Biodegradation of diisononyl phthalate by a consortium of saline soil bacteria: optimisation and kinetic characterisation

Pereyra-Camacho, Marco A.; Balderas‐Hernández, Víctor E.; León‐Rodríguez, Antonio De

doi:10.1007/s00253-021-11255-5

Cited by 24 publications

(9 citation statements)

References 40 publications

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“…Pereyra-Camacho et al observed a consortia of saline soil bacteria with Achromobactor spp., Pseudomonas spp., and Brevundimonas spp. (Pereyra-Camacho et al, 2021 ) degrading diisononyl phthalate, a common plasticizer; these three genera were identified in our study . Achromobactor denitrificans PR1 is a known sulfonamide degrader that has been isolated from wastewater activated sludge, although this ability does not seem widespread in other Achromobactor spp.…”

Section: Discussionsupporting

confidence: 62%

Versatile microbial communities rapidly assimilate ammonium hydroxide-treated plastic waste

Schaerer

Wood

Aloba

et al. 2023

Journal of Industrial Microbiology and Biotechnology

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Most plastic waste accumulates in landfills or the environment. Natural microbial metabolisms can degrade plastic polymers. Unfortunately, biodegradation of plastics is slow even under ideal conditions; depolymerization of plastic is the rate limiting step. Rapid chemical depolymerization yields biodegradable plastic monomers, improving biodegradation rates. Here we demonstrate that ammonium hydroxide depolymerizes PET into terephthalic acid and terephthalic acid monoamide which are rapidly metabolized by diverse consortia obtained from compost and sediment. By neutralizing the product with phosphoric acid prior to bioprocessing, the final product contains plastic-derived carbon and biologically accessible nitrogen and phosphorus from the process reactants, removing the need for culture medium. Three microbial consortia were able to degrade chemically deconstructed PET in ultrapure water and scavenged river water without the addition of nutrients, with no statistically significant difference in growth rate compared to communities grown on deconstructed PET in Bushnell Haas minimal culture medium. The consortia were dominated by Rhodococcus spp., Hydrogenophaga spp., and many lower abundance genera. All taxa were related to species known to degrade aromatic compounds. Microbial consortia are known to confer flexibility in processing diverse substrates. To highlight the versatility of these consortia, we also demonstrate that two microbial consortia can grow on similarly deconstructed polyesters, polyamides, and polyurethanes in water instead of medium. Our findings suggest that using microbial communities enable flexible bioprocessing of mixed plastic wastes. We also demonstrate the flexibility of this approach for coupled chemical deconstruction and bioprocessing.

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Section: Discussionsupporting

confidence: 62%

Versatile microbial communities rapidly assimilate ammonium hydroxide-treated plastic waste

Schaerer

Wood

Aloba

et al. 2023

Journal of Industrial Microbiology and Biotechnology

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“…Di‐(2‐ethylhexyl) phthalate, a widely used phthalate ester, is recalcitrant due to its long side chain. Many microorganisms are known to mineralize phthalate esters through pathways that involve an aromatic carboxylesterase (Ding et al ., 2015; Mahajan et al ., 2019; Wang et al ., 2019; Huang et al ., 2020; Pereyra‐Camacho et al ., 2021). Alcanivorax sp.…”

Section: Enzymology Of Phthalate Estersmentioning

confidence: 99%

Environmental role of aromatic carboxylesterases

Ghodke

Punekar

2021

Environmental Microbiology

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Summary The carboxylesterases (EC 3.1.1.x) are widely distributed and form an important yet diverse group of hydrolases catalysing the ester bond cleavage in a variety of substrates. Besides acting on plant cell wall components like cutin, tannin and feruloyl esters, they are often the first line of defence to metabolize drugs, xenobiotics, pesticides, insecticides and plastic. But for the promiscuity of some carboxylesterases and cutinases, very few enzymes act exclusively on aromatic carboxylic acid esters. Infrequent occurrence of aromatic carboxylesterases suggests that aromatic carboxylesters are inherently more difficult to hydrolyse than the regular carboxylesters because of both steric and polar effects. Naturally occurring aromatic carboxylesters were rare before the anthropogenic activity augmented their environmental presence and diversity. An appraisal of the literature shows that the hydrolysis of aromatic carboxylic esters is a uniquely difficult endeavour and hence deserves special attention. Enzymes to hydrolyse such esters are evolving rapidly in nature. Very few such enzymes are known and they often display much lower catalytic efficiencies. Obviously, the esters of aromatic carboxylic acids, including polyethylene terephthalate waste, pose an environmental challenge. In this review, we highlight the uniqueness of aromatic carboxylesters and then underscore the importance of relevant carboxylesterases.

