Biodegradation of Methyl <i>tert</i>-Butyl Ether under Various Substrate Conditions

Pruden, Amy; Suidan, Makram T.; Venosa, and Albert D.; Wilson, Gregory J.

doi:10.1021/es010572z

Cited by 52 publications

(48 citation statements)

References 38 publications

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“…The culture was thawed prior to inoculation. All the aforementioned microbial cultures were previously grown under aerobic conditions and at ambient temperature before being used to inoculate the reactors (Morrison et al 2001;Pruden et al 2001;Zein et al 2004). An acclimation period of 2 weeks was required before efficient biodegradation of the target contaminants was observed in the two reactors.…”

Section: Media and Culture Conditionsmentioning

confidence: 99%

Treatment of Groundwater Contaminated with PAHs, Gasoline Hydrocarbons, and Methyl tert-butyl Ether in a Laboratory Biomass-Retaining Bioreactor

et al. 2006

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In this study, we investigated the treatability of co-mingled groundwater contaminated with polycyclic aromatic hydrocarbons (PAHs), gasoline hydrocarbons, and methyl tert-butyl ether (MtBE) using an ex-situ aerobic biotreatment system. The PAHs of interest were naphthalene, methyl-naphthalene, acenaphthene, acenaphthylene, and carbazole. The gasoline hydrocarbons included benzene, toluene, ethyl benzene, and p-xylene (BTEX). Two porous pot reactors were operated for a period of 10 months under the same influent contaminant concentrations. The contaminated groundwater was introduced into the reactors at a flow rate of 4 and 9 l/day, resulting in a hydraulic retention time (HRT) of 32 and 15 h, respectively. In both reactors, high removal efficiencies were achieved for the PAHs (>99%), BTEX and MtBE (>99.7%). All the PAHs of interest and the four BTEX compounds were detected at concentrations less than 1 lg/l throughout the study duration. Effluent MtBE from both reactors was observed at higher levels; nevertheless, its concentration was lower than the 5 lg/l Drinking Water Advisory for MtBE implemented in California.

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Section: Media and Culture Conditionsmentioning

confidence: 99%

Treatment of Groundwater Contaminated with PAHs, Gasoline Hydrocarbons, and Methyl tert-butyl Ether in a Laboratory Biomass-Retaining Bioreactor

et al. 2006

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“…A mixed culture may be more suitable for treatment options than pure culture (Pruden et al, 2001). It could be the existence of many rea- (Francois et al, 2003;Smith et al, 2003;Ferreira et al, 2006).…”

Section: Isolation Of Mtbe-degrading Bacteria From the Gasoline Contamentioning

confidence: 99%

Evaluation of Intrinsic Bioremediation of Methyl Tert-butyl Ether (MTBE) Contaminated Groundwater

Chen¹,

Tien²,

Zhan³

2014

Journal of Soil and Groundwater Environment

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This paper reported the use of real-time polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), and the culture-based method in the intrinsic bioremediation study at a petroleum contaminated site. The study showed that phenol hydroxylase gene was detected in groundwater contaminated with benzene, toluene, ethylbenzene, xylene isomers (BTEX) and methyl tert-butyl ether (MTBE). This indicated that intrinsic bioremediation occurred at the site. DGGE analyses revealed that the petroleum-hydrocarbon plume caused the variation in microbial communities. MTBE degraders including Pseudomonas sp. NKNU01, Bacillus sp. NKNU01, Klebsiella sp. NKNU01, Enterobacter sp. NKNU01, and Enterobacter sp. NKNU02 were isolated from the contaminated groundwater using the cultured-based method. Among these five strains, Enterobacter sp. NKNU02 is the most effective stain at degrading MTBE without the addition of pentane. The MTBE biodegradation experiment indicated that the isolated bacteria were affected by propane. Biodegradation of MTBE was decreased but not totally inhibited in the mixtures of BTEX. Enterobacter sp. NKNU02 degraded about 60% of MTBE in the bioreactor study. Tert-butyl alcohol (TBA), acetic acid, 2-propanol, and propenoic acid were detected using gas chromatography/mass spectrometry during MTBE degraded by the rest cells of Enterobacter sp. NKNU02. The effectiveness of bioremediation of MTBE was assessed for potential field-scale application.

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“…Further, Sphingomonas spp. have also been associated with aerobic MTBE biodegradation in bioreactors (43,70). Recently, a 16S rRNA gene sequence classifying as a Sphingomonas sp.…”

Section: Figmentioning

confidence: 99%

Anaerobic Methyl tert -Butyl Ether-Degrading Microorganisms Identified in Wastewater Treatment Plant Samples by Stable Isotope Probing

Sun

Cupples

2012

Appl Environ Microbiol

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ABSTRACTAnaerobic methyltert-butyl ether (MTBE) degradation potential was investigated in samples from a range of sources. From these 22 experimental variations, only one source (from wastewater treatment plant samples) exhibited MTBE degradation. These microcosms were methanogenic and were subjected to DNA-based stable isotope probing (SIP) targeted to both bacteria and archaea to identify the putative MTBE degraders. For this purpose, DNA was extracted at two time points, subjected to ultracentrifugation, fractioning, and terminal restriction fragment length polymorphism (TRFLP). In addition, bacterial and archaeal 16S rRNA gene clone libraries were constructed. The SIP experiments indicated bacteria in the phylaFirmicutes(familyRuminococcaceae) andAlphaproteobacteria(genusSphingopyxis) were the dominant MTBE degraders. Previous studies have suggested a role forFirmicutesin anaerobic MTBE degradation; however, the putative MTBE-degrading microorganism in the current study is a novel MTBE-degrading phylotype within this phylum. Two archaeal phylotypes (generaMethanosarcinaandMethanocorpusculum) were also enriched in the heavy fractions, and these organisms may be responsible for minor amounts of MTBE degradation or for the uptake of metabolites released from the primary MTBE degraders. Currently, limited information exists on the microorganisms able to degrade MTBE under anaerobic conditions. This work represents the first application of DNA-based SIP to identify anaerobic MTBE-degrading microorganisms in laboratory microcosms and therefore provides a valuable set of data to definitively link identity with anaerobic MTBE degradation.

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Biodegradation of Methyl tert-Butyl Ether under Various Substrate Conditions

Cited by 52 publications

References 38 publications

Treatment of Groundwater Contaminated with PAHs, Gasoline Hydrocarbons, and Methyl tert-butyl Ether in a Laboratory Biomass-Retaining Bioreactor

Treatment of Groundwater Contaminated with PAHs, Gasoline Hydrocarbons, and Methyl tert-butyl Ether in a Laboratory Biomass-Retaining Bioreactor

Evaluation of Intrinsic Bioremediation of Methyl Tert-butyl Ether (MTBE) Contaminated Groundwater

Anaerobic Methyl tert -Butyl Ether-Degrading Microorganisms Identified in Wastewater Treatment Plant Samples by Stable Isotope Probing

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