Biodegradation of methyl tert-butyl ether by cold-adapted mixed and pure bacterial cultures

Zaitsev, Gennadi; Uotila, Jussi; Häggblom, Max M.

doi:10.1007/s00253-006-0737-3

Cited by 38 publications

(24 citation statements)

References 29 publications

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“…Experimental yields of ¢0.5 g g 21 prove the effective use of these substrates for the growth and multiplication of strain L108. A similar yield coefficient of 0.49 g g 21 was obtained with Variovorax paradoxus CL-8 (Zaitsev et al, 2007). For Mycobacterium austroafricanum IFP2012, yields of 0.44 g g 21 (MTBE), 0.61 g g 21 (TBA) and 0.42 g g 21 (2-HIBA) were reported (François et al, 2002).…”

supporting

confidence: 55%

“…A mixed culture with V. paradoxus CL-8 as the MTBE-degrading entity exhibited a growth rate of 0.012 h 21 (Zaitsev et al, 2007); a rate even one magnitude lower was reported for another mixed culture (Lin et al, 2007). Mycobacterium austroafricanum IFP2012 (François et al, 2002) and IFP2015 (François et al, 2003), Hydrogenophaga flava ENV735 (Hatzinger et al, 2001) and Methylibium petroleiphilum PM1 (Hanson et al, 1999) appeared to utilize MTBE in batch degradation experiments, but specific rates were hardly accessible or even impossible to derive from the published data and indicated difficulties in sustaining growth.…”

Section: Introductionmentioning

confidence: 99%

“…These include Methylibium petroleiphilum PM1 (Nakatsu et al, 2006), Methylibium sp. R8 (Rosell et al, 2007), Hydrogenophaga flava ENV735 (Hatzinger et al, 2001), Mycobacterium austroafricanum IFP2012 and IFP2015 (François et al, 2002(François et al, , 2003Lopes Ferreira et al, 2006), Variovorax paradoxus CL-8 (Zaitsev et al, 2007), and other strains described in little detail (Hernandez-Perez et al, 2001;Lin et al, 2007;Okeke & Frankenberger, 2003;Pruden & Suidan, 2004). In addition, we have recently isolated strain L108 from a polluted site in Germany, which is able to grow on MTBE, ETBE and TAME (Rohwerder et al, 2004(Rohwerder et al, , 2006 and, like Methylibium petroleiphilum PM1, belongs to the Ideonella-Leptothrix-Rubrivivax branch of the b-proteobacteria.…”

Section: Introductionmentioning

confidence: 99%

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Degradation of fuel oxygenates and their main intermediates by Aquincola tertiaricarbonis L108

2008

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Growth of Aquincola tertiaricarbonis L108 on the fuel oxygenates methyl tert-butyl ether (MTBE), ethyl tert-butyl ether (ETBE) and tert-amyl methyl ether (TAME), as well as on their main metabolites tert-butyl alcohol (TBA), tert-amyl alcohol (TAA) and 2-hydroxyisobutyrate (2-HIBA) was systematically investigated to characterize the range and rates of oxygenate degradation by this strain. The effective maximum growth rates for MTBE, ETBE and TAME at pH 7 and 30 6C were 0.045 h "1 , 0.06 h "1 and 0.055 h "1, respectively, whereas TAA, TBA and 2-HIBA permitted growth at rates up to 0.08 h , respectively. The experimental growth yields with all these substrates were high. Yields of 0.55 g dry mass (dm) (g MTBE) "1 , 0.53 g dm (g ETBE) "1 , 0.81 g dm (g TAME) , and 3.27 mmol 2-HIBA h "1 g "1. The relatively high rates with TBA, TAA and 2-HIBA indicate that the transformations of these metabolites did not limit the metabolism of MTBE and the related ether compounds. Despite the fact that these metabolites still carry a tertiary carbon atom that is commonly suspected to confer recalcitrance to the ether oxygenates, the transformation rates were in the same range as those with succinate and fructose. With MTBE, strain L108 grew at pHs between 5.5 and 8.0 at near-maximal rate, whereas no growth was found below pH 5.0 and above pH 9.0. The optimum growth temperature was 30 6C, but at 5 6C still about 15 % of the maximum rate remained, whereas no growth occurred at 42 6C. This indicates that MTBE metabolites are valuable substrates and that A. tertiaricarbonis L108 is a good candidate for bioremediation purposes. The possible origin of its exceptional metabolic capability is discussed in terms of the evolution of enzymic activities involved in the conversion of compounds carrying tertiary butyl groups. INTRODUCTIONMethyl tert-butyl ether (MTBE) and the related compounds ethyl tert-butyl ether (ETBE) and tert-amyl methyl ether (TAME) are widely used as oxygenating compounds in gasoline (Krayer von Krauss & Harremoës, 2001), leading to pollution by unnoticed leakages and accidental spills (Baehr et al., 1999;Klinger et al., 2002; Schmidt et al., 2002;Squillace et al., 1996). They threaten water resources by their unpleasant odour and taste and suspected carcinogenicity (McGregor, 2006). Consequently, much effort is put into studying the environmental fate of these compounds and developing measures against fuel oxygenate pollution. Microbial degradation has been considered in both respects (Deeb et al., 2000;Fayolle et al., 2001;Schmidt et al., 2004). However, MTBE and structurally related compounds were initially found to withstand microbial attack. This was thought to be mainly due to the ether bond in these compounds and the presence of a tertiary carbon atom.Generally, it has proved difficult to isolate strains from enrichment cultures using MTBE as sole carbon and energy source. Attempts for more than 15 years were of limited success. At present, there are only a few strains capable of growing solely on oxygenates. The...