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“…In our previous study, we showed that DINCH could cause hatching delay, result in a slight lipid accumulation, and alter the expression of genes involved in lipid transport, cholesterol biosynthesis, and homeostasis in zebrafish 47 . In the present study, DINP was selected since it has been listed in water and environmental pollutants by European Protection Agency 48 . DINCH was involved in this study, because it is produced by catalytic hydrogenation of the aromatic ring of DINP and it becomes one of the most dominant used alternative plasticizer worldwide 49 .…”

Section: Introductionmentioning

confidence: 99%

“…47 In the present study, DINP was selected since it has been listed in water and environmental pollutants by European Protection Agency. 48 DINCH was involved in this study, because it is produced by catalytic hydrogenation of the aromatic ring of DINP and it becomes one of the most dominant used alternative plasticizer worldwide. 49 The controversial or no negative effect results on DINCH are primarily from the industry that produces it, 37 and thus, there is a need to better demonstrate and understand the effects of DINCH at the molecular level.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of diisononyl phthalate and di(isononyl)cyclohexane‐1,2 dicarboxylate plasticizers in regulation of lipid metabolism in 3T3‐L1 cells

Bereketoglu,

Häggblom,

Turanlı

et al. 2023

Environmental Toxicology

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Diisononyl phthalate (DINP) and di(isononyl)cyclohexane‐1,2‐dicarboxylate (DINCH) are plasticizers introduced to replace previously used phthalate plasticizers in polymeric products. Exposure to DINP and DINCH has been shown to impact lipid metabolism. However, there are limited studies that address the mechanisms of toxicity of these two plasticizers. Here, a comparative toxicity analysis has been performed to evaluate the impacts of DINP and DINCH on 3T3‐L1 cells. The preadipocyte 3T3‐L1 cells were exposed to 1, 10, and 100 μM of DINP or DINCH for 10 days and assessed for lipid accumulation, gene expression, and protein analysis. Lipid staining showed that higher concentrations of DINP and DINCH can induce adipogenesis. The gene expression analysis demonstrated that both DINP and DINCH could alter the expression of lipid‐related genes involved in adipogenesis. DINP and DINCH upregulated Pparγ, Pparα, C/EBPα Fabp4, and Fabp5, while both compounds significantly downregulated Fasn and Gata2. Protein analysis showed that both DINP and DINCH repressed the expression of FASN. Additionally, we analyzed an independent transcriptome dataset encompassing temporal data on lipid differentiation within 3T3‐L1 cells. Subsequently, we derived a gene set that accurately portrays significant pathways involved in lipid differentiation, which we subsequently subjected to experimental validation through quantitative polymerase chain reaction. In addition, we extended our analysis to encompass a thorough assessment of the expression profiles of this identical gene set across 40 discrete transcriptome datasets that have linked to diverse pathological conditions to foreseen any potential association with DINP and DINCH exposure. Comparative analysis indicated that DINP could be more effective in regulating lipid metabolism.

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Biodegradation of diisononyl phthalate by a consortium of saline soil bacteria: optimisation and kinetic characterisation

Cited by 24 publications

References 40 publications

Versatile microbial communities rapidly assimilate ammonium hydroxide-treated plastic waste

Versatile microbial communities rapidly assimilate ammonium hydroxide-treated plastic waste

Environmental role of aromatic carboxylesterases

Comparative analysis of diisononyl phthalate and di(isononyl)cyclohexane‐1,2 dicarboxylate plasticizers in regulation of lipid metabolism in 3T3‐L1 cells

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