Biodegradation of methyl tert-butyl ether by newly identified soil microorganisms in a simple mineral solution

Barberà, Maria José; Mateo, Estíbaliz; Monkaityte, Rasa; Constantí, Magda

doi:10.1007/s11274-010-0522-4

Cited by 11 publications

(6 citation statements)

References 36 publications

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“…Bacillus strains were also cultivated from oxygenate-contaminated sources in this study (Fig. 6a), as well as Kao et al (2010) and Barbera et al (2011); however, this may be due to spore resistance and longevity in groundwater.…”

Section: Resultsmentioning

confidence: 87%

Biological and chemical analyses of a laboratory-scale biofilter for oxygenate bioremediation in simulated groundwater

Purswani

Silva-Castro

Guisado

et al. 2013

Int. J. Environ. Sci. Technol.

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Microbial remediation of methyl tert-butyl ether-contaminated aquifers has been widely studied since their cost/efficiency ratios are lower than other remediating techniques. Based on previous studies, simultaneous assessment of two inocula (Acinetobacter calcoaceticus M10 and a co-culture between strain M10 and Rhodococcus ruber E10) was performed for methyl tert-butyl ether bioremediation in a designed pilot-scale biofilter. A noninoculated biofilter was included for comparison. Temporary bioremediation of methyl tert-butyl ether (up to 80 % removal) from the biofilter inoculated with the consortium was observed within 44 days. In addition, the taxonomic profile (temporal temperature gradient gel electrophoresis from RNA extracts) from the latter biofilter contained more active strains than in the other two systems. The presence of strains M10 and E10 in temporal temperature gradient gel electrophoresis analysis from RNA extracts and high presence of strain E10 in temporal temperature gradient gel electrophoresis analysis of DNA extracts, along with alkB amplification of both strains in the biofilter, suggest that the co-culture inoculum was responsible for the methyl tertbutyl ether removal.

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Section: Resultsmentioning

confidence: 87%

Biological and chemical analyses of a laboratory-scale biofilter for oxygenate bioremediation in simulated groundwater

Purswani

Silva-Castro

Guisado

et al. 2013

Int. J. Environ. Sci. Technol.

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“…Jose Barbera et al confirmed that E. cloacae species play an important role in the MTBE biodegradation. They also showed that E. cloacae MCM2/1 has a high potential for utilizing MTBE (39). In the first hours, degradation rate by K. planticola was higher than other strains, and it seems that E. coli needs more time for degrading MTBE (Figure 3), it can be due to the complex molecular structure of MTBE, while the cultures containing yeast extract, this compound makes a good condition for better growth of the strains (19).…”

Section: Discussionmentioning

confidence: 99%

Bioremediation of methyl tertiary-butyl ether (MTBE) by three pure bacterial cultures

Yousefi¹,

Tahernezhad²,

Mousavinasab³

et al. 2018

Environ Health Eng Manag

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Background: Bioremediation of groundwater and soil contamination is more economical than physicochemical remediation. The present study focused on the bioremediation capability of two bacterial species (Klebsiella planticola and Enterobacter cloacae) from the family Enterobacteriaceae. These bacteria have been identified as new species with capability of degrading methyl tertiary-butyl ether (MTBE). In order to enhance their degradation capability, selected concentrations and retention time were investigated. Methods: The bacteria were cultured on the nutrient agar (NA) medium at room temperature. pH of the medium was adjusted to 7. The medium was autoclaved at 121°C for 15 minutes and incubated for 24 hours at 35°C. After 24 hours, the mixture was inoculated into 50 mL of Luria Bertani (LB) liquid medium containing 50 and 150 ppm MTBE. The cultures were incubated for 2 and 5 days at 35°C and shacked on a shaker at 150 rpm. Cell concentrations of the bacteria in pure culture were determined from the optical density at 600 nm using a UV-VIS spectrophotometer. Then, the culture was centrifuged at 3800 rpm for 20 minutes. In the next step, the MTBE concentration in the supernatant was measured by gas chromatography/mass spectrometry (GC/MS, Agilent Technologies, 5975, US10304411, 5.02.07). Results:The results showed that both strains are able to grow in the presence of 50 and 150 ppm MTBE. In the best conditions, when cell density was 3×108 CFU/mL during 5 days, the highest rate of MTBE degradation for K. planticola and E. cloacae, was 43% and 40%, respectively. It was also revealed that Escherichia coli can degrade 50 and 150 ppm MTBE about 19.8% and 13.65%, respectively. Conclusion: It seems that E. coli can be a good candidate for MTBE degradation at high concentrations for a time longer than that in the present study. It was also found that the species have high performance at 50 ppm than 150 ppm. So, these bacteria can remove MTBE from the environment.

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“…The metabolic flexibility of these genera has been described in several studies, where degradation of organic pollutants was demonstrated (Ghosal et al, 2010;Ryan et al, 2009, Zhang et al, 2007. Some studies describe the complete aerobic degradation of MTBE (Barberà et al, 2011;Nipkey & Nipkey, 2006), but not its anaerobic degradation, because the accumulation of toxic tert-butyl alcohol may occur (Wei & Finneran, 2011).…”

Section: Discussionmentioning

confidence: 99%

Coupling Flow Analysis With Geochemical and Microbiological Analyses to Assess the Bioremediation Feasibility of a Contaminated Aquifer

Gigliuto¹,

Stopelli

Sanangelantoni³

et al. 2016

Remediation

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An integrated approach combining classic and molecular microbiological methods, “in vitro” bioremediation assays and groundwater numerical modeling, has been established to identify optimized solutions for remediating aquifers contaminated with organic pollutants. Bacteria have been isolated from an aquifer contaminated with toluene and methyl tert‐butyl ether (MTBE), selected for their growth with contaminants as a sole carbon source and identified through 16S rDNA partial sequencing. Successive biodegradation laboratory tests have been performed to determine which chemical conditions were more appropriate for the isolated bacteria to more efficiently oxidize toluene and MTBE. A groundwater model was created using FEFLOW code first to determine the movement of the plume front and second to simulate the impact of the biodegradation processes along the groundwater flow directions based on the bioremediation rates obtained in the laboratory. The results show that this innovative and interdisciplinary model can be used to assist in developing monitoring and remediation plans for cleaning up complex contaminated groundwater sites. This approach successfully combines the identification of the optimum biogeochemical conditions for bacterial biodegradation to occur with the predictability of the development of the process over time, ensuring decisive support in the management of contaminated sites. ©2016 Wiley Periodicals, Inc.

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Biodegradation of methyl tert-butyl ether by newly identified soil microorganisms in a simple mineral solution

Cited by 11 publications

References 36 publications

Biological and chemical analyses of a laboratory-scale biofilter for oxygenate bioremediation in simulated groundwater

Biological and chemical analyses of a laboratory-scale biofilter for oxygenate bioremediation in simulated groundwater

Bioremediation of methyl tertiary-butyl ether (MTBE) by three pure bacterial cultures

Coupling Flow Analysis With Geochemical and Microbiological Analyses to Assess the Bioremediation Feasibility of a Contaminated Aquifer

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