Biodegradation of asphaltene and petroleum compounds by a highly potent Daedaleopsis sp.

Abstract

Petroleum, as the major energy source, is indispensable from our lives. Presence of compounds resistant to degradation can pose risks for human health and environment. Basidiomycetes have been considered as powerful candidates in biodegradation of petroleum compounds via secreting ligninolytic enzymes. In this study a wood-decaying fungus was isolated by significant degradation ability that was identified as Daedaleopsis sp. by morphological and molecular identification methods. According to GC/MS studies, incubation of heavy crude oil with Daedaleopsis sp. resulted in increased amounts of <C24 hydrocarbons and decreased amounts of >C24 compounds. Degradation of asphaltene, anthracene, and dibenzofuran by the identified fungal strain was determined to evaluate its potential in biodegradation. After 14 days of incubation, Daedaleopsis sp. could degrade 93.7% and 91.2% of anthracene and dibenzofuran, respectively, in pH 5 and 40 °C in optimized medium, as revealed by GC/FID. Notably, analysis of saturates, aromatics, resins, and asphaltenes showed a reduction of 88.7% and 38% in asphaletene and aromatic fractions. Laccase, lignin peroxidase, and manganese peroxidase activities were enhanced from 51.3, 145.2, 214.5 U ml in the absence to 121.5, 231.4, and 352.5 U ml in the presence of heavy crude oil, respectively. This is the first report that Daedaleopsis sp. can degrade asphaltene and dibenzofuran. Moreover, compared to the reported results of asphaltene biodegradation, this strain was the most successful. Thus, Daedaleopsis sp. could be a promising candidate for biotransformation of heavy crude oil and biodegradation of recalcitrant toxic compounds.

Bioremediation of soils contaminated with petroleum solid wastes and drill cuttings byPleurotussp. strains under different treatment scales

“…(Yanto and Tachibana 2013) and recently the basidiomycete Daedaleopsis sp. (Pourfakhraei et al 2018). Asphalthenes biodegradation rates achieved by Pleurotus sp.…”

Biodegradation of Crude Oil and Lignin-Modifying Enzyme Activity of White Rot Basidiomycetes

“…Nevertheless, unlike Daedaleopsis sp. [8], supplementation of medium with an easily metabolizable carbon source (glucose) or nitrogen source (yeast extract) in this work did not stimulate the pollutant elimination. Nevertheless, the addition of glucose as well as copper to the growing culture significantly promoted the fungus growth and enzyme production; one can assume that maintaining of a fungal culture enzymatic activity during incubation will favor the bioremediation process.…”

“…Recently, significant efforts were undertaken to screen and identify WRB strains that are most efficient in degrading TPH [5][6][7][8]. A large majority of the bioremediation investigations to date were done with Phaneroachaete chrysosporium as the model microorganism [5,9,10].…”

Bioremediation of soils contaminated with petroleum solid wastes and drill cuttings by Pleurotus sp. under different treatment scales

“…B-7 and B-15 degrade resins fraction considerably more than any other treatment, and all fungal strains degraded the aromatics compounds, contrasting with the laboratory experiment. These results suggest that resin degradation is a consistent process in the strains studied, and again the rates are higher than those for other basidiomycete [23]. Pleurotus sp.…”

“…(77% in 30 days) [22] and recently the basidiomycete Daedaleopsis sp. (88% in 30 days) [23]. Asphaltenes biodegradation rates achieved by Pleurotus sp.…”