Biodegradation of Chlorinated Compounds by White Rot Fungi

Field, James A.

doi:10.1007/0-306-48011-5_6

Cited by 2 publications

(4 citation statements)

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“…However, similar to the investigated enzymatic TCP dechlorination, an oxidative para dechlorination is the first step in TeCP and PCP dechlorination by white rot fungi . These fungi are thought to be the predominant degradation mediators at field sites . Therefore, it is possible to use the determined AKIE Cl of 1.0022 to estimate degrees of PCP and TeCP biodegradation from the Rayleigh equation (eq ).…”

Section: Resultsmentioning

confidence: 91%

“…36 These fungi are thought to be the predominant degradation mediators at field sites. 9 Therefore, it is possible to use the determined AKIE Cl of 1.0022 to estimate degrees of PCP and TeCP biodegradation from the Rayleigh equation (eq 2).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…8 Aerobic microbial degradation is thought to be important for contaminated sawmill sites, and many species of the white rot basidiomycetous fungi are able to degrade CPs. 9 The degradation pathway involves peroxidase and oxidase enzymes (lignin peroxidase, Mn peroxidase, laccase) that are excreted by the fungi in order to degrade lignin. Thereby, these enzymes also oxidatively dechlorinate CPs in the para position, leading to a less chlorinated and less toxic para benzoquinone product.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Abiotic dechlorination (such as photooxidation of CPs in surface water) as well as aerobic or anaerobic microbial degradation are known . Aerobic microbial degradation is thought to be important for contaminated sawmill sites, and many species of the white rot basidiomycetous fungi are able to degrade CPs . The degradation pathway involves peroxidase and oxidase enzymes (lignin peroxidase, Mn peroxidase, laccase) that are excreted by the fungi in order to degrade lignin.…”

Section: Introductionmentioning

confidence: 99%

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Use of Cl and C Isotopic Fractionation to Identify Degradation and Sources of Polychlorinated Phenols: Mechanistic Study and Field Application

Aeppli

Tysklind

Holmstrand

et al. 2012

Environ. Sci. Technol.

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The widespread use of chlorinated phenols (CPs) as a wood preservative has led to numerous contaminated sawmill sites. However, it remains challenging to assess the extent of in situ degradation of CPs. We evaluated the use of compound-specific chlorine and carbon isotope analysis (Cl- and C-CSIA) to assess CP biotransformation. In a laboratory system, we measured isotopic fractionation during oxidative 2,4,6-trichlorophenol dechlorination by representative soil enzymes (C. fumago chloroperoxidase, horseradish peroxidase, and laccase from T. versicolor). Using a mathematical model, the validity of the Rayleigh approach to evaluate apparent kinetic isotope effects (AKIE) was confirmed. A small but significant Cl-AKIE of 1.0022 ± 0.0006 was observed for all three enzymes, consistent with a reaction pathway via a cationic radical species. For carbon, a slight inverse isotope effect was observed (C-AKIE = 0.9945 ± 0.0019). This fractionation behavior is clearly distinguishable from reported reductive dechlorination mechanisms. Based on these results we then assessed degradation and apportioned different types of technical CP mixtures used at two former sawmill sites. To our knowledge, this is the first study that makes use of two-element CSIA to study sources and transformation of CPs in the environment.

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Section: Resultsmentioning

confidence: 91%

Section: ■ Results and Discussionmentioning

confidence: 99%