Biodegradation of crude oil and pure hydrocarbons by extreme halophilic archaea from hypersaline coasts of the Arabian Gulf

Al-Mailem, Dina M.; Sorkhoh, N.A.; Al-Awadhi, H.; Eliyas, M.; Radwan, S. S.

doi:10.1007/s00792-010-0312-9

Cited by 145 publications

(53 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, strain MSNC14 also degraded phenanthrene. Three extremely halophilic archaeal strains, Haloferax, Halobacterium and Halococcus isolated on the basis of crude oil utilization also degraded n-alkanes and mono and polyaromatic compounds as the sole sources of carbon and energy [111]. Overall, studies reveal that both bacteria and archaea have the capacity to metabolize n-alkanes with varying chain lengths ( Table 2).…”

Section: B-oxidationmentioning

confidence: 97%

“…Degradation of benzene was also reported in archaea. For example, the crude oil degrading Haloferax, Halobacterium, and Halococcus isolated from a hypersaline Arabian Gulf coast degraded benzene as the sole source of carbon [111].The complete degradation of PAHs requires a community of microorganisms. PAHs are taken up by microorganisms and are activated in aerobic metabolism by insertion of two oxygen atoms by bacteria and green algae to produce either cis-dihydrodiols or phenols [137].…”

Section: Alicyclic Hydrocarbonsmentioning

confidence: 99%

“…Bonfá et al [144] have isolated several strains of Haloferax that degrade a mixture of the PAHs including naphthalene, anthracene, phenanthrene, pyrene and benzo[a] anthracene. Extremely halophilic archaeal strains of Haloferax, Halobacterium, and Halococcus isolated from a hypersaline coastal area of the Arabian Gulf not only degraded crude oil and n-octadecane as the carbon sources, but also grew on phenanthrene [111]. Erdogmu¸s et al [132] showed the degradation of naphthalene, phenanthrene and pyrene as the sole carbon sources by several archaeal strains including Halobacterium piscisalsi, Halorubrum ezzemoulense, Halobacterium salinarium, Haloarcula hispanica, Haloferax sp.…”

Section: Alicyclic Hydrocarbonsmentioning

confidence: 99%

See 2 more Smart Citations

Aerobic Degradation of Petroleum Components by Microbial Consortia

Aa¹,

Essien²

2014

J Pet Environ Biotechnol

View full text Add to dashboard Cite

This article is a state-of-the-art review on the aerobic degradation of petroleum components that are commonly found in the environment. Numerous microorganisms have been isolated and their phylogeny and metabolic capacity to degrade a variety of aliphatic and aromatic hydrocarbons have been demonstrated. This review focuses on recent progress on how microbes degrade hydrocarbons and heteroaromatic components of petroleum contaminants directed towards better understanding of the aerobic degradation processes and their exploitation for bioremediation. The phylogenetic diversity of the oil-degrading microbes was also discussed.

show abstract

Section: B-oxidationmentioning

confidence: 97%

Section: Alicyclic Hydrocarbonsmentioning

confidence: 99%

Section: Alicyclic Hydrocarbonsmentioning

confidence: 99%

See 1 more Smart Citation

Aerobic Degradation of Petroleum Components by Microbial Consortia

Aa¹,

Essien²

2014

J Pet Environ Biotechnol

View full text Add to dashboard Cite

show abstract

“…Studies designed to evaluate the genetic, regulatory, and mechanistic underpinnings of the cellular response to individual salinity factors have laid the foundation for understanding the basic science of prokaryotic osmotolerance (8)(9)(10)(11)(12) but are limited in their ability to capture the relative importance of salinity as a multidimensional perturbation for cell fitness and physiology. A few notable exceptions have explored the combinatorial effects of two salts or salt and other environmental effects on halophilic prokaryotes.…”

mentioning

confidence: 99%

Model Organisms Retain an “Ecological Memory” of Complex Ecologically Relevant Environmental Variation

Beer

Wurtmann

Pinel

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

bAlthough tractable model organisms are essential to characterize the molecular mechanisms of evolution and adaptation, the ecological relevance of their behavior is not always clear because certain traits are easily lost during long-term laboratory culturing. Here, we demonstrate that despite their long tenure in the laboratory, model organisms retain "ecological memory" of complex environmental changes. We have discovered that Halobacterium salinarum NRC-1, a halophilic archaeon that dominates microbial communities in a dynamically changing hypersaline environment, simultaneously optimizes fitness to total salinity, NaCl concentration, and the [K]/[Mg] ratio. Despite being maintained under controlled conditions over the last 50 years, peaks in the three-dimensional fitness landscape occur in salinity and ionic compositions that are not replicated in laboratory culturing but are routinely observed in the natural hypersaline environment of this organism. Intriguingly, adaptation to variations in ion composition was associated with differential regulation of anaerobic metabolism genes, suggesting an intertwined relationship between responses to oxygen and salinity. Our results suggest that the ecological memory of complex environmental variations is imprinted in the networks for coordinating multiple cellular processes. These coordination networks are also essential for dealing with changes in other physicochemically linked factors present during routine laboratory culturing and, hence, retained in model organisms.

