Biodegradation of micropollutant naproxen with a selected fungal strain and identification of metabolites

Aracagök, Y. Doruk; Göker, Hakan; Cihangir, Nilüfer

doi:10.1515/znc-2016-0162

Cited by 27 publications

(14 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result indicated that metabolite formed by loss of methyl group (O-desmethylnaproxen, C 13 H 12 O 3 ). Besides LC/MS analysis we used 1 HNMR, and 13 CNMR analysis in our former study to identify this metabolite [14].…”

Section: Resultsmentioning

confidence: 99%

“…As described before, in our group's previous study O-desmethylnaproxen was obtained by Aspergillus niger spore suspension and identified by proton nuclear magnetic resonance ( 1 H NMR) and carbon-13 nuclear magnetic resonance ( 13 C NMR) [14].…”

Section: O-desmethylnaproxen Production and Isolationmentioning

confidence: 93%

“…As in our group's previous study, proteomic analysis of O-desmethylnaproxen was done with LC/MS method [14].…”

Section: Proteomic Analysismentioning

confidence: 99%

See 2 more Smart Citations

In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A Mouse Connective Tissue Fibroblast Cells

Akbay

Aracagök

Göker

et al. 2021

Hacettepe Journal of Biology and Chemistry

Self Cite

View full text Add to dashboard Cite

B u in vitro çalışmanın amacı, O-desmetilnaproksen'nin sitotoksik seviyesinin belirlenmesidir. Bu amaçla, L929 hücre hattı, fare bağ doku fibroblast hücreleri, 24-ve 48-saat boyunca farklı konsantrasyonlarda naproksen ve O-desmetilnaproksene maruz bırakılmıştır. Hücre canlılığı MTT ile test edilirken, apoptoz AO/PI çift boyama yöntemi ile belirlenmiştir. Kontrol grubu herhangi bir ajan içermeyen (sadece DMEM) ve % 100 hücre canlılığı olan grup olarak kabul edilmiştir. IC50 sonuçları, % 50 hücre ölümüne neden olan grubun en yüksek konsantrasyona sahip 0-desmetilnaproksen'de (0.7 ug / mL) ortaya çıktığı ancak naproksen konsantrasyonlarının, % 50 hücre ölümüne neden olmadığı tespit edilmiştir. Sonuç olarak, idrarla vücuttan uzaklaştırılan naproksen metabolitlerinin, dikkate alınmayan yan etkilerinden kaçınmak için; naproksen metabolitlerinin birikimini belirlemek amacı ile daha ileri çalışmalar yapılmalıdır.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: O-desmethylnaproxen Production and Isolationmentioning

confidence: 93%

See 1 more Smart Citation

In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A Mouse Connective Tissue Fibroblast Cells

Akbay

Aracagök

Göker

et al. 2021

Hacettepe Journal of Biology and Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Some species of Penicillium can be used effectively in TCS, NP and hormones degradation [198][199][200][201][202]. The yeast Yarrowia lipolytica has been studied for the expression of cytochrome P450 and it seems capable of degrading diclofenac and NPX [191,203]. Trichoderma harzianum is able to degrade 17 β-estradiol [204] and CBZ with a performance similar to that of Pleurotus ostreatus [205].…”

Section: Mycoremediationmentioning

confidence: 99%

From Laboratory Tests to the Ecoremedial System: The Importance of Microorganisms in the Recovery of PPCPs-Disturbed Ecosystems

et al. 2020

View full text Add to dashboard Cite

The presence of a wide variety of emerging pollutants in natural water resources is an important global water quality challenge. Pharmaceuticals and personal care products (PPCPs) are known as emerging contaminants, widely used by modern society. This objective ensures availability and sustainable management of water and sanitation for all, according to the 2030 Agenda. Wastewater treatment plants (WWTP) do not always mitigate the presence of these emerging contaminants in effluents discharged into the environment, although the removal efficiency of WWTP varies based on the techniques used. This main subject is framed within a broader environmental paradigm, such as the transition to a circular economy. The research and innovation within the WWTP will play a key role in improving the water resource management and its surrounding industrial and natural ecosystems. Even though bioremediation is a green technology, its integration into the bio-economy strategy, which improves the quality of the environment, is surprisingly rare if we compare to other corrective techniques (physical and chemical). This work carries out a bibliographic review, since the beginning of the 21st century, on the biological remediation of some PPCPs, focusing on organisms (or their by-products) used at the scale of laboratory or scale-up. PPCPs have been selected on the basics of their occurrence in water resources. The data reveal that, despite the advantages that are associated with bioremediation, it is not the first option in the case of the recovery of systems contaminated with PPCPs. The results also show that fungi and bacteria are the most frequently studied microorganisms, with the latter being more easily implanted in complex biotechnological systems (78% of bacterial manuscripts vs. 40% fungi). A total of 52 works has been published while using microalgae and only in 7% of them, these organisms were used on a large scale. Special emphasis is made on the advantages that are provided by biotechnological systems in series, as well as on the need for eco-toxicological control that is associated with any process of recovery of contaminated systems.

show abstract

“…For this reason, an alternative might be the enzymatic oxidation. It was observed that the selected fungal strains can effectively degrade selected pharmaceuticals (Aracagök et al 2018). Fungal enzymes also appear to be particularly promising in this issue, for example heme-thiolate peroxidase, which is unspecific peroxygenase extracted from the Black poplar mushroom Agrocybe aegerita (AaeUPO EC 1.11.2.1) discovered over decade ago (Ullrich 2004).…”

Section: Introductionmentioning

confidence: 99%

Archives of Environmental Protection

Lemańska,

Felis,

Poraj-Kobielska

et al. 2021

View full text Add to dashboard Cite

Sulphonamides (SAs) are one of the most frequently detected anthropogenic micropollutants in the aquatic environment and their presence in it may pose a threat to living organisms. The aim of the study was to determine susceptibility of selected sulphonamides, i.e. sulfadiazine (SDZ) and sulfamethazine (SMZ), to degradation in the ozonation process and in enzymatic oxidation by unspecific peroxygenase extracted from Agrocybe aegerita mushroom (AaeUPO). Moreover, the acute toxicity of the aqueous solution of the selected sulphonamides (SMZ and SDZ) before and after mentioned treatment processes were studied on the freshwater crustacean Daphnia magna. Initial concentrations were equal to 2×10 -5 M for sulfadiazine and 1.8×10 -5 M for sulfamethazine. The percentage of transformation for the O 3 process was at the level 95% for both SDZ and SMZ (after 10 s of the process), whilst enzymatic oxidation of SDZ and SMZ by AaeUPO caused transformation efficiencies at the levels of 97% and 94% (after 1 minute of the process), respectively. The second order rate constants of selected sulfonamides with molecular ozone and fungal peroxidase were also determined in the research. EC 50 (median effective concentration) values from toxicity test on D. magna were found in the range from 14.6% to 37.2%, depending on the type of the process. The conducted oxidation processes were efficient in degradation of selected sulphonamides. The toxicity of the mixtures before and after treatment was comparable and did not change significantly. The research have shown that biological processes are not always safer for living organisms compared to the chemical processes.

show abstract

Biodegradation of micropollutant naproxen with a selected fungal strain and identification of metabolites

Cited by 27 publications

References 38 publications

In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A Mouse Connective Tissue Fibroblast Cells

In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A Mouse Connective Tissue Fibroblast Cells

From Laboratory Tests to the Ecoremedial System: The Importance of Microorganisms in the Recovery of PPCPs-Disturbed Ecosystems

Archives of Environmental Protection

Contact Info

Product

Resources

About