Biodegradation of cefdinir by a novel yeast strain, Ustilago sp. SMN03 isolated from pharmaceutical wastewater

Selvi, A.; Salam, Jaseetha Abdul; Das, Nilanjana

doi:10.1007/s11274-014-1710-4

Cited by 30 publications

(22 citation statements)

References 23 publications

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“…The results of LC-MS analysis of pure cefdinir (control) and its degradation products are shown in Figure 7 SMN03, where this phenomenal step was observed only on day 4 [30]. This is considered tobe one of the vital steps in the antibiotic degradation process which was also supported by other researchers [19,43,53,54].…”

Section: Liquid Chromatography Tandem Mass Chromatography (Lcms) Is Asupporting

confidence: 67%

“…SMN04 at an initial concentrationof 250 mg L -1 cefdinir. The kinetic rate constant (K) and half-lives (T1/2) and APE% at optimal cefdinir concentration are presented in [30].Therefore, the calculated half-life reported in this study was found to be the shortest compared to the earlier reports.…”

Section: Degradation Kinetics Of Candida Sp Smn04mentioning

confidence: 47%

“…Pseudomonas putida and Pseudomonas fluorescens [27], Bacillus and Bacteriods [28], mixed bacterial population [29] for A c c e p t e d M a n u s c r i p t 4 degradation of cephalosporin derivatives. Reports are scanty on the use of yeast as degrader of cefdinir, cephalosporin derivative [30]. Among the microorganisms, yeast cells offer several advantages like inexpensive, readily available source of biomass, grow rapidly like bacteria and filamentous fungi.…”

Section: Introductionmentioning

confidence: 99%

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Potentiality of yeastCandidasp. SMN04 for degradation of cefdinir, a cephalosporin antibiotic: kinetics, enzyme analysis and biodegradation pathway

Selvi

Das

2015

Environmental Technology

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A new yeast strain isolated from the pharmaceutical wastewater was capable of utilizing cefdinir as a sole carbon source for their growth in mineral medium. The yeast was identified and named as Candida sp. SMN04 based on morphology and 18S-ITS-D1/D2/D3 rRNA sequence analysis. The interaction between factors pH (3.0-9.0), inoculum dosage (1-7%), time (1-11 day) and cefdinir concentration (50-450 mg/L) was studied using a Box-Behnken design. The factors were studied as a result of their effect on cell dry weight (R1; g/L), extended spectrum β-lactamase (ESBL) assay (R2; mm), P450 activity (R3; U/mL) and degradation (R4; %). Maximum values of R1, R2, R3 and R4 were obtained at central values of all the parameters. The isolated yeast strain efficiently degraded 84% of 250 mg L⁻¹ of cefdinir within 6 days with a half-life of 2.97 days and degradation rate constant of 0.2335 per day. Pseudo-first-order model efficiently described the process. Among the various enzymes tested, the order of activity at the end of Day 4 was noted to be: cytochrome P450 (1.76 ± 0.03) > NADPH reductase (1.51 ± 0.20) > manganese peroxidase and amylase (0.66 ± 0.15; 0.66 ± 0.70). Intermediates were successfully characterized by liquid chromatography-mass spectrometry. The opening of the β-lactam ring involving ESBL activity was considered as one of the major steps in the cefdinir degradation process. Fourier transform-infrared spectroscopy analysis showed the absence of spectral vibrations between 1766 and 1519 cm⁻¹ confirming the complete removal of lactam ring during cefdinir degradation. The results of the present study are promising for the use of isolated yeast Candida sp. SMN04 as a potential bioremediation agent.