show abstract

“…Among bacteria, members of the genera Halomonas (15,16), Marinobacter (6,17), Bacillus (33,46,49), Rhodococcus (46), Pseudomonas (46), Alcaligenes (5), Chromohalobacter (23), Planococcus (29), Streptomyces (25), Arthrobacter (46), and Actinomyces (3) have been shown to degrade hydrocarbons at high salinity. Also, examples of pure cultures of archaea, such as Haloferax (2,10,54), Haloarcula (11,54), Halococcus, and many species of Halobacterium (2,24,32) that have been characterized only phenotypically, were shown to degrade hydrocarbons at high salinity. In addition, a few fungi also were shown to possess the ability to degrade hydrocarbons under hypersaline conditions (39).…”

mentioning

confidence: 99%

Proteogenomic Elucidation of the Initial Steps in the Benzene Degradation Pathway of a Novel Halophile, Arhodomonas sp. Strain Rozel, Isolated from a Hypersaline Environment

Dalvi

Azetsu

Patrauchan

et al. 2012

Appl Environ Microbiol

View full text Add to dashboard Cite

bLately, there has been a special interest in understanding the role of halophilic and halotolerant organisms for their ability to degrade hydrocarbons. The focus of this study was to investigate the genes and enzymes involved in the initial steps of the benzene degradation pathway in halophiles. The extremely halophilic bacteria Arhodomonas sp. strain Seminole and Arhodomonas sp. strain Rozel, which degrade benzene and toluene as the sole carbon source at high salinity (0.5 to 4 M NaCl), were isolated from enrichments developed from contaminated hypersaline environments. To obtain insights into the physiology of this novel group of organisms, a draft genome sequence of the Seminole strain was obtained. A cluster of 13 genes predicted to be functional in the hydrocarbon degradation pathway was identified from the sequence. Two-dimensional (2D) gel electrophoresis and liquid chromatography-mass spectrometry were used to corroborate the role of the predicted open reading frames (ORFs). ORFs 1080 and 1082 were identified as components of a multicomponent phenol hydroxylase complex, and ORF 1086 was identified as catechol 2,3-dioxygenase (2,3-CAT). Based on this analysis, it was hypothesized that benzene is converted to phenol and then to catechol by phenol hydroxylase components. The resulting catechol undergoes ring cleavage via the meta pathway by 2,3-CAT to form 2-hydroxymuconic semialdehyde, which enters the tricarboxylic acid cycle. To substantiate these findings, the Rozel strain was grown on deuterated benzene, and gas chromatography-mass spectrometry detected deuterated phenol as the initial intermediate of benzene degradation. These studies establish the initial steps of the benzene degradation pathway in halophiles. Many hypersaline environments, such as natural saline lakes, salt flats, solar salterns, saline industrial effluents, oil fields, and salt marshes, have been shown to be contaminated with high levels of petroleum hydrocarbons. Among these, oil fields pose a special problem due to their sheer numbers worldwide and due to their high salinity caused by produced water (salty brackish water) generated during oil and natural gas extraction. Produced water is highly saline and contains a complex mixture of hydrocarbons, including alkanes (linear or branched), cycloalkanes, mono-and polyaromatics, asphaltenes, heavy metals, and resins.The ability of microorganisms to degrade aromatic hydrocarbons in terrestrial and marine environments has been studied extensively under oxic conditions (28,43,44,50,56). On the other hand, little is known about hydrocarbon degradation in hypersaline environments. Bioremediation of polluted hypersaline wastewaters and other environments with nonhalophilic microorganisms is difficult because salt inhibits their growth and the degradation of hydrocarbons (41, 57). Therefore, the cleanup of such environments can only be accomplished by stimulating the growth of indigenous microorganisms capable of degrading petroleum hydrocarbons or through the bioaugmentation of halophilic...

show abstract

Biodegradation of crude oil and pure hydrocarbons by extreme halophilic archaea from hypersaline coasts of the Arabian Gulf

Cited by 145 publications

References 32 publications

Aerobic Degradation of Petroleum Components by Microbial Consortia

Aerobic Degradation of Petroleum Components by Microbial Consortia

Model Organisms Retain an “Ecological Memory” of Complex Ecologically Relevant Environmental Variation

Proteogenomic Elucidation of the Initial Steps in the Benzene Degradation Pathway of a Novel Halophile, Arhodomonas sp. Strain Rozel, Isolated from a Hypersaline Environment

Contact Info

Product

Resources

About