Biodegradation of kerosene by <i>Aspergillus ochraceus</i> NCIM‐1146

Saratale, Ganesh Dattatraya; Kalme, Satish; Bhosale, Sanjyot; Govindwar, Sanjay P.

doi:10.1002/jobm.200710337

Cited by 67 publications

(42 citation statements)

References 26 publications

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“…The major constituents of Kerosene are alkanes and cycloalkanes (65 to 70%), benzene and substituted benzene (10 to15%), naphthalene and substituted naphthalene (ASTM 2001) (Saratale et al, 2007;Wongsa et al, 2004). In this study, the greatest number of strains was isolated from the reservoir of Kerosene (13 strains); this can be related to pH that was almost neutral (pH 6.6).…”

Section: Study Of Bacterial Distribution In Various Reservoirsmentioning

confidence: 66%

“…Considering that the pH is alkaline (pH 8), it was common to isolate alkalotolerant or alkalophile strains, such as SSS9 and SSS15, related to P. alcaliphila NR024734.1 and P. stutzeri CP002622.1, respectively. Saratale et al (2007) reported that Pseudomonas strains have a mixed-functional oxidase system, inducible by nalkanes as well as polyaromatic hydrocarbons. Further more, diesel oil is a complex fuel mixture, primarily consisting of paraffinic, olefinic and aromatic hydrocarbons, and smaller quantities of substances containing sulfur, nitrogen, metals, and oxygen.…”

Section: Study Of Bacterial Distribution In Various Reservoirsmentioning

confidence: 99%

See 1 more Smart Citation

Diversity of culturable aerobic bacteria colonizing four petroleum by-products storage reservoirs

Saiuml¹,

Hamed²,

Gam³

et al. 2013

Afr. J. Microbiol. Res.

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The petroleum reservoirs are commonly exposed to bacterial contamination. The proliferation of several bacterial species, could modify the product quality, change viscosity, help other sulfatereducing, iron reducing bacteria to grow, and accelerate reservoir biocorrosion. Thus, companies of storage and transport of petroleum frequently use biocides to prohibit bacterial proliferation. However, the use of the appropriate biocide, need an obvious knowledge of bacterial population harbouring the reservoir. Thus, in this study, we intended to isolate and identify the aerobic bacterial population existing in different reservoirs of petroleum derivatives in the refinery of Bizerte, Tunisia. The isolation and identification of these microorganisms may be of great interest in bioremediation of polluted sites. Besides, it could inform about hydrocarbon specificity of these microorganisms and therefore lead to the use of suitable biocides to clean these storage reservoirs. 25 different bacterial strains were isolated from water collected from reservoirs of crude oil, gasoline, diesel oil and kerosene. The different strains were identified using morphological and biochemical characterization, as well as sequencing of 16S rRNA. The systematic study of these strains showed that they belong to six different genera. The highest strain number was affiliated to the genera of Pseudomonas and Bacillus followed by genera of Staphylococcus, Enterobacter Comamonas and Paenibacillus. The results of this study are discussed in terms of the ecological significance of these microorganisms in relation with physicochemical conditions of reservoirs and hydrocarbon composition.

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Section: Study Of Bacterial Distribution In Various Reservoirsmentioning

confidence: 66%

Section: Study Of Bacterial Distribution In Various Reservoirsmentioning

confidence: 99%

Diversity of culturable aerobic bacteria colonizing four petroleum by-products storage reservoirs

Saiuml¹,

Hamed²,

Gam³

et al. 2013

Afr. J. Microbiol. Res.

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“…Two or three distinct spots were observed on TLC plate after 48 h incubation by using intact mycelium (Table 4) indicates involvement of Aspergillus ochraceus in degradation of cholesterol, camphor and naphthalene by intact as well as immobilized mycelium. Aspergillus ochraceus also has ability to decolorize various industrial dyes and involvement in PAH degradation [28][29] . HPLC chromatogram of extracted sample after complete biodegradation confi rmed the degradation of camphor and cholesterol into several metabolites.…”

