Biodegradation of Petroleum Compound Using the Bacterium Bacillus subtilis

Darsa, K.V.; Thatheyus, A. Joseph; Ramya, D.

doi:10.17311/sciintl.2014.20.25

Cited by 41 publications

(36 citation statements)

References 8 publications

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“…This shows that the bacteria were more active at a neutral pH. This observation is in agreement with the literatures, that microorganisms achieve better efficiency at pH 6 -8, with the optimum obtainable at pH 7 (Agarry and Aremu, 2012;Darsa et al, 2014;). Figure 2 shows the percentage of naphthalene adsorbed and biodegraded at varying adsorbent doses set at 2, 3 and 5 g respectively with a fixed naphthalene concentration of 50 mg/L and a pH of 7.…”

Section: Resultssupporting

confidence: 92%

Biodegradation of Naphthalene using Pseudomonas putida and Bacillus subtilis Immobilized on Snail Shell

Aina¹,

Agbaji²,

Nwokem³

2018

Journal of Applied Sciences and Environmental Management

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This study investigated snail shell as a carrier to immobilize Pseudomonas putida and Bacillus subtilis isolated from refinery effluent for the degradation of naphthalene in synthetic wastewater at various process conditions such as initial naphthalene concentration, pH, adsorbent dosage and ambient temperature of 30 o C in batch mode. The results showed that the adsorption and the biodegradation capacity increased with increase in naphthalene concentration, where 73.11%, 74.46% and 65.20% of the optimum concentration (50 mg/L) were removed by immobilized Pseudomonas putida, Bacillus subtilis, and snail shell respectively after 72 hours incubation. The optimal degradation occurred at the adsorbent dosage of 2 g at pH 9 and pH 7 for the adsorption and biodegradation respectively. The results were well fitted to both Langmuir and Freundlich models. Therefore, snail shell can be employed as a low-cost adsorbent and solid support matrix for immobilizing microorganisms in remediating hydrocarbon contaminants.

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Section: Resultssupporting

confidence: 92%

Biodegradation of Naphthalene using Pseudomonas putida and Bacillus subtilis Immobilized on Snail Shell

Aina¹,

Agbaji²,

Nwokem³

2018

Journal of Applied Sciences and Environmental Management

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“…Bacillus species are more tolerant to high levels of oils due to their resistant endospores. They are known to possess a more competent and active oil degrading enzymes than other biodegraders (Darsa et al 2014). Degradation of oil by these microbial consortia shows that they have specialized cometabolic capacities.…”

Section: Resultsmentioning

confidence: 99%

Identification and characterisation of oil sludge degrading bacteria isolated from compost

Ubani

Atagana

Thantsha

et al. 2016

Archives of Environmental Protection

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Compounds present in oil sludge such as polycyclic aromatic hydrocarbons (PAHs) are known to be cytotoxic, mutagenic and potentially carcinogenic. Microorganisms including bacteria and fungi have been reported to degrade oil sludge components to innocuous compounds such as carbon dioxide, water and salts. In the present study, we isolated different bacteria with PAH-degrading capabilities from compost prepared from oil sludge and animal manures. These bacteria were isolated on a mineral base medium and mineral salt agar plates. A total of 31 morphologically distinct isolates were carefully selected from 5 different compost treatments for identifi cation using polymerase chain reaction (PCR) of the 16S rRNA gene with specifi c primers (universal forward 16S-P1 PCR and reverse 16S-P2 PCR). The amplicons were sequenced and sequences were compared with the known nucleotides from the GenBank. The phylogenetic analyses of the isolates showed that they belong to 3 different clades; Firmicutes, Proteobacteria and Actinobacteria. These bacteria identifi ed were closely related to the genera Bacillus, Arthrobacter, Staphylococcus, Brevibacterium, Variovorax, Paenibacillus, Ralstonia and Geobacillus. The results showed that Bacillus species were predominant in all composts. Based on the results of the degradation of the PAHs in the composts and results of previous studies on bacterial degradation of hydrocarbons in oil, the characteristics of these bacterial isolates suggests that they may be responsible for the breakdown of PAHs of different molecular weights in the composts. Thus, they may be potentially useful for bioremediation of oil sludge during compost bioremediation.

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“…Laboratory experiments have shown that biosurfactant production is not inhibited by the presence of crude oil (Queiroga et al, 2003). Several experiments of bioremediation of soils polluted by hydrocarbons were performed with B. subtilis (Moran et al, 2000;Christova et al, 2004;Das and Mukherjee 2007;Wang et al, 2011;Jalilzadeh Yengejeh et al, 2014;Darsa et al, 2014). The strain P. fluorescens was used in a number of hydrocarbon biodegradation experiments.…”

mentioning

confidence: 99%

Characterization and Evaluation of the Potential of a Diesel-Degrading Bacterial Consortium Isolated from Fresh Mangrove Sediment

Lang

Destain

Delvigne

et al. 2016

Water Air Soil Pollut

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Hydrocarbons are ubiquitous and persistent organic pollutants in the environment. In wetlands and marine environments, particularly in mangrove ecosystems, their increase and significant accumulation result from human activities such as oil and gas exploration and exploitation operations. Remediation of these ecosystems requires the development of adequate and effective strategies. Natural attenuation, biostimulation, and bioaugmentation are all biological soil treatment techniques that can be adapted to mangroves. Our experiments were performed on samples of fresh mangrove sediments from the Cameroon estuary and mainly from the Wouri River in Cameroon. This study aims to assess the degradation potential of a bacterial consortium isolated from mangrove sediment. The principle of our bioremediation experiments is based on a series of tests designed to evaluate the potential of an active indigenous microflora and three exogenous pure strains, to degrade diesel with/without adding nutrients. The experiments were conducted in laboratory flasks and a greenhouse in microcosms. In one case, as in the other, the endogenous microflora showed that it was able to degrade diesel. Under stress of the pollutant, the endogenous microflora fits well enough in the middle to enable metabolism of the pollutant. However, the Rhodococcus strain was more effective over time. The degradation rate was 77 and 90 % in the vials containing the sterile sediments and non-sterile sediments, respectively. The results are comparable with those obtained in the microcosms in a greenhouse where only the endogenous microflora were used. The results of this study show that mangrove sediment contains an active microflora that can metabolize diesel. Indigenous and active microflora show an interesting potential for diesel degradation.

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Biodegradation of Petroleum Compound Using the Bacterium Bacillus subtilis

Cited by 41 publications

References 8 publications

Biodegradation of Naphthalene using Pseudomonas putida and Bacillus subtilis Immobilized on Snail Shell

Biodegradation of Naphthalene using Pseudomonas putida and Bacillus subtilis Immobilized on Snail Shell

Identification and characterisation of oil sludge degrading bacteria isolated from compost

Characterization and Evaluation of the Potential of a Diesel-Degrading Bacterial Consortium Isolated from Fresh Mangrove Sediment

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