Biodegradation of polyhydroxyalkanoates (PHAs) in the South China Sea and identification of PHA-degrading bacteria

Volova, Tatiana G.; Boyandin, Anatoly N.; Vasil’ev, A. D.; Karpov, V. A.; Кожевников, И. В.; Prudnikova, Svetlana V.; Rudnev, V.P.; Xuân, Bùi Bá; Dũng, Vũ Việt; Гительзон, И. И.

doi:10.1134/s0026261711020184

Cited by 51 publications

(37 citation statements)

References 19 publications

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“…In contrast, Enterobacter cloacae sp. IBP-V001 isolated from the South China Sea was found to degrade PHA instead (Volova et al 2011). Another species, Enterobacter aerogenes 12Bi, was able to produce PHB from domestic wastewater (Ceyhan and Ozdemir 2011).…”

Section: Bacterial Identification and Diversity Analysismentioning

confidence: 99%

Bioprospecting of polyhydroxyalkanoates-producing bacteria from Indonesian marine environment

2019

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Abstract. Tan WA, Wijaya I, Purwadaria T. 2019. Bioprospecting of polyhydroxyalkanoates-producing bacteria from Indonesian marine environment. Biodiversitas 20: 1309-1315. Polyhydroxyalkanoates (PHA) are potential alternates to conventional synthetic plastics. PHA production in bacteria involves PHA synthase gene encoded by phaC. In this study, we isolated PHA-producing bacteria from the coastline and 1 mile from the coastline of three beaches in Indonesia. Further phaC detection and characterization of PHA production were conducted. The isolates were subjected to phylogenetic analysis based on 16S rDNA. Red Nile staining on minimal agar revealed that twenty-three isolates showed orange fluorescent, which indicated that they accumulated PHA in their cells. PCR detection showed the presence of PHA synthase class I-encoding gene phaC in twelve isolates. One representative amplicon was sequenced to verify its identity, in which it shared 86% similarity with the PHA synthase class I-encoding gene from an uncultured bacterium. Interestingly, the production of PHA in isolate ST.PA.75, which was closely related to Vibrio sp., was 2.1-fold higher than that in the Ralstonia eutropha JMP134 control. Three isolates showed similarity with bacterial genera and/or species for which PHA producing phenotypes had never been described before TP.SWC.20, which was closely related to Microbacterium arborescens, as well as TP.SWC.33 and TP.SWC.85, which were similar to Psychrobacter spp. Phylogenetic analysis showed that the PHA producing isolates were clustered into three phyla: γ-Proteobacteria, Actinomycetes, and Bacilli. A majority of the isolates (75%) were related to γ-Proteobacteria. In this study, we uncovered diverse novel promising strains for use in the production of PHA as a more environmentally-friendly alternative to hydrocarbon-based plastics.

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Section: Bacterial Identification and Diversity Analysismentioning

confidence: 99%

Bioprospecting of polyhydroxyalkanoates-producing bacteria from Indonesian marine environment

2019

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“…Only a limited number of studies focus on the degradability of biodegradable polymers in water sources and show disputable results as different methods were used for following the degradability . In many studies, samples were placed in a perforated basket in seawater (SW), and weight change of the leftover material is noted making no demarcation between the weight loss due to biodegradation or simply due to the disintegration of the samples to microplastics getting lost in the sea as secondary microplastics . The studies under controlled conditions using standard characterization techniques are required to understand the degradation behavior and to provide a comparative basis.…”