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supporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Degradation of fuel oxygenates and their main intermediates by Aquincola tertiaricarbonis L108

2008

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“…Therefore, this could be an indication of biodegradation, which might still be possible under such cold conditions. Several aerobic MTBE/TBA degraders have shown growth on MTBE in a wide range of temperatures, for example, a cold-adapted mixed culture containing Variovorax paradoxus strain CL-8 (growth range, 3 to 30°C; optimum, 18 to 22°C) (56) or A. tertiaricarbonis L108 (growth range, 4 to 40°C), which had a growth rate of about 15% of its maximum (optimum, 30°C) at 5°C and could even degrade MTBE below this temperature (38). Similar higher MTBE removals in the two aerated treatment ponds due to the increase of oxygen dissolved in the water (period A2) were obtained.…”

Section: Discussionmentioning

confidence: 99%

Linking Low-Level Stable Isotope Fractionation to Expression of the Cytochrome P450 Monooxygenase-Encoding ethB Gene for Elucidation of Methyl tert -Butyl Ether Biodegradation in Aerated Treatment Pond Systems

Jechalke

Rosell

Lavanchy

et al. 2011

Appl Environ Microbiol

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Multidimensional compound-specific stable isotope analysis (CSIA) was applied in combination with RNA-based molecular tools to characterize methyl tertiary (tert-) butyl ether (MTBE) degradation mechanisms occurring in biofilms in an aerated treatment pond used for remediation of MTBE-contaminated groundwater. The main pathway for MTBE oxidation was elucidated by linking the low-level stable isotope fractionation (mean carbon isotopic enrichment factor [ C ] of ؊0.37‰ ؎ 0.05‰ and no significant hydrogen isotopic enrichment factor [ H ]) observed in microcosm experiments to expression of the ethB gene encoding a cytochrome P450 monooxygenase able to catalyze the oxidation of MTBE in biofilm samples both from the microcosms and directly from the ponds. 16S rRNA-specific primers revealed the presence of a sequence 100% identical to that of Methylibium petroleiphilum PM1, a well-characterized MTBE degrader. However, neither expression of the mdpA genes encoding the alkane hydroxylase-like enzyme responsible for MTBE oxidation in this strain nor the related MTBE isotope fractionation pattern produced by PM1 could be detected, suggesting that this enzyme was not active in this system. Additionally, observed low inverse fractionation of carbon ( C of ؉0.11‰ ؎ 0.03‰) and low fractionation of hydrogen ( H of ؊5‰ ؎ 1‰) in laboratory experiments simulating MTBE stripping from an open surface water body suggest that the application of CSIA in field investigations to detect biodegradation may lead to false-negative results when volatilization effects coincide with the activity of low-fractionating enzymes. As shown in this study, complementary examination of expression of specific catabolic genes can be used as additional direct evidence for microbial degradation activity and may overcome this problem.

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“…A fent bemutatott izolátumokon kívül egyéb törzsekről is leírták, hogy az éter típusú üzemanyag-oxigenátok bontására képesek [74,119,[137][138][139][140][141][142][143][144][145] …”

Section: áBra Az Etbe Metabolikus úTvonala a Rhodococcus Ruber Ifp 20unclassified

Éter típusú üzemanyag-adalékok mikrobiális bontása

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Biodegradation of methyl tert-butyl ether by cold-adapted mixed and pure bacterial cultures

Cited by 38 publications

References 29 publications

Degradation of fuel oxygenates and their main intermediates by Aquincola tertiaricarbonis L108

Degradation of fuel oxygenates and their main intermediates by Aquincola tertiaricarbonis L108

Linking Low-Level Stable Isotope Fractionation to Expression of the Cytochrome P450 Monooxygenase-Encoding ethB Gene for Elucidation of Methyl tert -Butyl Ether Biodegradation in Aerated Treatment Pond Systems

Éter típusú üzemanyag-adalékok mikrobiális bontása

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