Biodegradation of high concentrations of mixed polycyclic aromatic hydrocarbons by indigenous bacteria from a river sediment: a microcosm study and bacterial community analysis

Muangchinda, Chanokporn; Yamazoe, Atsushi; Polrit, Duangporn; Thoetkiattikul, Honglada; Mhuantong, Wuttichai; Champreda, Verawat; Pinyakong, Onruthai

doi:10.1007/s11356-016-8185-9

Cited by 35 publications

(12 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The diversification also included the enrichment of taxa belonging to genera with biodegradation potentials in the more polluted city of Baoding. While some of these genera were detected as rare members of the skin microbiota in this study and in previous reports [39][40][41], their ability to degrade PAHs [21,42] warrants further attention.…”

Section: Discussionsupporting

confidence: 52%

Changes of the human skin microbiota upon chronic exposure to polycyclic aromatic hydrocarbon pollutants

Leung¹,

Tong²,

Bastien³

et al. 2020

Microbiome

View full text Add to dashboard Cite

Background: Polycyclic aromatic hydrocarbons (PAHs) are of environmental and public health concerns and contribute to adverse skin attributes such as premature skin aging and pigmentary disorder. However, little information is available on the potential roles of chronic urban PAH pollutant exposure on the cutaneous microbiota. Given the roles of the skin microbiota have on healthy and undesirable skin phenotypes and the relationships between PAHs and skin properties, we hypothesize that exposure of PAHs may be associated with changes in the cutaneous microbiota. In this study, the skin microbiota of over two hundred Chinese individuals from two cities in China with varying exposure levels of PAHs were characterized by bacterial and fungal amplicon and shotgun metagenomics sequencing. Results: Skin site and city were strong parameters in changing microbial communities and their assembly processes. Reductions of bacterial-fungal microbial network structural integrity and stability were associated with skin conditions (acne and dandruff). Multivariate analysis revealed associations between abundances of Propionibacterium and Malassezia with host properties and pollutant exposure levels. Shannon diversity increase was correlated to exposure levels of PAHs in a dose-dependent manner. Shotgun metagenomics analysis of samples (n = 32) from individuals of the lowest and highest exposure levels of PAHs further highlighted associations between the PAHs quantified and decrease in abundances of skin commensals and increase in oral bacteria. Functional analysis identified associations between levels of PAHs and abundance of microbial genes of metabolic and other pathways with potential importance in host-microbe interactions as well as degradation of aromatic compounds. Conclusions: The results in this study demonstrated the changes in composition and functional capacities of the cutaneous microbiota associated with chronic exposure levels of PAHs. Findings from this study will aid the development of strategies to harness the microbiota in protecting the skin against pollutants.

show abstract

Section: Discussionsupporting

confidence: 52%

Changes of the human skin microbiota upon chronic exposure to polycyclic aromatic hydrocarbon pollutants

Leung¹,

Tong²,

Bastien³

et al. 2020

Microbiome

View full text Add to dashboard Cite

show abstract

“…Some specific changes were observed that could be attributed to the presence of PAHs: the Actinobacteria , which were almost undetectable in the initial biofilm sample, increased up to 18% in the HMN and 2MN cultures and to almost 30% of the community in the NAP enrichment. Although an increase in the abundance of Actinobacteria has been related to the degradation of PAHs in aerobic conditions (Muangchinda et al ., ; Zhu et al ., ), there is no evidence of the contribution of this phylum to anaerobic degradation. Interestingly, an increase in the Acidobacteria candidate class iii1‐8 (order DS‐18) from undetectable levels in the initial biofilm sample to more than 9% of the community in the 2MN enrichment was also observed (Fig.…”

Section: Resultsmentioning

confidence: 99%

Naphthalene biodegradation under oxygen‐limiting conditions: community dynamics and the relevance of biofilm‐forming capacity

