Biodegradation of PAHs byBurkholderiasp. VITRSB1 Isolated from Marine Sediments

Abstract

The polycyclic aromatic hydrocarbons (PAHs) pollution to the environment is a major threat to the living organisms, and hence the degradation of these PAHs is necessary. Studies on PAHs degrading bacteria have focussed on terrestrial microbes and the potential of marine derived microbes is undermined. Herein we report the isolation and characterization of PAHs degrading Burkholderia sp. from lagoon sediments collected at the Southern coast of India. The strain was Gram negative, rod-shaped, motile, and ∼2–5 μm in length. Based on the phylogenetic data the strain was identified as Burkholderia and designated as VITRSB1. Initial PAHs degradation ability of the strain was assessed using basal salt medium supplemented with diesel, kerosene, toluene, aniline, naphthalene, and phenol. The strain was found to be effectively degrading kerosene, diesel, toluene, and aniline even at higher concentration (1%). However, naphthalene and aniline were degraded only at lower concentration (0.1%) and phenol, camphor, and DAP inhibited the growth of the strain. Furthermore, the degraded end products of the PAHs were determined using FTIR. Notably, none of the end products were found to be toxic to the biosphere. Our results indicate that the isolated Burkholderia sp. could be a prospective candidate for the effective degradation of selective PAHs.

Bioinformatic Approaches Including Predictive Metagenomic Profiling Reveal Characteristics of Bacterial Response to Petroleum Hydrocarbon Contamination in Diverse Environments

“…Pathogenic microorganisms are known to be important degraders of petroleum hydrocarbons. Several strains of infectious microbes such as Burkholderia 32 , Stenotrophomonas 33 , 34 and Mycobacterium 35 have been shown to harbor impressive capabilities for degradation of a variety of petroleum hydrocarbons. In the present study, pathogens such as Mycobacterium and Burkholderia , were identified as genus level biomarkers for C samples and Tb samples respectively.…”

Analysis of viral and bacterial communities in groundwater associated with contaminated land

“…Bacteria, with (strain-level) relatives able of biodegradation, that could also be affected by viral generalists found here include, for example, Hydrocarboniphaga sp. (by BGW-34) (Burback and Perry, 1993;Chatterjee and Bourquin, 1987;Manickam et al, 2007;Mattes et al, 2010;Palleroni et al, 2004;Ping et al, 2017;Poi et al, 2018;Revathy et al, 2015;Simarro et al, 2013).…”

An Indigenous Biosurfactant Producing Burkholderia cepacia with High Emulsification Potential towards Crude Oil

“…cepacia can degrade a wide variety of compounds as carbon and energy sources [13], including a variety of aromatic compounds [14,15] pesticides and herbicides [16]. It has the ability to degrade benzo(a) pyrene and other fused ring compounds [17], and biodegradation of heavy crude oil [18].…”