Biodegradation of Malachite Green by Brevibacillus laterosporus MTCC 2298

Abstract

Brevibacillus laterosporus MTCC 2298 was screened for the decolorization of eight triphenylmethane dyes. Decolorization of malachite green was found to be fastest (87% within 3 hours, at the concentration 0.1 g/L) among the screened dyes. Various triphenylmethane dyes showed differential induction patterns of the dye-degrading enzymes. The activities of the laccase, nicotinamide adenine dinucleotidedichlorophenolindophenol reductase (NADH-DCIP reductase), malachite green reductase, and aminopyrine N-demethylase were increased in the cell-free extract obtained after decolorization of malachite green. Fourier transform infrared spectral analysis indicated formation of N-demethylated products, including primary and secondary aryl amines. High-performance liquid chromatography analysis confirmed the transformation of malachite green into new metabolites rather than its reduced form, leucomalachite green. Gas chromatography-mass spectroscopy analysis detected new degradation products, such as reduced tetradesmethyl leucomalachite green (m/z 283) and [4-(1-cyclohexyl)-(1'-phenyl)-methyl]-2, 4-hexenoic acid (m/z 282). Complete decolorization of malachite green also was observed by the partially purified laccase from B. laterosporus. Water Environ. Res., 81, 2329Res., 81, (2009.

Effect of Various Carbon and Nitrogen Sources on Decolorization of Textile Dyes by Pseudomonas taiwanensis Strain TNZ3

“…Additionally, studies have demonstrated that media containing yeast extract worked best for decolorizing Rancid Fast Blue dye [37]. The current investigation confirms the findings of Gomare et al [36] earlier work in that peptone and other inorganic nitrogen sources did not improve color removal by bacterial species (2009). With the addition of yeast extract, a wide spectrum of dyes had a higher percentage of their color removed [38].…”

“…Due to dyes' toxicity to microorganisms, previous research found that low concentrations provided higher color removal and that decolorization % declined at high dye concentrations [36].…”

Preparation of a silver ferrite-assisted tungsten oxide nanocomposite for efficient sonocatalytic degradation of organic pollutants activated by PMS followed by toxicity evaluation

“…The peaks of m/z = 416 and m/z = 387 are assigned to the de-ethylation intermediates of RhB and that of m/z = 442 is assigned to the sulfonation of Mal.G. 68,69 The peak of m/z = 320 is from the intermediate product of m/z = 387. 68 Moreover, the peak of m/z = 320 is due to the fragmentation of m/z = 442 in the Mal.G pathway.…”

Dye degradation by early colonizing marine bacteria from the Arabian Sea, India

“…(Roy et al 2020), 20 mg L -1 using Tenacibaculum sp. (Qu et al 2018), 50 mg L -1 using Kocuria rosea (Parshetti et al 2006), 100 mg L -1 using Brevibacillus laterosporus (Gomare et al 2009), 200 mg L -1 using Pseudomonas putida (Sneha et al 2014) and 400 mg L -1 using Bacillus vietnamensis (Kabeer et al 2019). In the present study, it was observed that the degradation of MG decreased after increasing the dye concentration (>800 mg L -1 ).…”