Biodegradation of chitinous substances and chitinase production by the soil actinomycete Streptomyces rimosus

Characterization of extracellular chitinase from Chitinibacter sp. GC72 and its application in GlcNAc production from crayfish shell enzymatic degradation

“…These results were completely different from that reported in previous studies [28,37,[40][41][42], in which an incomplete conversion of chitin into its monomer was observed during the degradation process. Thus, based on the patterns of hydrolysate formation, it can be concluded that the chitinase obtained from Chitinibacter sp.…”

“…A total of 1% (w/v) colloidal chitin, prewashed with deionized water, was added to the solution at a ratio of 1:2 and incubated for 1 h. Then, the supernatant was discarded after centrifugation at 3500 × g for 5 min. The pellet containing the bound protein was eluted with 50 mmol/L sodium phosphate buffer (pH 7.0) and 1 M NaCl for 5 min according to the modified method proposed by Brzezinska [28]. After repeating the experiment three times, the solution was dialyzed overnight with deionized water and concentrated using ultrafiltration tube.…”

RETRACTED: Purification and characterisation of an acidic and antifungal chitinase produced by a Streptomyces sp.

“…Chitin is a high molecular weight complex polymer and is generally insoluble in aqueous media and hence its uptake as such is not possible. Hence bacteria, especially Streptomyces (Brzezinska et al, 2013;Margino et al, 2010;Nagpure and Gupta, 2013;Prakash et al, 2013;Pradeep et al, 2014) and Bacillus (Kudan and Pichyangkura, 2009;Liu et al, 2010;Natsir et al, 2010;Waghmare and Ghosh, 2010;Dai et al, 2011) species, produce extracellular chitinases for nutritional purposes, in that they are able to utilise chitin as their sole source of carbon. Application of chemical based pesticides causes long-lasting adverse effects on ecosystems and human health.…”

Identification of Novel Chitinolytic Streptomyces. spp from a Sacred Grove and it’s in vitro Antagonistic Activity Analysis

“…Streptomyces sp.PTK19 has reported to have 31.26U/ml with optimum pH 5.5 and 40 o C with a stability at 30-45 o C and has shown antagonestic activity against Fusarium oxysporum PTK2 (Thiagarajan et al, 2011). The nucleotide sequence from Streptomyces rimosus, the amplified gene fragment proved to be similar to the sequence of chitinase-encoding genes of the glycoside hydrolases family 18 (Brzezinska et al, 2013). There were similar reports related to family 19 chitinase identified using Southern hybridization with DIGlabeled chitinase probe showed that fragments around 23kb, 10kb, 5kb and 3kb in a partially digested chromosomal DNA of BRI 13 with BamHI harbored the chitinase gene.…”

“…Chitinolytic systems of Streptomyces are usually composed of a diversity of enzymes with different specificities and chitinase from microorganisms are extremely important for the degradation and recycling of the carbon and nitrogen. Streptomyces rimosus identified based on 16SrRNA and chitinase has a molecular mass of 63kDa active at pH-7 at 40-45 o C, which inhibited the growth of phytopathogen Fusarium solani and Alternaria alternate (Brzezinska et al, 2013). Streptomyces sp.PTK19 has reported to have 31.26U/ml with optimum pH 5.5 and 40 o C with a stability at 30-45 o C and has shown antagonestic activity against Fusarium oxysporum PTK2 (Thiagarajan et al, 2011).…”