Biodegradation of crude oil by a defined co-culture of indigenous bacterial consortium and exogenous Bacillus subtilis

Tao, Kaiyun; Liu, Xiaoyan; Chen, Xueping; Hu, Xiaoxin; Cao, Liya; Yuan, Xiaoyu

doi:10.1016/j.biortech.2016.10.073

Cited by 119 publications

(51 citation statements)

References 33 publications

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“…With the addition of 40 µg/ml biosurfactant (rhamnolipid JBR-425), another 10% improvement was observed. In another study by Tao et al (2017), the indigenous bacterial consortium achieved a degradation ratio of 71.32%. Addition of Bacillus subtilis strain ZF3-1 to the consortium enhanced the degradation efficiency to 85.01%.…”

Section: Crude Oil Depletion By the Constructed Consortiummentioning

confidence: 93%

Enhanced crude oil depletion by constructed bacterial consortium comprising bioemulsifier producer and petroleum hydrocarbon degraders

Xia¹,

Chakraborty

et al. 2019

Bioresource Technology

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The aim of this work was to study the production of bioemulsifier by Rhodococcus erythropolis OSDS1, and the improvement of crude oil depletion efficiency using a consortium of petroleum hydrocarbon degraders and OSDS1. The results showed that R. erythropolis OSDS1 produced highly stable bioemulsifier under various salinity (0-35 g/L NaCl) and pH (5.0-9.0) conditions; more than 90% of the initial emulsification activity was retained after 168 h. Emulsification capacity of the bioemulsifier on different petroleum hydrocarbons was diesel > mineral oil/crude oil > gasoline. A mixed bacterial consortium combining OSDS1 and four other petroleum hydrocarbon degraders was constructed. GC-MS results revealed that the constructed consortium achieved 85.26% depletion efficiency of crude oil in 15 days, which was significantly higher than that of individual strains. During the process, alkane hydroxylase gene (alkB) was successfully amplified from the consortium, confirming presence of crude oil degrading enzymes.

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Section: Crude Oil Depletion By the Constructed Consortiummentioning

confidence: 93%

Enhanced crude oil depletion by constructed bacterial consortium comprising bioemulsifier producer and petroleum hydrocarbon degraders

Xia¹,

Chakraborty

et al. 2019

Bioresource Technology

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“…However, the heatmap of the selected genera (relative abundance > 0.01) showed the differences among the SOIL, CW, and bioremediation samples ( Figure 4B). As expected, the relative abundance of some genera which may play important roles in crude oil biodegradation increased in the bioaugmentation treatment groups, such as Arthrobacter, Pseudomonas, Bacillus, Alcaligenes, Acinetobacter, and so on (Ollivier and Magot, 2005;Banerjee and Ghoshal, 2010;Pandey et al, 2016;Tao et al, 2017). There were only 152 core OTUs in all samples, while the SOIL sample possessed the largest number of specific OTUs at 436, which is far more than the other five samples (CW at 22, BAI at 12, BAO at 4, BAM at 6, BAB at 7) ( Figure 4C).…”

Section: Shifts In Soil Microbial Communitymentioning

confidence: 53%

Petroleum Depletion Property and Microbial Community Shift After Bioremediation Using Bacillus halotolerans T-04 and Bacillus cereus 1-1

et al. 2020

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Bioremediation of crude oil contaminated environments is an economical, lowmaintenance, environmentally friendly technology and has attracted increasing attention in recent years. In the present study, two efficient crude oil degrading bacteria strains were isolated from soils contaminated with crude oil. Phylogenetic analysis suggested they belonged to genus Bacillus, and were designated as Bacillus cereus T-04 and Bacillus halotolerans 1-1. The crude oil depletion of each strain under different conditions was tested. The optimum conditions for both strains' oil degradation was pH 7, 15-20 g/L NaCl concentration, and 5-15 g/L original oil concentration. The crude oil depletion rate could reach to 60-80% after 20 days of treatment. The crude oil bioremediation simulation tests revealed that the bioremediation promoted the depletion of crude oil to a large extent. The inoculum group with inorganic medium showed the highest crude oil depletion (97.5%) while the crude oil depletion of control group was only 26.6% after 180 days of treatment. High-throughput sequencing was used to monitor the changes of microbial community using different treatments. In all groups, Actinobacteria, Proteobacteria, Firmicutes and Bacteroidetes were the dominant phyla. After contaminated with crude oil, the relative abundance of phylum Actinobacteria was dramatically increased and occupied 81.8%. Meanwhile although strains of Bacillus were added in the bioaugmentation groups, the relative abundance of genus Bacillus was not the most abundant genus at the end of simulation tests. The crude oil contamination dramatically decreased the soil microbial diversity and bioremediation could not recover the microbial community in the short term.

