Biodegradation of micro-polyethylene particles by bacterial colonization of a mixed microbial consortium isolated from a landfill site

Park, Seon Yeong; Kim, Chang Gyun

doi:10.1016/j.chemosphere.2019.01.159

Cited by 274 publications

(85 citation statements)

References 19 publications

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“…) into the inert carbon backbone (Albertsson et al, 1987;Orr et al, 2004;Hadad et al, 2005). The complex process of complete biodegradation of polyethylene follows these steps: (a) fragmentation by microbial intervention (adhering to the surface of polyethylene) or environmental components and incorporation of functional groups if applicable; (b) breaking down of polymer into oligomers or monomers as well as fatty acids, ketons, aldehydes, alcohols, etc., by enzymatic attack and free radicals; (c) uptake of these small products inside the microbial cells; (d) utilization of those molecules in cellular metabolism and finally production of CO 2 , N 2 , CH 4 , H 2 O, etc., (Yang et al, 2014;Wilkes and Aristilde, 2017;Muhonja et al, 2018;Delacuvellerie et al, 2019;Park and Kim, 2019). Both culture-based and culture-independent metagenomic studies have highlighted the PE (LDPE or HDPE) biodegradation abilities of several bacterial taxa viz.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Unpretreated Low-Density Polyethylene (LDPE) by Stenotrophomonas sp. and Achromobacter sp., Isolated From Waste Dumpsite and Drilling Fluid

et al. 2020

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Exploring the catabolic repertoire of natural bacteria for biodegradation of plastics is one of the priority areas of biotechnology research. Low Density Polyethylene (LDPE) is recalcitrant and poses serious threats to our environment. The present study explored the LDPE biodegradation potential of aerobic bacteria enriched from municipal waste dumpsite and bentonite based drilling fluids from a deep subsurface drilling operation. Considerable bacterial growth coupled with significant weight loss of the LDPE beads (∼8%), change in pH to acidic condition and biofilm cell growth around the beads (CFU count 105–106/cm2) were noted for two samples (P and DF2). The enriched microbial consortia thus obtained displayed high (65–90%) cell surface hydrophobicity, confirming their potential toward LDPE adhesion as well as biofilm formation. Two LDPE degrading bacterial strains affiliated to Stenotrophomonas sp. and Achromobacter sp. were isolated as pure culture from P and DF2 enrichments. 16S rRNA gene sequences of these isolates indicated their taxonomic novelty. Further biodegradation studies provided strong evidence toward the LDPE metabolizing ability of these two organisms. Atomic Fore Microscopy (AFM) and Scanning Electron Microscopy (SEM) revealed considerable damage (in terms of formation of cracks, grooves, etc.) on the micrometric surface of the LDPE film. Analysis of the average roughness (Ra), root mean square roughness (Rq), average height (Rz), maximum peak height (Rp), and maximum valley depth (Rv) (nano-roughness parameters) through AFM indicated 2–3 fold increase in nano-roughness of the LDPE film. FTIR analysis suggested incorporation of alkoxy (1000–1090 cm–1), acyl (1220 cm–1), nitro (1500–1600 cm–1), carbonyl (1720 cm–1) groups into the carbon backbone, formation of N-O stretching (1360 cm–1) and chain scission (905 cm–1) in the microbially treated LDPEs. Increase in carbonyl index (15–20 fold), double bond index (1.5–2 fold) and terminal double bond index (30–40 fold) confirmed that biodegraded LDPEs had undergone oxidation, vinylene formation and chain scission. The data suggested that oxidation and dehydrogenation could be the key steps allowing formation of low molecular weight products suitable for their further mineralization by the test bacteria. The study highlighted LDPE degrading ability of natural bacteria and provided the opportunity for their development in plastic remediation process.

