Biodegradation of polycyclic aromatic hydrocarbons by Sphingomonas sp. enhanced by water-extractable organic matter from manure compost

Kobayashi, Takayuki; Murai, Yasushi; Takagi, Kenji; Iimura, Yasufumi

doi:10.1016/j.scitotenv.2009.06.041

Cited by 53 publications

(30 citation statements)

References 28 publications

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“…Malic acid, oxalic acid, and citric acid enhanced the degradation of PHE through increasing the solubility of PHE, consistent with the results of Kobayashi et al [38] and Chen et al [39]. These authors reported that the degradation of PAHs was improved through some organic molecules, reflecting the increasing solubility of PAHs.…”

Section: Resultssupporting

confidence: 88%

Biodegradation of Mixed PAHs by PAH-Degrading Endophytic Bacteria

Zhu

Waigi

et al. 2016

IJERPH

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Endophytic bacteria can promote plant growth, induce plant defence mechanisms, and increase plant resistance to organic contaminants. The aims of the present study were to isolate highly PAH-degrading endophytic bacteria from plants growing at PAH-contaminated sites and to evaluate the capabilities of these bacteria to degrade polycyclic aromatic hydrocarbons (PAHs) in vitro, which will be beneficial for re-colonizing target plants and reducing plant PAH residues through the inoculation of plants with endophytic bacteria. Two endophytic bacterial strains P1 (Stenotrophomonas sp.) and P3 (Pseudomonas sp.), which degraded more than 90% of phenanthrene (PHE) within 7 days, were isolated from Conyza canadensis and Trifolium pretense L., respectively. Both strains could use naphthalene (NAP), PHE, fluorene (FLR), pyrene (PYR), and benzo(a)pyrene (B(a)P) as the sole sources of carbon and energy. Moreover, these bacteria reduced the contamination of mixed PAHs at high levels after inoculation for 7 days; strain P1 degraded 98.0% NAP, 83.1% FLR, 87.8% PHE, 14.4% PYR, and 1.6% B(a)P, and strain P3 degraded 95.3% NAP, 87.9% FLR, 90.4% PHE, 6.9% PYR, and negligible B(a)P. Notably, the biodegradation of PAHs could be promoted through additional carbon and nitrogen nutrients; therein, beef extract was suggested as the optimal co-substrate for the degradation of PAHs by these two strains (99.1% PHE was degraded within 7 days). Compared with strain P1, strain P3 has more potential for the use in the removal of PAHs from plant tissues. These results provide a novel perspective in the reduction of plant PAH residues in PAH-contaminated sites through inoculating plants with highly PAH-degrading endophytic bacteria.

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Section: Resultssupporting

confidence: 88%

Biodegradation of Mixed PAHs by PAH-Degrading Endophytic Bacteria

Zhu

Waigi

et al. 2016

IJERPH

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“…Many studies have proved that the sequestration of pollutants increases significantly with time in the aging processes [7,8]. Thus, sorption of organic pollutant from soil aggregates, which influences the bioavailability of the contaminant, is a critical factor for microbial bioremediation of the soil contaminated by PAHs [9].…”

Section: Introductionmentioning

confidence: 99%

Insight into the Modulation of Dissolved Organic Matter on Microbial Remediation of PAH-Contaminated Soils

et al. 2015

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Microorganisms play a key role in degradation of polycyclic aromatic hydrocarbons (PAHs) in environments. Dissolved organic matter (DOM) can enhance microbial degradation of PAHs in soils. However, it is not clear how will the soil microbial community respond to addition of DOM during bioremediation of PAH-contaminated soils. In this study, DOMs derived from various agricultural wastes were applied to remediate the aging PAH-contaminated soils in a 90-day microcosm experiment. Results showed that the addition of DOMs offered a more efficient and persistent elimination of soil PAHs compared to the control which had no DOM addition. PAH removal effects were different among treatments with various DOMs; the addition of DOMs with high proportion of hydrophobic fraction could remove PAHs more efficiently from the soil. Low-molecular-weight (LMW) PAHs were more easily eliminated than that with high-molecularweight (HMW). Addition of DOMs significantly increased abundance of 16S ribosomal RNA (rRNA), pdo1, nah, and C12O genes and obviously changed community compositions of nah and C12O genes in different ways in the PAHcontaminated soil. Phylogenetic analyses of clone libraries exhibited that all of nah sequences and most of C12O sequences were affiliated into Gammaproteobacteria and Betaproteobacteria. These results suggested that external stimuli produced by DOMs could enhance the microbial degradation of PAHs in soils through not only solubilizing PAHs but also altering abundance and composition of indigenous microbial degraders. Our results reinforce the understanding of role of DOMs in mediating degradation of PAHs by microorganims in soils.

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“…The same it is possible to say for the strains belonging to the genus Sphingomonas (Shi et al 2001;Kobayashi et al 2009). Recently, also an Arthrobacter oxydans isolate displayed satisfactory PAH biotransformation and sorption properties (Thion et al 2012).…”

Section: Resultsmentioning

confidence: 92%

Diversity and PAH growth abilities of bacterial strains isolated from a contaminated soil in Slovakia

et al. 2013

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Different abandoned industrial areas contaminated by polycyclic aromatic hydrocarbons (PAHs) are present in Slovakia. These environmental burdens are very dangerous to the health of human and environment. The bioremediation, based on the use of hydrocarbons degrading microorganisms, is a promising strategy to sanitize these polluted sites. The aim of this investigation was to assess the bacterial diversity of a PAHs-contaminated soil and to select the potential hydrocarbonoclastic bacteria which can be used for different bioremediation approaches. The bacterial strains were isolated on minimal medium agar supplemented with a mixture of PAHs. Seventy-three isolated strains were grouped by ribosomal interspacer analysis in 15 different clusters and representatives of each cluster were identified by 16S rRNA sequencing. The PAHs degradation abilities of all bacterial isolates were estimated by the 2,6-dichlorophenol indophenol assay and by their growth on minimal broth amended with a mixture of PAHs. Different kinds of strains, members of the genus Pseudomonas, Enterobacter, Bacillus, Arthrobacter, Acinetobacter and Sphingomonas, were isolated from the contaminated soil. Four isolates (Pseudomonas putida, Arthrobacter oxydans, Sphingomonas sp. and S. paucimobilis) showed promising PAHs-degrading abilities and therefore their possible employing in bioremediation strategies.

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Biodegradation of polycyclic aromatic hydrocarbons by Sphingomonas sp. enhanced by water-extractable organic matter from manure compost

Cited by 53 publications

References 28 publications

Biodegradation of Mixed PAHs by PAH-Degrading Endophytic Bacteria

Biodegradation of Mixed PAHs by PAH-Degrading Endophytic Bacteria

Insight into the Modulation of Dissolved Organic Matter on Microbial Remediation of PAH-Contaminated Soils

Diversity and PAH growth abilities of bacterial strains isolated from a contaminated soil in Slovakia

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