Biodegradation of di‑n‑butyl phthalate by psychrotolerant Sphingobium yanoikuyae strain P4 and protein structural analysis of carboxylesterase involved in the pathway

Mahajan, Rishi; Verma, Shalini; Kushwaha, Madhulika; Singh, Dharam; Akhter, Yusuf; Chatterjee, Subhankar

doi:10.1016/j.ijbiomac.2018.10.225

Cited by 46 publications

(18 citation statements)

References 47 publications

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“…Microbial degradation especially bacteria-mediated biodegradation is considered the most promising method for removing PAEs from polluted environments. Several microorganisms and their related critical enzymes capable of degrading PAEs were summarized in Table 1, bacteria from genus of Sphingobium [8], Pseudomonas [9], Bacillus [10], Sulfobacillus [11], Acinetobacter [12], Rhodococcus [13], Fusarium [14,15], Gordonia [16], and Micrococcus [17] were included. Moreover, esterases from tissues [18] or uncultured microorganisms [19] with PAEs biodegradation ability were also reported.…”

Section: Introductionmentioning

confidence: 99%

Development of a whole-cell biocatalyst for diisobutyl phthalate degradation by functional display of a carboxylesterase on the surface of Escherichia coli

et al. 2020

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Background: Phthalic acid esters (PAEs) are widely used as plasticizers or additives during the industrial manufacturing of plastic products. PAEs have been detected in both aquatic and terrestrial environments due to their overuse. Exposure of PAEs results in human health concerns and environmental pollution. Diisobutyl phthalate is one of the main plasticizers in PAEs. Cell surface display of recombinant proteins has become a powerful tool for biotechnology applications. In this current study, a carboxylesterase was displayed on the surface of Escherichia coli cells, for use as whole-cell biocatalyst in diisobutyl phthalate biodegradation. Results: A carboxylesterase-encoding gene (carEW) identified from Bacillus sp. K91, was fused to the N-terminal of ice nucleation protein (inpn) anchor from Pseudomonas syringae and gfp gene, and the fused protein was then cloned into pET-28a(+) vector and was expressed in Escherichia coli BL21(DE3) cells. The surface localization of INPN-CarEW/ or INPN-CarEW-GFP fusion protein was confirmed by SDS-PAGE, western blot, proteinase accessibility assay, and green fluorescence measurement. The catalytic activity of the constructed E. coli surface-displayed cells was determined. The cell-surface-displayed CarEW displayed optimal temperature of 45 °C and optimal pH of 9.0, using p-NPC 2 as substrate. In addition, the whole cell biocatalyst retained ~ 100% and ~ 200% of its original activity per OD 600 over a period of 23 days at 45 °C and one month at 4 °C, exhibiting the better stability than free CarEW. Furthermore, approximately 1.5 mg/ml of DiBP was degraded by 10 U of surface-displayed CarEW cells in 120 min. Conclusions: This work provides a promising strategy of cost-efficient biodegradation of diisobutyl phthalate for environmental bioremediation by displaying CarEW on the surface of E. coli cells. This approach might also provide a reference in treatment of other different kinds of environmental pollutants by displaying the enzyme of interest on the cell surface of a harmless microorganism.

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Section: Introductionmentioning

confidence: 99%

Development of a whole-cell biocatalyst for diisobutyl phthalate degradation by functional display of a carboxylesterase on the surface of Escherichia coli

et al. 2020

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“…Furthermore, a conserved His-Gly-Gly-Gly tetrapeptide sequence is identified in strain P4, which is a motif included in the α/β hydrolase family. The sequence has been previously reported in mono-alkyl and di-alkyl phthalate ester hydrolysis (Mahajan et al, 2019). The positively charged residue in the motif may play an essential role in neutralizing the carboxylate anions to prevent repulsion between the catalytic center and the anion near the ester bond of the phthalate (Mahajan et al, 2019).…”

Section: Dbp Degradation By Sphingobium Spmentioning

confidence: 65%

“…Two strains of the Sphingobium genus degrade DBP with high efficiency. Sphingobium yanoikuyae strain P4, a Gramnegative bacterium, was isolated from a waste dumping site in the West Himalayas of India (Mahajan et al, 2019). It is able to degrade a high concentration of DBP of 1,000 mg/L in 24 hours at 28 • C. Such high concentration is far above the solubility as pointed out above ( (Gu, 2021).…”

Section: Dbp Degradation By Sphingobium Spmentioning

confidence: 97%

Degradability and Biochemical Pathways of the Endocrine-disrupting Plasticizers Phthalate Esters in Plastics by Microorganisms