Section: Introductionmentioning

confidence: 99%

Fate of So‐Called Biodegradable Polymers in Seawater and Freshwater

et al. 2017

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The stability of polymers with C—C and stable C—heteroatom backbones against chemicals, hydrolysis, temperature, light, and microbes has challenged society with the problem of accumulation of plastic waste and its management worldwide. Given careless disposal of plastic waste, large amounts of plastic litter accumulate in the environment and disintegrate into microplastics. One of the questions frequently raised in the recent times is if so‐called biodegradable polymers can substitute conventional polymers for several applications and help to tackle this challenge. The answer is not so simple as biodegradability is a certified property occurring only under certain environmental conditions and therefore requires systematic study. As a first step, this study focusses on comparative degradation studies of six polymers (five taken from the so‐called biodegradable polyesters, including poly(lactic‐ co ‐glycolic acid) (PLGA), polycaprolactone (PCL), polylactic acid (PLA), poly(3‐hydroxybutyrate) (PHB), Ecoflex, and one well‐known non‐degradable polymer poly(ethylene terephthalate) (PET) in artificial seawater and freshwater under controlled conditions for 1 year. Only amorphous PLGA shows 100% degradation as determined by weight loss, change in molar mass with time, NMR, electron microscopy, and high‐performance liquid chromatography. This is a step forward in understanding the degradability of polyesters required for the design of environmentally friendly novel polymers for future use.

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“…Degradation is assessed by measuring the decay of relevant physical properties such as: changes in molecular weight mass and molecular mass distribution, tensile properties, mass loss, morphological changes, etc. (Rutkowska et al, 1998, 2002a,b; Imam et al, 1999; Tsuji and Suzuyoshi, 2002a,b, 2003; O’Brine and Thompson, 2010; Guzman et al, 2011; Sekiguchi et al, 2011; Volova et al, 2011). Rutkowska et al (1998, 2002a,b) tested some biopolymers in the waters of Gdynia Harbor (Baltic Sea).…”

Section: Introductionmentioning

confidence: 99%

Laboratory Test Methods to Determine the Degradation of Plastics in Marine Environmental Conditions

Tosin¹,

Weber²,

Siotto³

et al. 2012

Front. Microbio.

156

104

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In this technology report, three test methods were developed to characterize the degradation of plastic in marine environment. The aim was to outline a test methodology to measure the physical and biological degradation in different habitats where plastic waste can deposit when littered in the sea. Previously, research has focused mainly on the conditions encountered by plastic items when floating in the sea water (pelagic domain). However, this is just one of the possible habitats that plastic waste can be exposed to. Waves and tides tend to wash up plastic waste on the shoreline, which is also a relevant habitat to be studied. Therefore, the degradation of plastic items buried under sand kept wet with sea water has been followed by verifying the disintegration (visual disappearing) as a simulation of the tidal zone. Most biodegradable plastics have higher densities than water and also as a consequence of fouling, they tend to sink and lay on the sea floor. Therefore, the fate of plastic items lying on the sediment has been followed by monitoring the oxygen consumption (biodegradation). Also the effect of a prolonged exposure to the sea water, to simulate the pelagic domain, has been tested by measuring the decay of mechanical properties. The test material (Mater-Bi) was shown to degrade (total disintegration achieved in less than 9 months) when buried in wet sand (simulation test of the tidal zone), to lose mechanical properties but still maintain integrity (tensile strength at break = −66% in 2 years) when exposed to sea water in an aquarium (simulation of pelagic domain), and substantially biodegrade (69% in 236 days; biodegradation relative to paper: 88%) when located at the sediment/sea water interface (simulation of benthic domain). This study is not conclusive as the methodological approach must be completed by also determining degradation occurring in the supralittoral zone, on the deep sea floor, and in the anoxic sediment.

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Biodegradation of polyhydroxyalkanoates (PHAs) in the South China Sea and identification of PHA-degrading bacteria

Cited by 51 publications

References 19 publications

Bioprospecting of polyhydroxyalkanoates-producing bacteria from Indonesian marine environment

Bioprospecting of polyhydroxyalkanoates-producing bacteria from Indonesian marine environment

Fate of So‐Called Biodegradable Polymers in Seawater and Freshwater

Laboratory Test Methods to Determine the Degradation of Plastics in Marine Environmental Conditions

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