Biodegradation of phenol at high initial concentrations in two-phase partitioning batch and fed-batch bioreactors

Collins, Lisa D.; Daugulis, Andrew J.

doi:10.1002/(sici)1097-0290(19970705)55:1<155::aid-bit16>3.0.co;2-l

Cited by 103 publications

(21 citation statements)

References 21 publications

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“…These pollutants are usually treated in activated sludge processes because many aerobic bacteria and fungi are able to use phenol as a source of carbon and energy (Rebhun and Galil 1988;Watanabe et al, 1996). Biodegradation of phenol, therefore, has long been the subject of numerous investigations (Ruiz-Rrdaz et al, 2001;Chang et al, 1998;Fava et al, 1995;Abd-El-Haleem et al, 2003;Dean-Ross, 1989;Solomon et al, 1994;Ahmed et al, 1995;Alleman et al, 1995;Collins and daugulis, 1997;Fulthorpe and Allen, 1995;Lin et al, 1990;Morris and Lester, 1994;Ryu et al, 2000;Wang et al, 1996). A typical pathway for metabolizing an aromatic compound like phenol is to dihydroxylate the benzene ring to form a catechol derivative and then to open the ring through ortho or meta oxidation.…”

supporting

confidence: 82%

Detection of meta - and ortho -cleavage dioxygenases in bacterial phenol-degraders

Zaki

2006

Journal of Applied Sciences and Environmental Management

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ABSTRACT:In the last five years, in our lab, several bacterial genera capable of degrading phenol as sole carbon source were isolated from different Egyptian ecosystems. Phenol mineralization using these isolates was ranged from 55% to 0.4%. In the present work, randomly chosen representative strains; W-17, DF4 (Acinetobacter), Sea-8 (Stenotrophomonas), W-6 (Klebsiella), S-5 (Bacillus) and W-15 (Ralstonia) were analyzed for the type of ring-cleavage (ortho or meta) dioxygenase physiologically induced during growth in the presence of phenol as a sole carbon and energy source. The specific activities of the phenol-degrading enzymes phenol hydroxylase, catechol-1,2-dioxygenase and catechol-2,3-dioxygenase were investigated. In addition, whole-cell dioxygenases activities of the examined isolates were determined. Out of the results, only the Acinetobacter strains W-17 and DF-4 showed activity with the enzymes phenol hydroxylase and catechol-1,2-dioxygenase, which responsible for phenol degradation through ortho-cleavage pathway. In contrast, isolates S-5, Sea-8, W-6, W-15 and Pla-1 showed activity with the enzyme catalyzing the second step in the phenol degradation meta-cleavage pathway, catechol-2,3-dioxygenase. On the basis of our previous and present analysis, the investigated isolates are considered to have a good potential for application in remediation of phenol contaminated environments and industrial wastewater. @JASEM Phenol and phenolic compounds are of widespread use in many industries such as polymeric resin production and oil refining. As a result, these compounds are commonly encountered in industrial effluents and surface water. These pollutants are usually treated in activated sludge processes because many aerobic bacteria and fungi are able to use phenol as a source of carbon and energy (Rebhun and Galil 1988;Watanabe et al., 1996). Biodegradation of phenol, therefore, has long been the subject of numerous investigations (Ruiz-Rrdaz et al

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supporting

confidence: 82%

Detection of meta - and ortho -cleavage dioxygenases in bacterial phenol-degraders

Zaki

2006

Journal of Applied Sciences and Environmental Management

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“…Thus, it is observed that as the initial phenol concentration increased the duration of the lag phase increased; and thereby, prolonging the biodegradation time as a result of decrease in the rate of degradation. This observation is supported by the earlier works of Andrews (1968), Hill and Robinson (1975), Collins and Daugulis (1997), and Oboirien et al (2005). According to Prpich and Daugulis (2005), the rate of substrate consumption was suggested to be the most important measure of microbe performance.…”

Section: Resultsmentioning

confidence: 99%

“…It is an organic, aromatic compound that occurs naturally in the environment (Prpich and Daugulis, 2005), but is more commonly produced artificially from industrial activities such as petroleum processing, plastic manufacturing, resin production, pesticide production, steel manufacturing and the production of paints and varnish (Mahadevaswamy et al, 1997;Bandyopadhyay et al, 1998). This aromatic compound is water-soluble and highly mobile (Collins and Daugulis, 1997) and as such waste waters generated from these industrial activities contain high concentrations of phenolic compounds (Chang et al, 1998) which eventually may reach down to streams, rivers, lakes, and soil, which represent a serious ecological problem due to their widespread use and occurrence throughout the environment (Fava et al, 1995). Phenol is a listed priority pollutant by the U.S. Environmental Protection Agency (EPA, 1979) and is considered to be a toxic compound by the Agency for Toxic substances and Disease Registry (ATSDR, 2003).…”

Section: Introductionmentioning

confidence: 99%

Kinetics of batch microbial degradation of phenols by indigenous Pseudomonas fluorescence

Agarry

Solomon

2008

Int. J. Environ. Sci. Technol.

