Biodegradation of Naphthalene and Anthracene by Aspergillus glaucus Strain Isolated from Antarctic Soil

Abstract

Biotechnologies based on microbial species capable of destroying harmful pollutants are a successful way to solve some of the most important problems associated with a clean environment. The subject of investigation is the Antarctic fungal strain Aspergillus glaucus AL1. The culturing of the examined strain was performed with 70 mg of wet mycelium being inoculated in a Czapek Dox liquid medium containing naphthalene, anthracene, or phenanthrene (0.3 g/L) as the sole carbon source. Progressively decreasing naphthalene and anthracene concentrations were monitored in the culture medium until the 15th day of the cultivation of A. glaucus AL1. The degradation was determined through gas chromatography–mass spectrometry. Both decreased by 66% and 44%, respectively, for this period. The GC-MS analyses were applied to identify salicylic acid, catechol, and ketoadipic acid as intermediates in the naphthalene degradation. The intermediates identified in anthracene catabolism are 2-hydroxy-1-naphthoic acid, o-phthalic acid, and protocatechuic acid. The enzyme activities for phenol 2-monooxygenase (1.14.13.7) and catechol 1,2-dioxygenase (1.13.11.1) were established. A gene encoding an enzyme with catechol 1,2-dioxygenase activity was identified and sequenced (GeneBank Ac. No KM360483). The recent study provides original data on the potential of an ascomycete’s fungal strain A. glaucus strain AL 1 to degrade naphthalene and anthracene.

Diversity in storage age enables discrepancy in quality attributes and metabolic profile of Citrus grandis “Tomentosa” in China

“…Retinol metabolism pathway involved the metabolism of retinol to retinyl ester and retinal, retinal to retinoate, and retinal was further involved in the phototransduction pathway, the above pathways should be related to the decrease of vitamin A and retinal. Pyrocatechol was undergone many degradation pathways, such as naphthalene and anthracene degradation, that have been indicated to be mediated by microorganisms (Stoyanova et al., 2022), which enhancing the possibility of microbial action in CGT aging. 2‐Aminophenol was also involved in naphthalene and anthracene degradation pathway, manifesting by its metabolism to 2‐aminomuconate semialdehyde, and further participating in the tryptophan metabolism pathway.…”

Molecular advances in mycoremediation of polycyclic aromatic hydrocarbons: Exploring fungal bacterial interactions

“…F022 Dioxygenantion of 1,4 position C-atom to give naphthoquinones [28] Pleurotus eryngii Mineralize Naphthalene by dioxygenating C1−C4 atom [29] Pleurotus ostreatus Αand β-naphthol were initial degradation products which further metabolized to salycilic and benzoic acid [30] Ganoderma sp. Lower pathway metabolites benzoic acid, catechol, pthalic acid, and protocatechuic acid were obtained [31] Aspergillus Glaucus AL1 Phenol-2-monoxygenase and catechol-1,2-dioxygenase are involved in degradation [32] Phenanthrene Pleurotus ostreatus P-450 was mainly responsible for the activity [8] Irpex lacteus - [33] Ceratobasidium stevensii Degraded 89.51% of phenanthrene, phenanthrene removal, and MnP activity was not corelated [34] Polyporus sp. S133 Phenanthrene-9,10-quinone, 2,2-Diphenic acid, Phthalic acid, and protocatechuic acid were obtained as intermediates.…”

“…Pycnoporus sanguineus H1 Anthracene degradation rate decreased after piperonyl butoxide, P450 inhibitor addition [50] Aspergillus glaucus AL1 Phenol-2-monoxygenase and catechol-1,2-dioxygenase are involved in degradation [32] Pyrene Fusarium solani and Hypocrea lixii Metal tolerant strain degraded 60% of pyrene after 2 weeks [51] Pseudotrametes gibbose Increased degradation pyrene in the presence of cosubstrates was observed [52] Pycnoporus sanguineus H1 Pyrene degradation rate decreased in the presence of piperonyl butoxide [50] Coriolopsis byrsina APC5 4,5-Dihydroxypyrene, phenanthrene, benzoic acid, benzoic acid-2-hydroxy pentyl ester, pyruvic acid, phthalic acid Di isopropyl ester were detected as metabolites [53] Trichoderma sp. F03 Fungi degraded 78% pyrene in the presence of surfactant and glucose [54] Ceriporia lacerate RF-7 Degradation was increased in the presence of glucose as cosubstrate [55] Candida tropicalis Two metabolic pathways catalyzed by P450 and 1,2dioxygenase was seen [11] Benzo(a)pyrene Anthacophyllum discolor Degraded 75% of benzo(a)pyrene from medium [56] Armillaria F022 Glucose addition increased degradation 2.5-fold.…”

An Investigation into the Potential of a Penicillium Commune Strain to Eliminate Aromatic Compounds

“…The majority of studies on the removal of mono-and poly-aromatic compounds by fungi concern one of the mechanisms for their removal, which is connected to the presence of a lignolytic enzyme system or extracellular peroxidases (lignin peroxidase, manganese peroxidase, and laccase) [22,23]. Although not many, there are articles that report on the uptake of aromatic compounds used as the only carbon source in fungi cultivation [24][25][26][27][28].…”