Abercron

Marín

Solsona-Ferraz

et al. 2017

Microbial Biotechnology

View full text Add to dashboard Cite

SummaryToxic polycyclic aromatic hydrocarbons (PAHs) are frequently released into the environment from anthropogenic sources. PAH remediation strategies focus on biological processes mediated by bacteria. The availability of oxygen in polluted environments is often limited or absent, and only bacteria able to thrive in these conditions can be considered for bioremediation strategies. To identify bacterial strains able to degrade PAHs under oxygen‐limiting conditions, we set up enrichment cultures from samples of an oil‐polluted aquifer, using either anoxic or microaerophilic condition and with PAHs as the sole carbon source. Despite the presence of a significant community of nitrate‐reducing bacteria, the initial community, which was dominated by Betaproteobacteria, was incapable of PAH degradation under strict anoxic conditions, although a clear shift in the structure of the community towards an increase in the Alphaproteobacteria (Sphingomonadaceae), Actinobacteria and an uncultured group of Acidobacteria was observed in the enrichments. In contrast, growth under microaerophilic conditions with naphthalene as the carbon source evidenced the development of a biofilm structure around the naphthalene crystal. The enrichment process selected two co‐dominant groups which finally reached 97% of the bacterial communities: Variovorax spp. (54%, Betaproteobacteria) and Starkeya spp. (43%, Xanthobacteraceae). The two dominant populations were able to grow with naphthalene, although only Starkeya was able to reproduce the biofilm structure around the naphthalene crystal. The pathway for naphthalene degradation was identified, which included as essential steps dioxygenases with high affinity for oxygen, showing 99% identity with Xanthobacter polyaromaticivorans dbd cluster for PAH degradation. Our results suggest that the biofilm formation capacity of Starkeya provided a structure to allocate its cells at an appropriate distance from the toxic carbon source.

show abstract

“…Co‐metabolic pathways and cross‐feeding among microorganisms are common phenomena in activated sludge. It has been reported that numerous bacterial genera and species are responsible for PAH degradation . In this study, the presence of some bacterial species bioaugmented our activated sludge.…”

Section: Resultsmentioning

confidence: 82%

“…Aromatic hydrocarbons are described as teratogenic, mutagenic, carcinogenic and potent immune suppressants due to their toxicity . Priority has been given to remove 16 PAHs from environmental systems by the US Environmental Protection Agency (EPA) …”

Section: Introductionmentioning

confidence: 99%

Monitoring of the degradation of aromatic hydrocarbons by bioaugmented activated sludge

Vural

Özdemir

2019

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

BACKGROUND: The petrochemical industry wastewater contains many organic compounds that are difficult to degrade. The activated sludge system reinforced by bioaugmentation technology represents a more effective alternative to treat industrial wastewater. In this study, bioaugmented activated sludge water demonstrated high efficiency in removing five tested hydrocarbons.RESULTS: Optimized degradation rates were obtained upon inoculation of a continuous bioreactor system with degradative bacteria (Acinetobacter sp., Aeromonas sp., Arthrobacter nicotianae, Burkholderia cepacia, Chryseobacterium sp., Pseudomonas fluorescens, Micrococcus luteus and Raoultella planticola). The addition of a hydrocarbon mixture (terephthalic acid, acenaphthene, fluorene, anthracene, and pyrene) into the bioaugmented sludge resulted in degradation rates equivalent to 97.90%, 99.87%, 98.89%, 97.28%, and 94.55% respectively after 24-48 h incubation. Molecular biomonitoring was performed with real-time quantitative polymerase chain reaction (qPCR). According to the obtained results, the numbers of copy DNA of the degradative bacteria increased by 0.36-1.13 log at the end of the degradation process. The presence of some oxygenase genes (Nah, NidA, Pah Rhd Gr(+), Pah Rhd Gr(−) and C23O), that catabolize aromatic hydrocarbons in activated sludge, were detected by specific primers. CONCLUSION: Bioaugmentation represents a potent and eco-friendly approach that can effectively remove the drastic increase in the concentrations of aromatic hydrocarbons in industrial wastewaters. Molecular biomonitoring during degradation can be considered a rapid and an effective in situ method that quantifies the active microbial population in wastewater. The method provides beneficial information about the composition of microbial community and sustainability of the degradation process. Figure 3. The graphics reveal the alterations in the copy number of DNA of bacterial isolates in bioaugmented sludge during the three stages (n = 3, ± standard deviation). (a-c) The numbers of bacteria at the first, second and third stages and the copy number of DNA in the first, second and third stages, respectively. The copy number of DNA of all bacterial isolates have increased as the concentration of hydrocarbon increased. J Chem Technol Biotechnol 2020; 95: 52-62

show abstract

Biodegradation of high concentrations of mixed polycyclic aromatic hydrocarbons by indigenous bacteria from a river sediment: a microcosm study and bacterial community analysis

Cited by 35 publications

References 56 publications

Changes of the human skin microbiota upon chronic exposure to polycyclic aromatic hydrocarbon pollutants

Changes of the human skin microbiota upon chronic exposure to polycyclic aromatic hydrocarbon pollutants

Naphthalene biodegradation under oxygen‐limiting conditions: community dynamics and the relevance of biofilm‐forming capacity

Monitoring of the degradation of aromatic hydrocarbons by bioaugmented activated sludge

Contact Info

Product

Resources

About