Biodegradation of MC252 polycyclic aromatic hydrocarbons and alkanes in two coastal wetlands

Rodrigue, Matthew; Elango, Vijaikrishnah; Curtis, David; Collins, Autumn W.; Pardue, John H.

doi:10.1016/j.marpolbul.2020.111319

Cited by 10 publications

(4 citation statements)

References 54 publications

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“…In contrast, soil prokaryote communities shifted in the soil in response to oil addition, lending support to a relationship that has been demonstrated in field and mesocosm work (5,6,34,54). Composition was differentiated further by the interaction of the oil with different inocula, suggesting that the response of a soil community to the addition of oil will be somewhat unique and depend on characteristics of the soil, like its oiling history.…”

Section: Microbial Community Composition With Respect To Plant Compar...supporting

confidence: 60%

“…Plant presence clearly enhanced the decomposition of PAHs over the course of two years. The relative decrease in two-and three-ring PAHs to four-ring PAHs (chrysenes) suggest biodegradation of PAHs (34). Biodegradation of recalcitrant oil residues in coastal marshes is likely driven by a complex concert of oxygen availability and chemical cues driving changes in microbial communities (35-37).…”

Section: Effect Of Plant Presence and Inoculum On Pah Compositionmentioning

confidence: 99%

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Decay of oil residues in the soil is enhanced by the presence of Spartina alterniflora, with no additional effect from microbiome manipulation

Formel¹,

Martin²,

Pardue³

et al. 2022

Front. Soil Sci.

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Recent work has suggested that the phytoremediation potential of S. alterniflora may be linked to a selection by the plant for oil-degrading microbial communities in the soil, in combination with enhanced delivery of oxygen and plant enzymes to the soil. In salt marshes, where the soil is saline and hypoxic, this relationship may be enhanced as plants in extreme environments have been found to be especially dependent on their microbiome for resilience to stress and to respond to toxins in the soil. Optimizing methods for restoration of oiled salt marshes would be especially meaningful in the Gulf of Mexico, where there is a persistently high threat of petroleum contamination. One favorable strategy for restoration of oiled sites might include planting S. alterniflora with a microbiome that has been pre-selected for an oiled environment. We examined this strategy with a two-year greenhouse experiment and found that planting S. alterniflora in an oiled environment enhances decomposition of oil residues without a clear negative impact on plant growth. Manipulation of the S. alterniflora microbiome through soil inoculation and through exposure to oil residues, elicited a detectable response of soil prokaryote communities to the presence of oil, while only demonstrating an analogous response in fungal communities in the plant roots and leaves. Yet, manipulation of the plant microbiome did not change plant morphology or relative decomposition of polycyclic aromatic hydrocarbons (PAHs) in the soil. Therefore, despite evident relationships between the plant, microbes, and oil, manipulation of the microbiome may not be a worthwhile addition to S. alterniflora phytoremediation strategies. Nevertheless, our work adds support for replanting S. alterniflora in oiled salt marshes as an effective strategy for reducing oil residues in salt marshes, in addition to the re-vegetation and erosion reduction benefits demonstrated by others.

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Section: Microbial Community Composition With Respect To Plant Compar...supporting

confidence: 60%

Section: Effect Of Plant Presence and Inoculum On Pah Compositionmentioning

confidence: 99%

Decay of oil residues in the soil is enhanced by the presence of Spartina alterniflora, with no additional effect from microbiome manipulation

Formel¹,

Martin²,

Pardue³

et al. 2022

Front. Soil Sci.

