Biodegradation of Kerosene byAspergillus flavusUsing Statistical Experimental Designs

Abstract

The ability of different local fungal isolates to degrade kerosene in liquid medium was studied. The results showed that the percent of kerosene degradation varied among the different tested fungi and that 60-96% of kerosene was degraded after 7 days in the presence of 0.2% (v/v) of Tween 80. The absence of the surfactant led to about 28.34% decrease of biodegradation. The degradation of 2% (v/v) of kerosene by the most efficient fungus (Aspergillus flavus) was significantly influenced by the incubation period and the composition of culture medium. Statistical experimental designs were used to optimize the process of kerosene degradation by the fungus. Under optimized medium compositions and culture conditions, A. flavus degraded kerosene (100%) after 111.3 h of incubation. Optimal conditions obtained in this work provided a solid foundation for further use of A. flavus in treatment of kerosene-polluted soil. The optimized conditions were applied to bioremediate 2.5% (v/w) kerosene-polluted soil by A. flavus, and the fungus efficiently degraded kerosene after 35 days of incubation.

Bioremediation of Diesel Fuel by Fungal Consortium Using Statistical Experimental Designs

“…showed less than 96% degradation. The same isolates were used to degrade kerosene and it was found that A. fl avus had the highest degrading ability (96.31%), followed by A.niger (90.33%), while the remaining fungi were with lower activities [15]. These results confi rm that the biodegradation process depend on the type of hydrocarbon, the genus, species, and may be the strain of the fungus, as well as on nutritional and fermentation conditions.…”

“…So, A. ustus and A. alternata fi rstly degraded and assimilated the simpler fraction of alkanes and cyclic alkanes that appeared in the lower degradation % after four days, and for growth and production of hydrolyzing enzymes that enable the fungus to degrade the more complex fractions of diesel after days fi ve and six of fermentation (high degradation from 62 to 82%). It was reported that the complex aromatic fractions of hydrocarbons are more toxic to the organisms than the aliphatic fractions [15,32]. It was a) Fig.…”

“…Plackett-Burman design provides a fast and effective way to identify the important factors among a large number of variables, thereby saving time and maintaining convincing information on each parameter. RSM helps evaluate the important factors and building models to study the interactions between the variables or desirable responses [15,16].…”

Multiparameter optimization of bromate sorption on anion exchange resin by a two-step statistical strategy: Plackett–Burman and Box–Behnken experimental design

“…Response surface methodology (RSM), including central composite design and Box-Behnken design (BBD), is an efficient strategic experimental technique to determine optimal conditions for a multivariable system [17,18]. To our knowledge, P-B and RSM design, the two-step statistical experiment design has been widely employed in the optimization of various processes, such as biochemistry [19], food chemistry [20],and water treatment [21]. However, there are few reports concerning the optimization of bromate sorption on anion exchange resin.…”

Isolation and identification of potential fungal species for spent engine lubrication oil remediation in Peninsular Malaysia

“…The mean degradation percentage of spent engine oil in different species of fungi after incubation for seven days. (Ghanem, Al-Garni, & Alhomodi, 2015) Able to synthesize various types of enzymes such as lignin peroxidase, lipase, and catalase, which contributed to its capability in tolerating hydrocarbons (Gupta, 2016;Vatsyayan & Goswami, 2016) Able to produce biosurfactants (Adekunle et al, 2015;Kiran et al, 2009) Able to produce ligninolytic enzymes such as lignin peroxidase and manganese peroxidase (Ameen, Moslem, Hadi, & Al-Sabri, 2016) Six species of fungi, which produced significant reduction in spent engine oil content, were identified according to maximum query cover and highest identity percentage with lowest error value through BLAST: Penicillium simplicissimum, Aspergillus nidulans, Aspergillus niger, Trichoderma longibrachiatum, Aspergillus ustus, and Aspergillus flavus. The ability of these fungi to grow in selective media without a carbon source (BHB) confirmed their ability to utilize spent engine oil as a substrate for growth (Thenmozhi et al, 2013).…”