Biodegradation of diesel oil and production of fatty acid esters by a newly isolated Pseudomonas citronellolis KHA

Abstract

The ability of a newly isolated Pseudomonas citronellolis KHA to degrade diesel oil and to synthesize fatty acid esters has been screened in aerobic batch cultures. The microorganism was able to grow with diesel oil at initial concentrations up to 126 g/l, with optimal growth at 25 g/l. Strain KHA has produced compounds showing strong emulsifying properties (E 24 = 75% at the end of the exponential growth phase). The crude extract reduces the surface tension of water from 72 mN m -1 down to 35 mN m -1 with a corresponding minimal concentration value of 60 mg/l. GC and GC-MS analysis of crude product show that the major components are those of hexadecanoic acid propyl ester and octadecanoic acid propyl ester, which have potential for applications in cosmetics, pharmaceutical and foods industries. In addition, strain KHA represents a valuable source of compounds with surface-active properties and potential for the application in clean up of the sites contaminated with hydrocarbons.

High molecular weight bioemulsifiers, main properties and potential environmental and biomedical applications

“…A complex of lipids, proteins and polysaccharids bioemulsifier produced by Candida lipolytica formed stable emulsions with hydrophobic natural compounds (Rufino et al 2007). In addition, fatty acid esters produced by P. citronellolis KHA showed greater emulsifying properties with an E 24 of 75 % at the end of the exponential growth phase permitting its use in the cleanup of the sites contaminated with hydrocarbons (Sadouk et al 2009). Besides that, whole bacterial cells of Pseudomonas marginalis PD-14B (Burd and ward 1996) and Micrococcus luteus BN56 (Tuleva et al 2009) were reported as interesting emulsifying agents.…”

“…They can be produced from inexpensive raw materials that are available in large quantities, such as industrial wastes (Panilaitis et al 2007) and oily byproducts including hydrocarbons (Kaeppeli and Finnery 1979;Leahy et al 2003;MartinezCheca et al 2007;Arli et al 2011) and vegetable oils (Liu et al 2011). In many cases, they are produced by hydrocarbon degrading bacteria during their growth (Kaeppeli and Finnery 1979;MacDonald et al 1981;Pines and Gutnick 1986;Peng et al 2007;Sadouk et al 2009). Furthermore, the production efficiency of the bioemulsifiers using microorganisms has been improved along with the progress of biotechnology by the amelioration of fermentation conditions (Doshi et al 2010) and the optimization of production by means of the response surface methodology (Prapulla et al 1992;Albuquerque et al 2006;Su et al 2009).…”

Enhanced phenol degradation by immobilized Acinetobacter sp. strain AQ5NOL 1

“…Degradation of pollutants and toxic compounds in the environment are mainly due microbial activities as shown in numerous reports such as the bioremediation of pesticides (Song et al 2005;Chatterjee et al 2010), diesel (Sadouk et al 2009;Moslemy et al 2002), azo dyes (Syed et al 2009;Revankar and Lele 2006;González-Gutiérrez et al 2009), heavy metals (Shukor et al 2010;Zhou et al 2007;Ozdemir et al 2005) and phenol derivatives (Sejákova et al 2009;Aguayo et al 2009;Machado et al 2005;Č ejková et al 2005). However, the growth of these microorganisms is inhibited at high concentrations of the xenobiotics, thus limiting the efficiency of the biodegradation of phenols (Prieto et al 2002).…”

Characterization of 2T engine oil degrading indigenous bacteria, isolated from high altitude (Mussoorie), India

“…A large number of bacteria for example Pseudomonas citronellolis KHA (Sadouk et al 2009), Yokenella spp., Stenotrophomonas spp., Alcaligens spp., Roseomonas spp., Alcaligens spp., Roseomonas spp., Flavobacter spp., Corynebacterium spp., Streptococcus spp., Providencia spp., Sphingobacterium spp., Capnocytophaga spp., Moraxella spp., Bacillus spp. (Rusansky et al 1987;Antai 1990;Bhattacharya et al 2002;Herrera et al 2008), Enterobacter spp., Escherichia spp.…”