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Section: Introductionmentioning

confidence: 99%

Biodegradation of Unpretreated Low-Density Polyethylene (LDPE) by Stenotrophomonas sp. and Achromobacter sp., Isolated From Waste Dumpsite and Drilling Fluid

et al. 2020

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“…One potential option is to look for organisms or other substances that can degrade MNPs. Marine fungus Zalerion maritimum and mixed bacteria have been found to have positive and effective effects on plastic degradation [118,119]. In addition, a recent study reported that PET polyester plastics could be rapidly and efficiently degraded by special enzymes.…”

Section: Feasible Control or Disposal Measures Of Mnpsmentioning

confidence: 99%

Health impacts of environmental contamination of micro- and nanoplastics: a review

Jiang

Kauffman

et al. 2020

Environ Health Prev Med

222

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Plastics are extensively used in our daily life. However, a significant amount of plastic waste is discharged to the environment directly or via improper reuse or recycling. Degradation of plastic waste generates micro-or nanosized plastic particles that are defined as micro-or nanoplastics (MNPs). Microplastics (MPs) are plastic particles with a diameter less than 5 mm, while nanoplastics (NPs) range in diameter from 1 to 100 or 1000 nm. In the current review, we first briefly summarized the environmental contamination of MNPs and then discussed their health impacts based on existing MNP research. Our review indicates that MNPs can be detected in both marine and terrestrial ecosystems worldwide and be ingested and accumulated by animals along the food chain. Evidence has suggested the harmful health impacts of MNPs on marine and freshwater animals. Recent studies found MPs in human stool samples, suggesting that humans are exposed to MPs through food and/or drinking water. However, the effect of MNPs on human health is scarcely researched. In addition to the MNPs themselves, these tiny plastic particles can release plastic additives and/or adsorb other environmental chemicals, many of which have been shown to exhibit endocrine disrupting and other toxic effects. In summary, we conclude that more studies are necessary to provide a comprehensive understanding of MNP pollution hazards and also provide a basis for the subsequent pollution management and control.

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“…These significant ecological impacts persuade the researchers to investigate the aging behavior of these tiny particles in an environment. Till date, several studies have been conducted to determine the changes in physicochemical properties occurred as a result of physical, chemical [13,14] and biological processes [14], moreover the requirement for the detailed research is obligatory.…”

Section: Introductionmentioning

confidence: 99%

Surface modification of polyethylene microplastic particles during the aqueous-phase ozonation process

Zafar

Park

Kim

2020

Environmental Engineering Research

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Microplastics coexist with the chemical reactive oxygen species in natural waters, however, there is still a lack to elucidate the effect of these radicals on the microplastic surficial oxidation. In this study, the ozonation of polyethylene microplastics was carried out under varying ozone dosages ranging from 4 to 7 mg/min for 60, 120 and 180 min, where its ozone uptake was iodometrically compared and surficial modification was spectroscopically analyzed using FTIR and XPS. For that, the lowest ozone uptake was 16% at 4 mg/min ozone supplied for 60 min whereas the highest was observed of 44% at 7 mg/min ozone added for 180 min. Moreover, in the FTIR analysis, carbonyl (1,600-1,800 cm-1) and hydroxyl (3,200-3,600 cm-1) indices were improved more than 20% and 13% when they were ozonized at 7 mg/min for 180 min compared to 4 mg/min for 60 min, respectively. XPS also revealed that 7 mg/min of ozone supplied for 180 min provided the highest of oxygen functionalities, but while there was no significant change in CC bond. It can be concluded that the surficial modification of PE including formation of oxygen functionalities could be more preferably influenced by the reaction time than ozone dosages.

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Biodegradation of micro-polyethylene particles by bacterial colonization of a mixed microbial consortium isolated from a landfill site

Cited by 274 publications

References 19 publications

Biodegradation of Unpretreated Low-Density Polyethylene (LDPE) by Stenotrophomonas sp. and Achromobacter sp., Isolated From Waste Dumpsite and Drilling Fluid

Biodegradation of Unpretreated Low-Density Polyethylene (LDPE) by Stenotrophomonas sp. and Achromobacter sp., Isolated From Waste Dumpsite and Drilling Fluid

Health impacts of environmental contamination of micro- and nanoplastics: a review

Surface modification of polyethylene microplastic particles during the aqueous-phase ozonation process

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