Mak

2021

Appl Env Biotechnol

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Phthalate esters (PAEs) are a group of endocrine-disrupting organic chemicals commonly used as additives in the manufacturing of a wide range of plastics. Large quantities of different phthalate esters have been used in specific products for quality and performance by the manufacturing industries, and they pose a significant risk to human health and the ecological quality of the environments due to leaching out of phthalates from plastic products and their high mobility. Since phthalate esters are most removed efficiently through biodegradation by microorganisms in the environments, it is important to understand the efficiency, microorganisms involved, biochemical transformation processes and mechanisms of phthalate metabolism by the specific microorganisms. This article addresses the degradation of endocrine-disrupting phthalates and their fates by an integrative comparison and analysis on efficient PAEs-degrading microorganisms, the microbial metabolism, and the biochemical processes and limitation. The comparison reveals that no significant difference is evident on efficiencies between single strains of bacteria or the mixed bacterial consortia when degradation can be carried out. However, there are a few important characteristics among the efficiencies of the PAEs-degrading bacteria. The microorganisms shall utilize the specific phthalate ester as the sole source of carbon and energy. They shall mineralize the substrates, including the original compound and its degradation intermediates to achieve a complete removal. In addition, it is of practical importance for the bacteria to adapt and survive in a range of temperatures, salinity and pH as well as in the presence of indigenous microorganisms in bioremediation of contaminated sites or wastewater treatment. This review also reveals that caution should be given to both the presentation and interpretation of the degradation results for a comprehensive knowledge, particularly data on bacterial growth, extraction and analysis of residual PAEs, and the confounding use of surfactants or co-substrate in the research. The public awareness of plasticizers as an environmental pollutant is mostly due to its increasing quantities being used, constant contacts with human population on a daily basis and potential health hazards. Its toxicity shall be address more focused on reproductive biology meaningfully than the traditional mortality test in toxicology for the significant effects on animals including human.

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“…In Achromrobacter denitrificans SPI, the complete consumption of 2780 mg L -1 (10 mM) within 24 h appears to be the highest rate reported so far, however, no quantitative correlation between biomass production and DBP consumption was presented (Benjamin et al 2016). The degradation of 1000 mg DBP L -1 in 12 h in S. yanoikuyae strain P4 was accompanied by growth to OD 600 = 1.4 (Mahajan et al 2019), which is in the range of the values obtained with Paenarthrobacter strain Shss. In Rhodococcus sp.…”

Section: Analysis Of Culture Intermediates During Dbp Degradation By Paenarthrobacter Strain Shssmentioning

confidence: 86%

Degradation of dibutyl phthalate by Paenarthrobacter sp. Shss isolated from Saravan landfill, Hyrcanian Forests, Iran

et al. 2021

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Phthalic acid esters are predominantly used as plasticizers and are industrially produced on the million ton scale per year. They exhibit endocrine-disrupting, carcinogenic, teratogenic, and mutagenic effects on wildlife and humans. For this reason, biodegradation, the major process of phthalic acid ester elimination from the environment, is of global importance. Here, we studied bacterial phthalic acid ester degradation at Saravan landfill in Hyrcanian Forests, Iran, an active disposal site with 800 tons of solid waste input per day. A di-n-butyl phthalate degrading enrichment culture was established from which Paenarthrobacter sp. strain Shss was isolated. This strain efficiently degraded 1 g L–1 di-n-butyl phthalate within 15 h with a doubling time of 5 h. In addition, dimethyl phthalate, diethyl phthalate, mono butyl phthalate, and phthalic acid where degraded to CO2, whereas diethyl hexyl phthalate did not serve as a substrate. During the biodegradation of di-n-butyl phthalate, mono-n-butyl phthalate was identified in culture supernatants by ultra-performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry. In vitro assays identified two cellular esterase activities that converted di-n-butyl phthalate to mono-n-butyl phthalate, and the latter to phthalic acid, respectively. Our findings identified Paenarthrobacter sp. Shss amongst the most efficient phthalic acid esters degrading bacteria known, that possibly plays an important role in di-n-butyl phthalate elimination at a highly phthalic acid esters contaminated landfill.

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Biodegradation of di‑n‑butyl phthalate by psychrotolerant Sphingobium yanoikuyae strain P4 and protein structural analysis of carboxylesterase involved in the pathway

Cited by 46 publications

References 47 publications

Development of a whole-cell biocatalyst for diisobutyl phthalate degradation by functional display of a carboxylesterase on the surface of Escherichia coli

Development of a whole-cell biocatalyst for diisobutyl phthalate degradation by functional display of a carboxylesterase on the surface of Escherichia coli

Degradability and Biochemical Pathways of the Endocrine-disrupting Plasticizers Phthalate Esters in Plastics by Microorganisms

Degradation of dibutyl phthalate by Paenarthrobacter sp. Shss isolated from Saravan landfill, Hyrcanian Forests, Iran

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