Self Cite

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“…Another striking difference between the incubations was the increase in abundances of sulfur-oxidizing bacteria in crude oil incubations such as members of the genera Halothiobacillus and Sulfuritalea indicating their potential role in biodegrading sulfur components (Li et al 2014 ; Sperfeld et al 2019 ; Rodrigue et al 2020 ; Wei et al 2020 ) whose concentration in the Bonny crude oil is from 0.1 to 0.2% (Nuhu et al 2022 ). Biodegradation of both alkanes and PAHs in the synthetic oil was slower than in the natural oil.…”

Section: Discussionmentioning

confidence: 99%

Correlating the succession of microbial communities from Nigerian soils to petroleum biodegradation

Iturbe-Espinoza

Bonte

Weedon

et al. 2023

World J Microbiol Biotechnol

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Whilst biodegradation of different hydrocarbon components has been widely demonstrated to occur by specialist oil-degrading bacteria, less is known about the impact on microbial communities as a function of oil composition by comparing the biodegradation of chemically complex fuels to synthetic products. The objectives of this study were (i) to assess the biodegradation capacity and succession of microbial communities isolated from Nigerian soils in media with crude oil or synthetic oil as sole sources of carbon and energy, and (ii) to assess the temporal variability of the microbial community size. Community profiling was done using 16 S rRNA gene amplicon sequencing (Illumina), and oil profiling using gas chromatography. The biodegradation of natural and synthetic oil differed probably due to the content of sulfur that may interfere with the biodegradation of hydrocarbons. Both alkanes and PAHs in the natural oil were biodegraded faster than in the synthetic oil. Variable community responses were observed during the degradation of alkanes and more simple aromatic compounds, but at later phases of growth they became more homogeneous. The degradation capacity and the size of the community from the more-contaminated soil were higher than those from the less-contaminated soil. Six abundant organisms isolated from the cultures were found to biodegrade oil molecules in pure cultures. Ultimately, this knowledge may contribute to a better understanding of how to improve the biodegradation of crude oil by optimizing culturing conditions through inoculation or bioaugmentation of specific bacteria during ex-situ biodegradation such as biodigesters or landfarming.

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“…Marinobacter, Alcanivorax, and Idiomarina were still present as the dominant species. Alcanivorax and Idiomarina have been shown to be associated with alkane degradation (Rodrigue et al 2020), while Marinobacter is associated with the degradation of aromatic hydrocarbons (Murphy et al 2021). Furthermore, it was observed that the abundance of Pseudomonas increased significantly, but the relative abundance was below 50% at all sampling sites.…”

Section: Response Of Microbial Communities To Crude Oil Pollutionmentioning

confidence: 99%

Bioaugmentation enhance the bioremediation of marine crude oil pollution: Microbial communities and metabolic pathways

Gao

Cai

Shi

et al. 2022

Water Science and Technology

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Bioaugmentation is an effective strategy used to speed up the bioremediation of marine oil spills. In the present study, a highly efficient petroleum degrading bacterium (Pseudomonas aeruginosa ZS1) was applied to the bioremediation of simulated crude oil pollution in different sampling sites in the South China Sea. The metabolic pathways of ZS1 to degrade crude oil, the temporal dynamics of the microbial community response to crude oil contamination, and the biofortification process were investigated. The results showed that the abundance and diversity of the microbial community decreased sharply after the occurrence of crude oil contamination. The best degradation rate of crude oil, which was achieved in the samples from the sampling site N3 after the addition of ZS1 bacteria, was 50.94% at 50 d. C13 alkanes were totally oxidized by ZS1 in the 50 days. The degradation rate of solid n-alkanes (C18-C20) was about 70%. Based on the whole genome sequencing and the metabolites analysis of ZS1, we found that ZS1 degraded n-alkanes through the terminal oxidation pathway and aromatic compounds through the catechol pathway. This study provides data support for further research on biodegradation pathways of crude oil and contributes to the subsequent development of more reasonable bioremediation strategies.

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Biodegradation of MC252 polycyclic aromatic hydrocarbons and alkanes in two coastal wetlands

Cited by 10 publications

References 54 publications

Decay of oil residues in the soil is enhanced by the presence of Spartina alterniflora, with no additional effect from microbiome manipulation

Decay of oil residues in the soil is enhanced by the presence of Spartina alterniflora, with no additional effect from microbiome manipulation

Correlating the succession of microbial communities from Nigerian soils to petroleum biodegradation

Bioaugmentation enhance the bioremediation of marine crude oil pollution: Microbial communities and metabolic pathways

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