Biodegradation of phenol via meta cleavage pathway triggers de novo TAG biosynthesis pathway in oleaginous yeast

Patel, Alok; Sartaj, Km; Arora, Neha; Pruthi, Vikas; Pruthi, Parul A.

doi:10.1016/j.jhazmat.2017.07.013

Cited by 64 publications

(19 citation statements)

References 51 publications

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“…Cassia fistula L. fruit pulp 53.18 [42] Hemp seed aqueous extract 55.56 [44] Phenol 1 g/L + Glucose (7%) 64.92 [120] Hydrophobic waste (clarified butter sediment waste medium 70.74 [7] Trichosporon fermentans CICC 1368 pre-treated waste sweet potato vines under simultaneous saccharification and fermentation (SSF) [121] Rhodosporidium toruloides Brewers' spent grain [45] Lipomyces starkeyi Xylose and glucose [122] Rhodotorula glutinis Monosodium glutamate with glucose [123] Cryptococcus…”

Section: Oleaginous Bacteriamentioning

confidence: 99%

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

et al. 2020

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Microorganisms are known to be natural oil producers in their cellular compartments. Microorganisms that accumulate more than 20% w/w of lipids on a cell dry weight basis are considered as oleaginous microorganisms. These are capable of synthesizing vast majority of fatty acids from short hydrocarbonated chain (C6) to long hydrocarbonated chain (C36), which may be saturated (SFA), monounsaturated (MUFA), or polyunsaturated fatty acids (PUFA), depending on the presence and number of double bonds in hydrocarbonated chains. Depending on the fatty acid profile, the oils obtained from oleaginous microorganisms are utilized as feedstock for either biodiesel production or as nutraceuticals. Mainly microalgae, bacteria, and yeasts are involved in the production of biodiesel, whereas thraustochytrids, fungi, and some of the microalgae are well known to be producers of very long-chain PUFA (omega-3 fatty acids). In this review article, the type of oleaginous microorganisms and their expertise in the field of biodiesel or omega-3 fatty acids, advances in metabolic engineering tools for enhanced lipid accumulation, upstream and downstream processing of lipids, including purification of biodiesel and concentration of omega-3 fatty acids are reviewed.

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Section: Oleaginous Bacteriamentioning

confidence: 99%

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

et al. 2020

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“…Undoubtedly, the rapid development of industrialization is always associated with an increase in the complexity and waste toxicities, which are causing a cost, which is paid in terms of environmental pollution (Ali et al, 2018(Ali et al, , 2020a. It is approximated that 10,000 different dyes, with an estimated annual production of 280,000 tons, are commercially available worldwide; and azo dyes represent over 60% of the total dyes (Patel et al, 2017;Pattanaik et al, 2020). It is estimated that 20-50% of these dyes remain unfixed during the dyeing processes and ultimately end up in the dye effluents (Giovanella et al, 2020), leading to severe pollution of water supplies in the vicinity of dyeing industries (Neetha et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Performance of a Newly Isolated Salt-Tolerant Yeast Strain Sterigmatomyces halophilus SSA-1575 for Azo Dye Decolorization and Detoxification

