Biodegradation of polyhydroxyalkanoates by soil microbial communities of different structures and detection of PHA degrading microorganisms

Boyandin, Anatoly N.; Prudnikova, Svetlana V.; Филипенко, М. Л.; Khrapov, E. A.; Vasil’ev, A. D.; Volova, Tatiana G.

doi:10.1134/s0003683812010024

Cited by 72 publications

(39 citation statements)

References 26 publications

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“…Such PHA production rate appears to be the highest as recorded for all isolated bacteria so far. This result is similar with early reporting of PHA production using different species of Bacillus and its possible applications [28][29][30]. In addition to that, 70.5 % PHA production was observed [10] using Bacillus sp.…”

Section: Extraction and Quantitative Analysis Of Phassupporting

confidence: 94%

Production of Polyhydroxyalkanoates (PHAs) by Bacillus Strain Isolated from Waste Water and Its Biochemical Characterization

Mohapatra

Mohanta

Sarkar

et al. 2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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Municipal sewage triggers a stress prone environment to accumulate polyhydroxyalkanoates (PHAs) in the cytosol of bacteria. In view of that, different Bacillus species were isolated from municipal sewage and screened for evaluating their efficacy of PHA production. Growth parameters such as temperature, pH, glucose concentration and carbon nitrogen combinations were optimized with respect to higher biomass production as it is analogous to PHA accumulation. Under optimized conditions, the Bacillus species produced 3.09 g/L of PHAs which was estimated as a higher yield in comparison to other similar strains. Fourier transform infrared spectroscopic analysis of the extracted polyhydroxybutyrate confirmed the distinct peak corresponding to C=O group, whereas proton nuclear magnetic resonance ( 1 H NMR) and differential scanning colorimetric analysis exhibited detailed insight of its chemical structure and properties by reflecting monomeric unit. The high yielding bacterial isolate was identified by 16S rDNA sequencing and the sequence was confirmed as Bacillus subtilis with an accession no. KP172548 after submission to NCBI data base. The potential bacterium may be further exploited for cost effective and mass scale production of biopolymer.

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Section: Extraction and Quantitative Analysis Of Phassupporting

confidence: 94%

Production of Polyhydroxyalkanoates (PHAs) by Bacillus Strain Isolated from Waste Water and Its Biochemical Characterization

Mohapatra

Mohanta

Sarkar

et al. 2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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“…Биодеградация П3ГБ была оценена во многих экосистемах, таких как почва (Doi, 1992;Mergaert, 1993), осадок сточных вод (Mergaert, 1994), морская вода (Mukai, 1994;Mergaert, 1995) (Прудникова, Волова, 2012;Boyandin et al, 2012а;2012b;2013). Работа выполнена при поддержке гранта Российского научного фонда № 14-26-00039.…”

Section: Introductionunclassified

Microbial Degradation of Poly-3-Hydroxybutyrate in Samples of Agrogenically Changed Soils

Vinogradova¹,

Prudnikova²,

Zobova³

et al. 2015

J. Sib. Fed. Univ., Biol.

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“…An integrated approach to the investigation of PHA degradation by soil microorganisms under natural conditions was employed in publications by Boyandin et al (2012Boyandin et al ( , 2013. The authors studied this process in different climates and soils, taking into account the diversity of soil microbial communities, shapes of polymer samples and methods of their preparation, and the chemical composition of the PHAs tested.…”

Section: An Integrated Approach To the Investigation Of Pha Degradatimentioning

confidence: 99%

Biodegradation of Polyhydroxyalkanoates in Natural Soils

Boyandin¹,

Volova²,

Бояндин³

et al. 2015

J. Sib. Fed. Univ., Biol.

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Biodegradation of polyhydroxyalkanoates by soil microbial communities of different structures and detection of PHA degrading microorganisms

Cited by 72 publications

References 26 publications

Production of Polyhydroxyalkanoates (PHAs) by Bacillus Strain Isolated from Waste Water and Its Biochemical Characterization

Production of Polyhydroxyalkanoates (PHAs) by Bacillus Strain Isolated from Waste Water and Its Biochemical Characterization

Microbial Degradation of Poly-3-Hydroxybutyrate in Samples of Agrogenically Changed Soils

Biodegradation of Polyhydroxyalkanoates in Natural Soils

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