitro conditions. Enterobacter asburiae strain PS2 was isolated from the mustard rhizosphere and was assessed for the fungicide-tolerance and production of plant growth promoting traits (phosphate solubilization, siderophores, indole acetic acid, exo-polysaccharides, hydrogen cyanide and ammonia production) both in the presence and absence of fungicides. Enterobacter asburiae strain PS2 showed plant growth promoting activities even in the presence of fungicides which however, decreased progressively with the increase in fungicide concentration. Fungicides at recommended dose had little effect while the dose higher than the recommended one adversely affected the physiological traits, like, phosphate solubilization, siderophore, and indole acetic acid synthesis. Among all fungicides, tebuconazole in general, showed maximum toxicity to plant growth promoting activities. Fungicide-tolerant E. asburiae strain PS2 with the inherent ability to produce growth regulators even in the presence of fungicides can be exploited as a bio-inoculant to increase the productivity of crops grown in fungicide stressed soils. Keywords: Enterobacter, fungicide, phosphate solubilization, plant growth promoting rhizobacteria (PGPR). Ahemad M, Khan MS (2010) Plant growth promoting activities of phosphate-solubilizing Enterobacter asburiae as influenced by fungicides. EurAsia J BioSci 4: 88-95.

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Section: Isolation and Molecular Identificationmentioning

confidence: 99%

Plant growth promoting activities of phosphate-solubilizing Enterobacter asburiae as influenced by fungicides

Ahemad¹,

Khan²

2010

EJOBIOS

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“…This herbicide is moderately persistent in soil and is widely used in controlling a wide variety of annual grasses and broadleaf weeds in many agronomic and horticultural crops. [4][5][6][7][8][9] Golab et al, [4] in an extensive field study using radiolabelled trifluralin, found that after one year, 69% of the applied 14 C was present in the top 0-15 cm of soil: 14% as trifluralin, 12% as extractable transformation products, and 43% as nonextractable soil-bound residues. [3] A number of workers have investigated persistence, degradation, and movement of trifluralin in soil and plants.…”

Section: Introductionmentioning

confidence: 99%

“…In a soil used for the disposal of various pesticides (diuron, trifluralin, carbofuran), only trifluralin was still detected after many years. [9] The microbial activity of soil has an important role on the degradation of dinitroanilines. [9] The microbial activity of soil has an important role on the degradation of dinitroanilines.…”

Section: Introductionmentioning

confidence: 99%

“…[10] It was proven in another recent study [11,12] that a species of Pseudomonas was responsible for decomposing trifluralin. Some researchers [9] worked on biodegradation of the herbicide trifluralin by bacteria isolated from soil and reported that the five positive isolates can be used to study the biochemical and molecular biology of trifluralin biodegradation with the aim of optimising the degradative ability of one or more of the isolates for future use in bioremediation processes. Zimhdahl and Gwynn [12] found that trifluralin transformed to 3,4,5 triamine by the aerobic and anaerobic degradation.…”

Section: Introductionmentioning

confidence: 99%

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Biodegradation of Trifluralin in Harran Soil

Tiryaki¹,

Y�cel²,

Sezen³

2004

J Environ Sci Health B Pest Food Contam Agricul Wastes

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Degradation of trifluralin (alpha, alpha, alpha-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine) was investigated in soils taken from three different locations at Harran region of Turkey under laboratory conditions. Surface (0-10 cm) soils, which were taken from a pesticide untreated field Gürgelen, Harran-1 and Ikizce regions in the Harran Plain. were incubated in biometer flasks for 350 days at 25 degrees C. Ring-UL-14C-trifluralin was applied at the rate of 2 microg g(-1) with 78.7 kBq radioactivity per 100 g soil flask. Evolved (14)CO2 was monitored in KOH traps throughout the experiment. Periodically, soil sub-samples were removed and extracted by supercritical fluid extraction (SFE). Unextractable soil-bound 14C residues were determined by combustion. During the 350 days incubation period 6.6, 5.4, and 3.3/' of the applied radiocarbon was evolved as (14)CO2 from the Harran-1, Gürgelen, and Ikizce soil, respectively. At the end of 350 days the SFE-extractable and bound 14C-trifluralin residues were 39.0 and 29.2% of the initially applied herbicide in Gürgelen soil. The corresponding values for Harran-1 and Ikizce soils were 36.2, 28.4% and 41.6, 18.5% respectively.

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“…Trifluralin (α,α,α‐trifluoro‐2,6‐dinitro‐ N,N ‐dipropyl‐ p ‐toluidine) is a dinitroaniline compound which was first produced in the 1960s and has been used extensively as an agricultural herbicide. There is little information on the biodegradation of this compound by pure bacterial isolates8–11 and to our knowledge no work has been done on the genetic aspects of the catabolism of this molecule. In this article, we report the analysis of DNA isolated from bacteria shown to degrade trifluralin, using as probes the catabolic genes ndoB, todC1 and xylX , which encode the enzymes naphthalene 1,2‐dioxygenase,12 toluene dioxygenase13 and toluate 1,2‐dioxygenase14 respectively (enzymes that prepare the aromatic ring for the cleavage step), in addition to the genes catA and xylE , which encode catechol 1,2‐dioxygenase15 and catechol 2,3‐dioxygenase16 (the ring‐cleaving enzymes), respectively (Fig 1).…”

Section: Introductionmentioning

confidence: 99%

Genes similar to naphthalene dioxygenase genes in trifluralin‐degrading bacteria

Bellinaso

Henriques

Gaylarde

et al. 2003

Pest Management Science

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Trifluralin (alpha,alpha,alpha-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine) is a dinitroaniline compound which was first produced in the 1960s and has been used extensively as an agricultural herbicide. There are a few publications on the biodegradation of this xenobiotic compound, but to our knowledge nothing has been documented on the genetic aspects of its catabolism. In this article, we report the analysis of DNA isolated from bacteria previously shown to degrade trifluralin, using as probes the catabolic genes ndoB, todC, xyIX, catA and xyIE which encode the enzymes naphthalene 1,2-dioxygenase, toluene dioxygenase, toluate 1,2-dioxygenase, catechol 1,2-dioxygenase and catechol 2,3-dioxygenase respectively. Using PCR and hybridization analysis, the strong hybridization of the ndoB gene with DNA extracted from four trifluralin-degrading isolates was demonstrated, although none of them was able to degrade naphthalene, as indicated by the 'clear zone' test. The results indicated the presence in these bacteria of a dioxygenase gene, whose product could act on trifluralin as its principal substrate, or fortuitously, by cometabolism. This is the first publication on genes in trifluralin-degrading bacteria.

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Biodegradation of the herbicide trifluralin by bacteria isolated from soil

Cited by 9 publications

References 0 publications

Plant growth promoting activities of phosphate-solubilizing Enterobacter asburiae as influenced by fungicides

Plant growth promoting activities of phosphate-solubilizing Enterobacter asburiae as influenced by fungicides

Biodegradation of Trifluralin in Harran Soil

Genes similar to naphthalene dioxygenase genes in trifluralin‐degrading bacteria

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