Section: Resultsmentioning

confidence: 99%

“…After the addition of 0.4 ml of hexane to the incubation medium as a substrate, the reaction mixture was incubated at 37 o C for 20 min and terminated the reaction by adding 1 ml of ice cold 20% trichloroacetic acid solution. The amount of acetaldehyde liberated was estimated using Nash reagent 16,29 .…”

Section: Introductionmentioning

confidence: 99%

Study of mixed function oxidase system in Aspergillus ochraceus (NCIM 1146)

2007

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Aspergillus ochraceus (NCIM-1146) has shown the ability to degrade cholesterol, camphor and naphthalene, when 96 h grown mycelium incubated in medium containing these organic compounds. Presence of higher level of electron transport components and biotransformation enzyme activity were observed in Aspergillus ochraceus, when grown in potato dextrose medium for 96 h. The enzyme activity preferred NADPH as a cofactor and shows inhibition in the presence of CO, indicating cytochrome P-450 mediated reactions. A signifi cant increase in the levels of electron transport components and biotransformation enzyme activity were observed in presence of different inducers (viz. cholesterol, camphor, naphthalene, veratrole, phenobarbital, n-hexane, kerosene and saffola oil) when compared with mycelium incubated in same way with similar conditions for 2 min incubation. Analyses of the products of cholesterol and camphor using HPLC and GCMS confi rm the degradation of these compounds.

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“…Because of their aggressive growth, greater biomass production and extensive hyphal growth, fungi offer potential for bioremediation technology. Fungi and other eukaryotes normally oxidize aromatic compounds using mono-oxygenase, forming a trans-diol (Saratale et al, 2007). Generally, BTHX compounds are often encountered as mixtures rather than as a single compound and the fate of these compounds is often strongly controlled by microbial activity (Mi-Seon et al, 2008).…”

mentioning

confidence: 99%

Mycoremediation of Monocyclic Aromatic Hydrocarbons by a Local Marine Aspergillus oryzae (Statistical Analysis of the Main and Substrate Interaction Effects)

2010

Egypt. J. Microbiol.

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N AROMATIC hydrocarbon utilizing fungus was isolated from a ……wastewater polluted sea spot in the Mediterranean, Alexandria, Egypt. It was identified to the species level as Aspergillus oryzae. The marine fungal isolate has the ability to remove BTHX compounds (benzene, toluene, hexylbenzene and xylene), concentrations ranged between 15 and 75 mgl -1 . Up to our knowledge, it is the first description of BTHX removal by a local marine strain of A. oryzae. The removal efficiencies of these compounds varied between 58 and 85 %, within 48hr. Experiments were conducted according to the 2 4 fraction fractorial design to identify the main and interaction effects of individual BTHX removal efficiency. A statistical interpretation of the results revealed that the main effect of benzene was significant (P < 0.05). High F values for the 4-way interaction between different hydrocarbons with low P values suggest that the interactions between individual BTHX are the most significant factors. The negative interactions between benzene and hexylbenzene, benzene and xylene concentrations were found to be statistically significant (P < 0.05). On the other hand, there was a positive significant interaction between hexylbenzene and xylene (P < 0.05). A successful trial showed very good applicability of the marine fungal isolate to remove 80.00, 95.64 and 89.00 % of benzene, toluene and xylene, respectively, in the waste effluent of Alexandria Petroleum Company after 48 hr. This effluent contains very high concentrations of BTX compounds (36.38gl -1 ). Using the diluted effluent (50%), the removal efficiency of the BTX reached distinct value of 97, 98 and 98% for benzene, toluene and xylene, respectively, after 72 hr. These significant quantitative removal capacities demonstrate the potential application of marine A. oryzae for the mycoremediation of BTX compounds from waste water systems.

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Biodegradation of kerosene by Aspergillus ochraceus NCIM‐1146

Cited by 67 publications

References 26 publications

Diversity of culturable aerobic bacteria colonizing four petroleum by-products storage reservoirs

Diversity of culturable aerobic bacteria colonizing four petroleum by-products storage reservoirs

Study of mixed function oxidase system in Aspergillus ochraceus (NCIM 1146)

Mycoremediation of Monocyclic Aromatic Hydrocarbons by a Local Marine Aspergillus oryzae (Statistical Analysis of the Main and Substrate Interaction Effects)

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