Biodegradation of Low Density Polythene (LDPE) by Pseudomonas Species

Kyaw, Bhone Myint; Champakalakshmi, Ravi; Sakharkar, Meena Kishore; Lim, Chu Sing; Sakharkar, Kishore R.

doi:10.1007/s12088-012-0250-6

Cited by 261 publications

(116 citation statements)

References 10 publications

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“…The disposal of these used plastic materials by using chemical and physical methods are very expensive and produces persistent organic pollutants (POP’s) known as furans and dioxins, which are reported to be toxic irritant products, resulting into infertility of soil, preventing degradation of the other normal substances, depletion of the underground water source and have proved to be dangerous to animal, human and eco-system10. In addition, plastics are also reported to be biodegraded partially with the help of anaerobic process in composts and soil which produce carbon dioxide, water and methane11.…”

mentioning

confidence: 99%

Evaluation of HDPE and LDPE degradation by fungus, implemented by statistical optimization

Ojha

Pradhan

Singh

et al. 2017

Sci Rep

223

103

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Plastic in any form is a nuisance to the well-being of the environment. The ‘pestilence’ caused by it is mainly due to its non-degradable nature. With the industrial boom and the population explosion, the usage of plastic products has increased. A steady increase has been observed in the use of plastic products, and this has accelerated the pollution. Several attempts have been made to curb the problem at large by resorting to both chemical and biological methods. Chemical methods have only resulted in furthering the pollution by releasing toxic gases into the atmosphere; whereas; biological methods have been found to be eco-friendly however they are not cost effective. This paves the way for the current study where fungal isolates have been used to degrade polyethylene sheets (HDPE, LDPE). Two potential fungal strains, namely, Penicillium oxalicum NS4 (KU559906) and Penicillium chrysogenum NS10 (KU559907) had been isolated and identified to have plastic degrading abilities. Further, the growth medium for the strains was optimized with the help of RSM. The plastic sheets were subjected to treatment with microbial culture for 90 days. The extent of degradation was analyzed by, FE-SEM, AFM and FTIR. Morphological changes in the plastic sheet were determined.

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mentioning

confidence: 99%

Evaluation of HDPE and LDPE degradation by fungus, implemented by statistical optimization

Ojha

Pradhan

Singh

et al. 2017

Sci Rep

223

103

View full text Add to dashboard Cite

show abstract

“…For the accurate measurement of dry weight of residual LDPE, LDPE films were recovered from the degradation medium and they were washed with 2% (v/v) sodium dodecyl sulfate (SDS) solution and further washed with distilled water . The washed LDPE film was dried overnight at 60°C before weighing and the percentage of weight loss was determined using the formula :

Weight loss true(% true) = \frac{Initial weight ‐Final weight}{Initial weight} \times 100

…”

Section: Methodsmentioning

confidence: 99%

“…The extract was evaporated at room temperature and it was mixed with 2 mL chloroform before filtering using 0.2 µm PTFE syringe filter. This filtered sample was injected in GC coupled with MS using helium as carrier gas . In this technique, the mass spectrometer was set to scan over range of one unit.…”

Section: Analytical Proceduresmentioning

confidence: 99%

Microbial degradation of low density polyethylene

Abraham

Ghosh

Mukherjee

et al. 2016

Env Prog and Sustain Energy

100

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Low‐density polyethylene (LDPE) is potential source of environmental pollution. In this study, biodegradation of LDPE was achieved by employing fungus and actinobacteria isolated from waste dumping site. Among all the isolates, two potential strains were obtained through enrichment technique. Based on 18S rRNA and 16S rRNA analyses the isolated strains were identified as Aspergillus nomius and Streptomyces sp., respectively. The biodegradation of LDPE was determined by evaluating weight loss and morphological changes of the LDPE samples. The isolated strains; Aspergillus nomius had the capacity to degrade 4.9% and Streptomyces sp. showed 5.2% of weight loss of LDPE films respectively. Weight loss of LDPE film after inoculation of isolates in degradation medium indicated that it was capable of using polyethylene as carbon and energy source. CO2 evolution was checked after degradation of polyethylene pieces, the level of CO2 reached 2.85 g L−1 in the presence of Aspergillus nomius and Streptomyces sp. produced 4.27 g L−1. Sophisticated instrumentation was used like atomic force microscopy, Fourier transform infra‐red spectroscopy (FTIR). The breakdown products of the LDPE film after exposure to the isolates was recorded employing GCMS. FTIR spectrum of LDPE film confirmed changes in the presence of chemical groups like amine, alkanes, phenols, and alcohol after degradation of LDPE films by Aspergillus nomius and Streptomyces sp. and the most prominent structural changes of the spectrum were observed in the LDPE sample after 90 days of degradation. The results affirmed the isolates were capable of degrading LPDE films efficiently. © 2016 American Institute of Chemical Engineers Environ Prog, 36: 147–154, 2017

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“…After immobilization of ssDNA probe onto the carbon composite electrode, a thin film appeared on the network of c-MWCNT due to the covalent (amide bond) immobilization of ssDNA probe (Fig. 5c) [27] which was changed into a dense surface morphology after hybridization with ssG-DNA of N. meningitidis from patient CSF (Fig. 5d) [28].…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Carbon-Mercaptooctadecane/Carboxylated Multi-walled Carbon Nanotubes Composite Based Genosensor for Detection of Bacterial Meningitis

et al. 2013

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Human brain bacterial meningitis is a lifethreatening disease mainly caused by Neisseria meningitidis, lead to several complications including damage of brain or even death. The present available methods for diagnosis of meningitis have one or more limitations. A rmpM gene based genosensor was fabricated by immobilizing 5 0 -amino modified 19-mer single stranded DNA probe onto carbonmercaptooctadecane/carboxylated multi-walled carbon nanotubes composite electrode and hybridized with 2.5-40 ng/6 lL of single stranded genomic DNA (ssG-DNA) of N. meningitidis from cerebrospinal fluid (CSF) of the suspected meningitis patients. The electrochemical response was measured by using cyclic voltammetry and differential pulse voltammetry (DPV) using 1 mM methylene blue as redox indicator in 30 min (including a response time of 1 min) at 25°C. The sensitivity of the genosensor was 3.762 (lA/cm 2 )/ng and limit of detection was 2 ng of ssG-DNA of N. meningitidis with DPV. The genosensor has specificity only to N. meningitidis and does not hybridize with the genomic DNA of any other possible pathogen in human CSF. The immobilization of the probe and hybridization of the ssG-DNA were characterized by using electrochemical impedance in presence of 5 mM potassium ferricyanide and scanning electron microscopy. The genosensor loses only 12 % of its original DPV current on storage at 4°C for 6 months. Carbon composite based electrochemical array can be constructed to detect multiple bacterial meningitis suspected patient CSF samples during an outbreak of the disease.

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Biodegradation of Low Density Polythene (LDPE) by Pseudomonas Species

Cited by 261 publications

References 10 publications

Evaluation of HDPE and LDPE degradation by fungus, implemented by statistical optimization

Evaluation of HDPE and LDPE degradation by fungus, implemented by statistical optimization

Microbial degradation of low density polyethylene

Carbon-Mercaptooctadecane/Carboxylated Multi-walled Carbon Nanotubes Composite Based Genosensor for Detection of Bacterial Meningitis

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