Biodegradation of Natural Rubber and Natural Rubber Products by Streptomyces sp. Strain CFMR 7

Nanthini, Jayaram; Sudesh, Kumar

doi:10.1007/s10924-016-0840-1

Cited by 26 publications

(17 citation statements)

References 20 publications

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“…As can be seen in the rubber utilization studies, minimal differences were observed for tyre samples, similar case was observed after incubating tyre samples for up to 9 months 22 . Unlike other rubber products, tyres are made from vulcanized rubber and ~30% carbon black for reinforcement which makes them more resistant to degradation.…”

Section: Discussionsupporting

confidence: 79%

Rubber Degrading Strains of Microtetraspora and Dactylosporangium

Basik

Nanthini

Yeo

et al. 2021

Preprint

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Latex clearing protein (lcp) found in Actinobacterial strains is reportedly critical for the initial oxidative cleavage of poly(cis-1,4-isoprene), the major polymeric unit of rubber. In this study, we screened 940 Actinobacterial strains isolated from various locations in Sarawak on NR latex agar and identified 18 strains from 5 genera that produced clearing zones and contained (latex clearing protein) lcp genes. We report here the first lcp genes from Microtetraspora sp. AC03309 (lcp 1 and lcp2) and Dactylosporangium sp. AC04546 (lcp1, lcp2, lcp3), together with their operon structure. Complete 16S rDNA gene sequence revealed that Dactylosporangium sp. AC04546 is 99% identical to Dactylosporangium sucinum RY35-23 whereas Microtetraspora sp. AC03309 is 98% identical to Microtetraspora glauca IFO14761. Morphological images and the spectrophotometric detection of aldehyde and keto groups in rubber samples incubated with the strains confirm the strains’ ability to degrade rubber-based products.

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Section: Discussionsupporting

confidence: 79%

Rubber Degrading Strains of Microtetraspora and Dactylosporangium

Basik

Nanthini

Yeo

et al. 2021

Preprint

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“…TTGTGATGTCCGACACGGTGTGCTGTGA TTGTGATCAAGGACACGATGTGTTCTCA TTGTGAGCACCGACACAGTGTGCTGTTA GGTGTGTCCTGAGTCACTCGATGCTATCA TTGTGACCCGAGCCACAGTGTGGTTCGA CATGTGTCCTGGACCACCCGGTGGTAACA TTGTGAAGTCCCGGCCGCCGTGCTCTCA GGTGTGTCCTGGACCACCTGGTGGTAACA TGGTGCTCGCCCCCGGAGCGTGCTTCCC TTGTGGCGTGAATCATGCTCTCCTGGGG TGGTCTTTTCTTCCCGTCTGTGGTCTCC CGGGTGATCCAAGACACACCGAAATCCCC C C C C A TTGTGATGCCAATCACTGTATGCTACTC A multiple alignment was performed in which bases with a conservation of at least 70% are highlighted. Strains in boldface are known to utilize rubber as a carbon source (8,(55)(56)(57). Genome sequences of all strains are available in the prescribed order under the following RefSeq IDs (NCBI):…”

Section: Resultsmentioning

confidence: 99%

Global Regulator of Rubber Degradation in Gordonia polyisoprenivorans VH2: Identification and Involvement in the Regulation Network

Witt

Oetermann

Parise

et al. 2020

Appl Environ Microbiol

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A cAMP receptor protein (CRPVH2) was detected as a global regulator in Gordonia polyisoprenivorans VH2 and was proposed to participate in the network regulating poly(cis-1,4-isoprene) degradation as a novel key regulator. CRPVH2 shares a sequence identity of 79% with GlxR, a well-studied global regulator of Corynebacterium glutamicum. Furthermore, CRPVH2 and GlxR have a common oligomerization state and similar binding motifs, and thus most likely have similar functions as global regulators. Size exclusion chromatography of purified CRPVH2 confirmed the existence as a homodimer with a native molecular weight of 44.1 kDa in the presence of cAMP. CRPVH2 bound to the TGTGAN6TCACT motif within the 131-bp intergenic region of divergently oriented lcp1VH2 and lcpRVH2, encoding a latex clearing protein and its putative repressor, respectively. DNase I footprinting assays revealed the exact operator size of CRPVH2 in the intergenic region (25 bp), which partly overlapped with the proposed promoters of lcpRVH2 and lcp1VH2. Our findings indicate that CRPVH2 represses the expression of lcpRVH2 while simultaneously directly or indirectly activating the expression of lcp1VH2 by binding the competing promoter regions. Furthermore, binding of CRPVH2 to upstream regions of additional putative enzymes of poly(cis-1,4-isoprene) degradation was verified in vitro. In silico analyses predicted 206 CRPVH2 binding sites comprising 244 genes associated with several functional categories, including carbon and peptide metabolism, stress response, etc. The gene expression regulation of several subordinated regulators substantiated the function of CRPVH2 as a global regulator. Moreover, we anticipate that the novel lcpR regulation mechanism by CRPs is widespread in other rubber-degrading actinomycetes. IMPORTANCE In order to develop efficient microbial recycling strategies for rubber waste materials, it is required that we understand the degradation pathway of the polymer and how it is regulated. However, only little is known about the transcriptional regulation of the rubber degradation pathway, which seems to be upregulated in the presence of the polymer. We identified a novel key regulator of rubber degradation (CRPVH2) that regulates several parts of the pathway in the potent rubber-degrader G. polyisoprenivorans VH2. Furthermore, we provide evidence for a widespread involvement of CRP regulators in the degradation of rubber in various other rubber-degrading actinomycetes. Thus, these novel insights into the regulation of rubber degradation are essential for developing efficient microbial degradation strategies for rubber waste materials by this group of actinomycetes.

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“…For instance, the demand for personal protective equipment related to the circulation of the SARS-CoV-2 virus has increased since 2020. Given the limited space in landfills, the high cost of incineration, and the high potential risk to health and the environment, the studies on the biodegradability of NR become fundamental [26][27][28] .…”

Section: Biocompatibility and Biodegradabilitymentioning

confidence: 99%

Latex and natural rubber: recent advances for biomedical applications

et al. 2022

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Recently, latex (NRL) and natural rubber (NR) from Hevea brasiliensis have emerged as promising biomaterials from renewable sources for biomedical applications. Although some attempts at commercial applications have been made, there is a need to comprehensively document the state-of-the-art of these biopolymers for biomedical applications and regenerative medicine. Here we present the recent advances in the development of NRL and NR as biomedical materials with potential properties including biocompatibility and biodegradability. Due to the angiogenic properties of NRL and NR, well-defined functional materials can be used for drug delivery systems (oral/transdermal), scaffolds for skin and bone regeneration, and dressings for wound healing. The incorporation of drugs, nanoparticles, cells, and others into NRL and NR polymer chains offers a wide range of applications such as dressings with antimicrobial activity and sustained release systems. Concluding remarks on the growth of these biomaterials for biomedical applications and regenerative medicine were discussed.

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Biodegradation of Natural Rubber and Natural Rubber Products by Streptomyces sp. Strain CFMR 7

Cited by 26 publications

References 20 publications

Rubber Degrading Strains of Microtetraspora and Dactylosporangium

Rubber Degrading Strains of Microtetraspora and Dactylosporangium

Global Regulator of Rubber Degradation in Gordonia polyisoprenivorans VH2: Identification and Involvement in the Regulation Network

Latex and natural rubber: recent advances for biomedical applications

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