Biodegradation of kraft lignin by a newly isolated bacterial strain, Aneurinibacillus aneurinilyticus from the sludge of a pulp paper mill

Raj, Abhay; Chandra, Ram; Reddy, M. Mutha; Purohit, Hemant J.; Kapley, Atya

doi:10.1007/s11274-006-9299-x

Cited by 73 publications

(49 citation statements)

References 34 publications

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“…Several researchers reported strains of Bacillus and Paenibacillus which displayed abilities to decolourize kraft black liquor [Chandra et al, 2007[Chandra et al, , 2008Hassan and Amr, 2009]. Moreover, Raj et al [2010] reported the decolourization of black liquor by a newly isolated bacterium, Aneurinibacillus aneurinilyticus. Incredibly, after 6 days of incubation, A. aneurinilyticus was able to reduce the colour of black liquor by 58% and the lignin content by 43% [Raj et al, 2010].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, Raj et al [2010] reported the decolourization of black liquor by a newly isolated bacterium, Aneurinibacillus aneurinilyticus. Incredibly, after 6 days of incubation, A. aneurinilyticus was able to reduce the colour of black liquor by 58% and the lignin content by 43% [Raj et al, 2010]. Similarly, Chandra and Abhisshek [2011] recently reported that mixed cultures of Citrobacter sp.…”

Section: Discussionmentioning

confidence: 99%

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Newly Isolated and Characterized Bacteria with Great Application Potential for Decomposition of Lignocellulosic Biomass

Maki¹,

Idrees

Leung

et al. 2012

Microb Physiol

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This study focuses on the isolation and characterization of bacteria from municipal waste and peat to determine those bacteria with good potential for modification and decomposition of lignocellulosic biomass for industrial application. Twenty cellulase-producing bacteria belonging to four major phyla – Firmicutes, Actinobacteria, Proteobacteria and Bacteroidetes – were found when screened on carboxymethyl cellulose-containing agar. Six isolates also exhibited activities towards filter paper as the sole carbon source in salt media, while 12 exhibited activities towards xylan when screened on xylan-containing plates. Moreover, 5 isolates survived in and increased the absorbance of 1% black liquor in salt media by an average of 2.07-fold after 21 days of incubation. Similarly, these 5 isolates increased the absorbance of 0.1% pure lignin at 280 nm in salt media, indicating modification of lignin. Additionally, the Fourier transform infrared spectroscopy analysis of 1% barley straw treated for 21 days with these 5 strains showed a preference for consumption of hemicelluloses over lignin; however, a change in lignin was observed. A<i> Bacillus </i>strain (55S5) and a <i>Pseudomonas </i>strain (AS1) displayed the greatest potential for lignocellulose decomposition due to a variety of cellulase activities, as well as xylanase activity and modification of lignin. Several of these isolates have good potential for industrial use in the degradation of lignocellulosic biomass.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Newly Isolated and Characterized Bacteria with Great Application Potential for Decomposition of Lignocellulosic Biomass

Maki¹,

Idrees

Leung

et al. 2012

Microb Physiol

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“…Among them most frequently studied fungus for these applications is basidiomycete P. chrysosporium and B. adusta, whose secreted enzymes have an enormous potential for decolorizing textile dyes [35][36][37]. Similarly, recent report shows that bacteria also able to produce the lignin peroxidase, which has ability to degradation of polymeric dyes from the textile industry and paper whitening through oxidative cleavage [12,19,20,23,38]. Where as, azoreductase is the key enzyme expressed in azo dye degrading bacteria that catalyze the reductive cleavage of the azo bond, which has been identified in several Values are mean of three experiments ± S.E.M.…”

Section: Enzyme Analysismentioning

confidence: 99%

Ecofriendly biodegradation and detoxification of Reactive Red 2 textile dye by newly isolated Pseudomonas sp. SUK1

Kalyani

Telke

Dhanve

et al. 2009

Journal of Hazardous Materials

358

123

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“…The structure of natural lignin is very complex, and intact lignin is not commercially available. However, because of the similarities to natural lignin, kraft lignin (KL) has been widely used for lignin-related studies [13-17]. …”

Section: Introductionmentioning

confidence: 99%

Characterization and genomic analysis of kraft lignin biodegradation by the beta-proteobacterium Cupriavidus basilensis B-8

Shi

Chai

Tang

et al. 2013

Biotechnol Biofuels

466

328

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BackgroundLignin materials are abundant and among the most important potential sources for biofuel production. Development of an efficient lignin degradation process has considerable potential for the production of a variety of chemicals, including bioethanol. However, lignin degradation using current methods is inefficient. Given their immense environmental adaptability and biochemical versatility, bacterial could be used as a valuable tool for the rapid degradation of lignin. Kraft lignin (KL) is a polymer by-product of the pulp and paper industry resulting from alkaline sulfide treatment of lignocellulose, and it has been widely used for lignin-related studies.ResultsBeta-proteobacterium Cupriavidus basilensis B-8 isolated from erosive bamboo slips displayed substantial KL degradation capability. With initial concentrations of 0.5–6 g L-1, at least 31.3% KL could be degraded in 7 days. The maximum degradation rate was 44.4% at the initial concentration of 2 g L-1. The optimum pH and temperature for KL degradation were 7.0 and 30°C, respectively. Manganese peroxidase (MnP) and laccase (Lac) demonstrated their greatest level of activity, 1685.3 U L-1 and 815.6 U L-1, at the third and fourth days, respectively. Many small molecule intermediates were formed during the process of KL degradation, as determined using GC-MS analysis. In order to perform metabolic reconstruction of lignin degradation in this bacterium, a draft genome sequence for C. basilensis B-8 was generated. Genomic analysis focused on the catabolic potential of this bacterium against several lignin-derived compounds. These analyses together with sequence comparisons predicted the existence of three major metabolic pathways: β-ketoadipate, phenol degradation, and gentisate pathways.ConclusionThese results confirmed the capability of C. basilensis B-8 to promote KL degradation. Whole genomic sequencing and systematic analysis of the C. basilensis B-8 genome identified degradation steps and intermediates from this bacterial-mediated KL degradation method. Our findings provide a theoretical basis for research into the mechanisms of lignin degradation as well as a practical basis for biofuel production using lignin materials.

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Biodegradation of kraft lignin by a newly isolated bacterial strain, Aneurinibacillus aneurinilyticus from the sludge of a pulp paper mill

Cited by 73 publications

References 34 publications

Newly Isolated and Characterized Bacteria with Great Application Potential for Decomposition of Lignocellulosic Biomass

Newly Isolated and Characterized Bacteria with Great Application Potential for Decomposition of Lignocellulosic Biomass

Ecofriendly biodegradation and detoxification of Reactive Red 2 textile dye by newly isolated Pseudomonas sp. SUK1

Characterization and genomic analysis of kraft lignin biodegradation by the beta-proteobacterium Cupriavidus basilensis B-8

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