Biodegradation of Long-Chain n-Paraffins from Waste Oil of Car Engine by Acinetobacter sp.

Abstract

Microorganisms that degrade long-chain n-paraffins from used car engine oil were isolated from soil. For the screening, a fraction of n-paraffin prepared from car engine oil was applied as the sole carbon source. The strain was identified as Acinetobacter sp. The ability of the strain to assimilate long-chain n-paraffins was assessed and characterized. The strain mineralized long-chain n-paraffins (0.1% w/v) in the minimal medium after cultivation for 96 h and also reduced the weight of the waste oil added (1% w/v) by 20% after 72 h without an extracellular biosurfactant. When n-hexadecane was fed as substrate, 1-hexadecanol and 1-hexadecanoic acid were detected as the intermediates by gas chromatography/mass spectrometry. This indicates that the long-chain n-paraffins were metabolized via the terminal oxidation pathway of n-alkane.

Isolation and application of Gordonia sp. JC11 for removal of boat lubricants

“…The presence of various crude oildegrading microorganisms corresponded to the contamination of petroleum hydrocarbons in Thai coastal areas (Wattayakorn, 2005). Although all of the tested microorganisms were able to grow in crude oil-NSW (Aluyor and Ori-jesu, 2009;Koma et al, 2001). To screen for the effi cient lubricant-degrading strains, the isolated microorganisms were characterized for their cellular properties.…”

Plant litter and soil type drive abundance, activity and community structure of alkB harbouring microbes in different soil compartments

“…Although the chemically inert alkanes are activated by the addition of fumarate (Heider et al, 1999;Widdel and Rabus, 2001;Wilkes et al, 2003) during anoxia, aerobic activation is accomplished by the terminal (Sepic et al, 1995;Koma et al, 2001;Van Hamme et al, 2003) or subterminal (Whyte et al, 1998;Kotani et al, 2006Kotani et al, , 2007 introduction of oxygen. For alkanes with a chain length oC 30 , which can be considered as typical for plant waxes, terminal oxygen introduction is mainly catalysed by the membrane bound, rubredoxin-dependent di-iron alkane monooxygenase (AlkB), which is found among Actinobacteria, a-, b-and g-Proteobacteria (van Beilen et al, 2003;van Beilen and Funhoff, 2007).…”

Isolation and characterization of a novel paraffin wax-degrading bacterium, Pseudomonas sp strain PW-1, from petroleum-contaminated sites

“…strain M-1 has the ability to degrade a variety of n-alkanes, including very long-chain n-alkanes with carbon chain lengths of C 20 to C 44 at ambient temperature (Tani et al, 2001). In addition, Koma et al (2001) have also studied an Acinetobacter strain that can assimilate long-chain n-paraffins from the waste oil of car engines. Kotlar et al (2007) isolated and identified an Acinetobacter sp.…”