et al. 2020

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The effective degradation of hazardous contaminants remains an intractable challenge in wastewater processing, especially for the high concentration of salty azo dye wastewater. However, some unique yeast symbionts identified from the termite gut system present an impressive function to deconstruct some aromatic compounds, which imply that they may be valued to work on the dye degradation for various textile effluents. In this investigation, a newly isolated and unique yeast strain, Sterigmatomyces halophilus SSA-1575, was identified from the gut system of a wood-feeding termite (WFT), Reticulitermes chinensis. Under the optimized ambient conditions, the yeast strain SSA-1575 showed a complete decolorization efficiency on Reactive Black 5 (RB5) within 24 h, where this azo dye solution had a concentration of a 50 mg/L RB5. NADH-dichlorophenol indophenol (NADH-DCIP) reductase and lignin peroxidase (LiP) were determined as the key reductase and oxidase of S. halophilus SSA-1575. Enhanced decolorization was recorded when the medium was supplemented with carbon and energy sources, including glucose, ammonium sulfate, and yeast extract. To understand a possible degradation pathway well, UV-Vis spectroscopy, FTIR and Mass Spectrometry analyses were employed to analyze the possible decolorization pathway by SSA-1575. Determination of relatively high NADH-DCIP reductase suggested that the asymmetric cleavage of RB5 azo bond was mainly catalyzed by NADH-DCIP reductase, and finally resulting in the formation of colorless aromatic amines devoid of any chromophores. The ecotoxicology assessment of RB5 after a decolorization processing by SSA-1575, was finally conducted to evaluate the safety of its metabolic intermediates from RB5. The results of Microtox assay indicate a capability of S. halophilus SSA-1575, in the detoxification of the toxic RB5 pollutant. This study revealed the effectiveness of halotolerant yeasts in the eco-friendly remediation of hazardous pollutants and dye wastewater processing for the textile industry.

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“…The acquisition and retention of characteristics of adaptability to various stresses by microbes in extreme environments are essentially found in the adaptive evolution process of the anti-stress function at the gene level, which means that it can be transmitted to descendants through asexual reproduction to maintain the reproduction of its population[ 30 ]. Interestingly, Patel A et al found that a phenol degrading yeast, Rhodosporidium kratochvilovae HIMPA1, could transform phenol into lipid granules, which could help to enhance its resistance to cold and salt when stored in the body [ 31 ]. Thus, it is not difficult to guess that for a phenol degradation strain in the natural environment, phenol may not only serve as an energy source but also play an important role in other aspects of the survival of the strain.…”

Section: Discussionmentioning

confidence: 99%

Degradation of phenol via ortho-pathway by Kocuria sp. strain TIBETAN4 isolated from the soils around Qinghai Lake in China

Ali

Liu

et al. 2018

PLoS ONE

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Based on the feature of high-altitude permafrost topography and the diverse microbial ecological communities of the Qinghai-Tibetan Plateau, soil samples from thirteen different collection points around Qinghai lake were collected to screen for extremophilic strains with the ability to degrade phenol, and one bacterial strain recorded as TIBETAN4 that showed effective biodegradation of phenol was isolated and identified. TIBETAN4 was closely related to Kocuria based on its observed morphological, molecular and biochemical characteristics. TIBETAN4 grew well in the LB medium at pH 7–9 and 0–4% NaCl showing alkalophilicity and halophilism. The isolate could also tolerate up to 12.5 mM phenol and could degrade 5 mM phenol within 3 days. It maintained a high phenol degradation rate at pH 7–9 and 0–3% NaCl in MSM with 5 mM phenol added as the sole carbon source. Moreover, TIBETAN4 could maintain efficient phenol degradation activity in MSM supplemented with both phenol and glucose and complex water environments, including co-culture Penicillium strains or selection of non-sterilized natural lake water as a culture. It was found that TIBETAN4 showed enzymatic activity of phenol hydroxylase and catechol 1,2-dioxygenase after induction by phenol and the corresponding genes of the two enzymes were detected in the genome of the isolate, while catechol 2,3-dioxygenase or its gene was not, which means there could be a degradation pathway of phenol through the ortho-pathway. The Q-PCR results showed that the transcripts of both the phenol hydroxylase gene and catechol 1,2-dioxygenase gene were up-regulated under the stimulation of phenol, demonstrating again that the strain degraded phenol via ortho-degradation pathway.

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Biodegradation of phenol via meta cleavage pathway triggers de novo TAG biosynthesis pathway in oleaginous yeast

Cited by 64 publications

References 51 publications

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

Performance of a Newly Isolated Salt-Tolerant Yeast Strain Sterigmatomyces halophilus SSA-1575 for Azo Dye Decolorization and Detoxification

Degradation of phenol via ortho-pathway by Kocuria sp. strain TIBETAN4 isolated from the soils around Qinghai Lake in China

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