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ABSTRACT:The potential of various organisms to metabolize organic compounds has been observed to be a potentially effective means in disposing of hazardous and toxic wastes. Phenols and their compounds have long been recognized as one of the most recalcitrant and persistent organic chemicals in the environment. The bioremediation potential of an indigenous Pseudomonas fluorescence was studied in batch culture using synthetic phenol in water in the concentration range of (100 -500) mg/L as a model limiting substrate. The effect of initial phenol concentration on the degradation process was investigated. Phenol was completely degraded at different cultivation times for the different initial phenol concentrations. Increasing the initial phenol concentration from 100 mg/L to 500 mg/L increased the lag phase from 0 to 66 h and correspondingly prolonged the degradation process from 84 h to 354 h. There was decrease in biodegradation rate as initial phenol concentration increased. Fitting data into Monod kinetic model showed the inhibition effect of phenol The kinetic parameters have been estimated up to initial phenol concentration of 500 mg/ L. The r smax decreased and K s increased with higher concentration of phenol. The r smax has been found to be a strong function of initial phenol concentration. The culture followed substrate inhibition kinetics and the specific phenol consumption rates were fitted to Haldane, Yano and Koga, Aiba et al., Teissier and Webb models. Between the five inhibition models, the Haldane model was found to give the best fit. Therefore, the biokinetic constants estimated using these models showed good potential of the Pseudomonas fluorescence and the possibility of using it in bioremediation of phenol waste effluents.

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“…Of the several methods available for treatment of phenol, biological treatment is especially attractive because it has the potential to almost completely degrade phenol while producing innocuous end products with minimum secondary waste generation. Biodegradation of phenol has been extensively investigated, and several studies have shown that phenol can be aerobically degraded by a wide variety of microorganisms, including pure bacterial cultures (Collins andDaugulis, 1997, andHoyle et al, 1995), mixed bacterial cultures (Pawlowsky andHowell, 1973, and, yeast (Neujahr andVarga, 1970, andYang andHumphrey, 1970), and filamentous fungi (Garcia Garcia et al, 1997, andHofrichter et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Substrate Inhibition Kinetics of Phenol Biodegradation

Goudar¹,

Ganji²,

Pujar³

et al. 2000

Water Environment Research

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Phenol biodegradation was studied in batch experiments using an acclimated inoculum and initial phenol concentrations ranging from 0.1 to 1.3 g/L. Phenol depletion and associated microbial growth were monitored over time to provide information that was used to estimate the kinetics of phenol biodegradation. Phenol inhibited biodegradation at high concentrations, and a generalized substrate inhibition model based on statistical thermodynamics was used to describe the dynamics of microbial growth in phenol. For experimental data obtained in this study, the generalized substrate inhibition model reduced to a form that is analogous to the Andrews equation, and the biokinetic parameters max, maximum specific growth; Ks', saturation constant; and Ki', inhibition constant were estimated as 0.251 h 2 1 , 0.011 g/L, and 0.348 g/L, respectively, using a nonlinear least squares technique. Given the wide variability in substrate inhibition models used to describe phenol biodegradation, an attempt was made to justify selection of a particular model based on theoretical considerations. Phenol biodegradation data from nine previously published studies were used in the generalized substrate inhibition model to determine the appropriate form of the substrate inhibition model. In all nine cases, the generalized substrate inhibition model reduced to a form analogous to the Andrews equation suggesting the suitability of the Andrews equation to describe phenol biodegradation data.

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Biodegradation of phenol at high initial concentrations in two-phase partitioning batch and fed-batch bioreactors

Cited by 103 publications

References 21 publications

Detection of <i>meta</i> - and <i>ortho</i> -cleavage dioxygenases in bacterial phenol-degraders

Detection of <i>meta</i> - and <i>ortho</i> -cleavage dioxygenases in bacterial phenol-degraders

Kinetics of batch microbial degradation of phenols by indigenous Pseudomonas fluorescence

Substrate Inhibition Kinetics of Phenol Biodegradation

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