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Section: Liquid Chromatography Tandem Mass Chromatography (Lcms) Is Asupporting

confidence: 67%

Section: Degradation Kinetics Of Candida Sp Smn04mentioning

confidence: 47%

Section: Introductionmentioning

confidence: 99%

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Potentiality of yeastCandidasp. SMN04 for degradation of cefdinir, a cephalosporin antibiotic: kinetics, enzyme analysis and biodegradation pathway

Selvi

Das

2015

Environmental Technology

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“…Selvi et al . isolated a kind of fungi named Trametesversicolor which could remove ciprofloxacin and norfloxacin more than 90% 8 . Islas-Espinoza et al .…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of gentamicin by bacterial consortia AMQD4 in synthetic medium and raw gentamicin sewage

Liu

Chen

et al. 2017

Sci Rep

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Gentamicin, a broad spectrum antibiotic of the aminoglycoside class, is widely used for disease prevention of human beings as well as animals. Nowadays the environmental issue caused by the disposal of wastes containing gentamicin attracts increasing attention. In this study, a gentamicin degrading bacterial consortia named AMQD4, including Providencia vermicola, Brevundimonas diminuta, Alcaligenes sp. and Acinetobacter, was isolated from biosolids produced during gentamicin production for the removal of gentamicin in the environment. The component and structure of gentamicin have a great influence on its degradation and gentamicin C1a and gentamicin C2a were more prone to being degraded. AMQD4 could maintain relatively high gentamicin removal efficiency under a wide range of pH, especially in an alkaline condition. In addition, AMQD4 could remove 56.8% and 47.7% of gentamicin in unsterilized and sterilized sewage in a lab-scale experiment, respectively. And among the isolates in AMQD4, Brevundimonas diminuta BZC3 performed the highest gentamicin degradation about 50%. It was speculated that aac3iia was the gentamicin degradation gene and the main degradation product was 3′-acetylgentamicin. Our results suggest that AMQD4 and Brevundimonas diminuta BZC3 could be important candidates to the list of superior microbes for bioremediation of antibiotic pollution.

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“…SMN03, and Candida sp. SMN04 toward cefdinir biodegradation have been reported in our previous studies .…”

Section: Introductionmentioning

confidence: 55%

Degradation of cefdinir by Candida Sp. SMN04 and MgO nanoparticles—An integrated (Nano‐Bio) approach

Selvi

Das

2015

Env Prog and Sustain Energy

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The present study evaluates the effect of integrated nano‐bio approach involving nanoscale magnesium oxide (n‐MgO) and yeast Candida sp. SMN04 on cefdinir degradation in aqueous medium. The nanoparticle was chemically synthesized and characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM), EDAX analysis, and particle size analyser. Nano‐bio integrated system was prepared using chemically synthesized n‐MgO (10 mg mL−1), coated onto the surface of yeast cells without causing any lethal effects to the cell. Cefdinir (250 mg L−1) degradation was studied using individual and nano‐bio integrated system both. Nano‐bio integrated system was found to be more effective for cefdinir degradation compared to native yeast cell. The adherence of nanoparticles on the surface of the yeast cells increased the permeability of the cell membrane, thereby enhancing the entry of cefdinir into the cell. The kinetic data showed the half‐life of cefdinir, which was 1.46 days for integrated system and 2.97 days for native yeast, which confirmed that the integrated approach reduced the half‐life to less than half of the time taken by the yeast alone. This study signifies the potential efficacy of the nano‐bio integrated approach to serve as an effective remedial tool for the treatment of pharmaceutical wastewater. © 2015 American Institute of Chemical Engineers Environ Prog, 35: 706–714, 2016

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Biodegradation of cefdinir by a novel yeast strain, Ustilago sp. SMN03 isolated from pharmaceutical wastewater

Cited by 30 publications

References 23 publications

Potentiality of yeastCandidasp. SMN04 for degradation of cefdinir, a cephalosporin antibiotic: kinetics, enzyme analysis and biodegradation pathway

Potentiality of yeastCandidasp. SMN04 for degradation of cefdinir, a cephalosporin antibiotic: kinetics, enzyme analysis and biodegradation pathway

Biodegradation of gentamicin by bacterial consortia AMQD4 in synthetic medium and raw gentamicin sewage

Degradation of cefdinir by Candida Sp. SMN04 and MgO nanoparticles—An integrated (Nano‐Bio) approach

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