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“…Microorganism DNA in cucumber rhizospheric soil was extracted by using an EZNA® Soil DNA Kit (Omega Bio-tek, Norcross, GA, USA). Then, 16S rRNA amplicon sequencing and data processing were performed at Personal Biotechnology Co., Ltd. (Shanghai, China) [41].…”

Section: S Rrna Amplicon Sequencing Of Microorganisms In Rhizosphermentioning

confidence: 99%

Metagenomic analysis of cucumber rhizospheric microbiome provides insights into NaHCO3 stress mitigation by Rheinheimera pacifica NYJ

et al. 2020

Preprint

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Background: NaHCO3 in soil leads to alkalinization and causes stress in plants, thus hindering agricultural sustainability. A strain NYJ was identified as Rheinheimera pacifica and applied into cucumber-planted soil to mitigate NaHCO3 stress in this study. We aimed to detect the taxonomy and functional potential of rhizospheric microbiome affected by NaHCO3 stress and NYJ application, obtaining NaHCO3-tolerant microbes and exploring one mechanism underlining NaHCO3 stress mitigation by NYJ application.Results: The strain NYJ grew well under 0.01-0.06 g L-1 NaHCO3 and had plant growth-promoting capacity. Application of NYJ into NaHCO3-contaminated and cucumber-planted soil improved plant growth, increased relative water contents and osmotic potential of leaves, and decreased malonaldehyde and hydrogen peroxide contents in seedlings, mitigating NaHCO3 stress in cucumber. In cucumber rhizospheric soil, Proteobacteria, Actinobacteria, and Bacteroidetes of microbes were dominant phyla with 69, 17, and 6% of total abundance. To respond to NaHCO3 stress, 669 species and 208 genera of microbes were enriched in rhizospheric soil, while 190 sodium ion-related genes, 1242 osmotic stress-related genes, and 121 other stress-related genes from microbial genera including Pseudomonas showed the increased abundances. Under NaHCO3 stress, 26 microbial genera were distributed in connectors, the interaction network of rhizospheric microbes was changed, and soil enzyme activities were decreased. When NYJ being applied into the NaHCO3-contaminated soil, 1327 species and 487 genera of microbes were enriched than those under NaHCO3 stress alone. Meantime, soil enzyme activities were increased, while 2238 symbiont- and immune-related genes and 2434 plant growth-promoting genes including 16 genes encoding chitinase had the elevated abundances in microbial genera. Moreover, NYJ negatively interacted with one bacterial genus and positively interacted with 3 microbial genera under NaHCO3 stress. As a result, 25 bacterial genera were distributed in connectors, and microbial interaction network in rhizospheric soil was different from the one caused by NaHCO3 stress alone.Conclusions: 669 NaHCO3-tolerant species and 208 NaHCO3-tolerant genera of microbes were identified. NYJ application affected microbial community structure and interaction network in rhizospheric soil, increased the abundances of genes related to symbiont, immune, and plant growth-promoting traits in microbes, and activated soil enzymes, thereby mitigating NaHCO3 stress in cucumber.1 These authors contributed equally to this work.

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Biodegradation of crude oil by a defined co-culture of indigenous bacterial consortium and exogenous Bacillus subtilis

Cited by 119 publications

References 33 publications

Enhanced crude oil depletion by constructed bacterial consortium comprising bioemulsifier producer and petroleum hydrocarbon degraders

Enhanced crude oil depletion by constructed bacterial consortium comprising bioemulsifier producer and petroleum hydrocarbon degraders

Petroleum Depletion Property and Microbial Community Shift After Bioremediation Using Bacillus halotolerans T-04 and Bacillus cereus 1-1

Metagenomic analysis of cucumber rhizospheric microbiome provides insights into NaHCO3 stress mitigation by Rheinheimera pacifica NYJ

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