Biodegradation of Fluoranthene by Basidiomycetes Fungal Isolate Pleurotus Ostreatus HP-1

Abstract

The biodegradation of fluoranthene, a high molecular weight polycyclic aromatic hydrocarbon (PAH), was investigated in submerged culture using the wood decaying fungus isolated from forest locality in Gujarat, India. The basidiomycete fungal isolate was found to have an ability to grow on sabaroud dextrose agar containing 50 mgl(-1) of each naphthalene, anthracene, acenaphthene, benzo (a) anthracene, pyrene, flouranthene, carbazole, and biphenyl. The involvement of extracellular fungal peroxidases such as manganese peroxidase (MnP) and laccase (Phenol oxidase) in the degradation of fluoranthene was studied. On the eighth day of incubation 54.09% of 70 mg l(-1) fluoranthene was removed. There after no PAHs removal was observed till the 20th day of the incubation period. The isolate was identified as Pleurotus ostreatus by 18S rRNA, 5.8S rRNA, and partial 28S rRNA gene sequencing. To the best of our knowledge this is the first time Pleurotus ostreatus have been reported to degrade such a high concentration of fluoranthene within much lower time period of incubation. Depletion in the residual fluoranthene in the culture medium was determined by HPLC. Attempts were made to identify the degradation product in the culture medium with the help of FT-IR, NMR, and HPTLC analysis. In the present study positive correlation between fluoranthene degradation and the ligninolytic enzyme (MnP and laccase) production is observed, thus this isolate can play an effective role for bioremediation of PAHs contaminated sites.

Biodegradation of oxytetracycline by Pleurotus ostreatus mycelium: a mycoremediation technique

“…Fungi have not extensively investigated for biotransformation of antibiotics [12]. Some of them have been applied in polycyclic aromatic compounds bioremediation and dyes decolorization [13][14][15]. Wen et al [16,17] studied the degradation of tetracycline and OTC by crude ligninolytic enzymes (lignin peroxidase and manganese peroxidase) prepared from Phanerochaete chrysosporium, with positive results.…”

Optimization of culture condition for enhanced decolorization and degradation of azo dye reactive violet 1 with concomitant production of ligninolytic enzymes by Ganoderma cupreum AG-1

“…In the recent past there has been an intensive research on bioremediation of dyes, and the use of white rot fungi/ligninolytic fungi is turning into a promising alternative to replace or supplement present treatment processes (Dos-Santos et al 2004; Asgher et al 2006). The abilities of white rot fungi in mineralization of xenobiotics to CO 2 and water through the highly oxidative and nonspecific ligninolytic system are well documented, which are also responsible for decolorization and degradation of wide range of dyes (Boer et al 2004; Patel et al 2009) The extracellular enzyme system includes Laccase (E.C. 1.10.3.2), Manganese peroxidase (MnP, E.C.…”

Fates of nickel and fluoranthene during the bioremediation by Pleurotus eryngii in three different soils

“…The objectives of this study were to investigate: (1) the effects of Ni and fluoranthene on the growth of P. eryngii in sandy loam, loamy clay, and sandy soils; (2) the combined effects of co-contaminants on the accumulation of Ni and the degradation of fluoranthene by P. eryngii in three types of contaminated soils;…”