Biodegradation of aromatic-aliphatic copolyesters and polyesteramides by esterase activity-producing microorganisms

Novotný, Čeněk; Erbanová, Pavla; Sezimová, Hana; Malachová, Kateřina; Rybková, Zuzana; Malinová, Lenka; Prokopová, Irena; Brožek, Jiřı́

doi:10.1016/j.ibiod.2014.10.010

Cited by 28 publications

(15 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Esterases, lipases and cutinases are hydrolases that are instrumental in plastic degradation (Table 1) (Ruiz et al 1999;Sangale et al 2012;Novotn y et al 2015;Mohan et al 2016). Hydrolases are important for enzymatic polymer cleavage wherein ester bonds are broken through a nucleophilic attack on carbonyl carbon atoms created by previous oxidation reactions (Devi et al 2016).…”

Section: Plastic Degradation By Extracellular and Intracellular Enzymesmentioning

confidence: 99%

“…lipase but only partially degraded by a recombinant esterase from P. fluorescens (Biffinger et al 2015). The production of high amounts of extracellular esterases and lipases in P. aeruginosa was reported to facilitate the degradation of aromatic-aliphatic polyesters and polyesteramides (Novotn y et al 2015). An extracellular cutinase from P. mendocina acting on a PET film with 7% crystallinity caused a 5% weight loss of the film after 96 h and produced terephthalic acid (TPA) and ethylene glycol (EG) as the sole products (Ronkvist et al 2009).…”

Section: Plastic Degradation By Extracellular and Intracellular Enzymesmentioning

confidence: 99%

“…Once inside the cell, the TPA can then be broken down intracellularly into protocatechuate, which undergoes ring cleavage via the aromatic catabolism pathway, before generating TCA cycle intermediates (Fig. 2) (Karegoudar and Pujar 1985;Wang et al 1995;Novotn y et al 2015;Yoshida et al 2016).…”

Section: From Extracellular Degradation To Potential Intracellular Mementioning

confidence: 99%

See 2 more Smart Citations

Degradation and metabolism of synthetic plastics and associated products byPseudomonassp.: capabilities and challenges

2017

View full text Add to dashboard Cite

SummarySynthetic plastics, which are widely present in materials of everyday use, are ubiquitous and slowly-degrading polymers in environmental wastes. Of special interest are the capabilities of microorganisms to accelerate their degradation. Members of the metabolically diverse genus Pseudomonas are of particular interest due to their capabilities to degrade and metabolize synthetic plastics. Pseudomonas species isolated from environmental matrices have been identified to degrade polyethylene, polypropylene, polyvinyl chloride, polystyrene, polyurethane, polyethylene terephthalate, polyethylene succinate, polyethylene glycol and polyvinyl alcohol at varying degrees of efficiency. Here, we present a review of the current knowledge on the factors that control the ability of Pseudomonas sp. to process these different plastic polymers and their by-products. These factors include cell surface attachment within biofilms, catalytic enzymes involved in oxidation or hydrolysis of the plastic polymer, metabolic pathways responsible for uptake and assimilation of plastic fragments and chemical factors that are advantageous or inhibitory to the biodegradation process. We also highlight future research directions required in order to harness fully the capabilities of Pseudomonas sp. in bioremediation strategies towards eliminating plastic wastes.

show abstract

Section: Plastic Degradation By Extracellular and Intracellular Enzymesmentioning

confidence: 99%

Section: Plastic Degradation By Extracellular and Intracellular Enzymesmentioning

confidence: 99%

See 1 more Smart Citation

Degradation and metabolism of synthetic plastics and associated products byPseudomonassp.: capabilities and challenges

2017

View full text Add to dashboard Cite

show abstract

“…Poly(1,4-butylene adipate-co-terephthalate) (PBAT) was designed as a co-aliphatic-aromatic polyester that maintained some of the beneficial properties of aromatic polyesters while being easier to biodegrade due to its aliphatic components. Microbial biodegradation of PBAT (Kijchavengkul et al 2010 ; Nakajima-Kambe et al 2009 ; Trinh Tan et al 2008 ; Witt et al 2001 ) and other aromatic-aliphatic co-polyesters like poly(ethylene terephthalate)/poly(lactic acid) (Hu et al 2008 ; Novotny et al 2015 ) has been shown with organisms including species of Pseudomonas (Cerda-Cuellar et al 2004 ; Novotny et al 2015 ). However, few of the enzymes with the ability to hydrolyse these aromatic-aliphatic co-polyesters were identified or characterised.…”

Section: Introductionmentioning

confidence: 99%

PpEst is a novel PBAT degrading polyesterase identified by proteomic screening of Pseudomonas pseudoalcaligenes

Wallace

Haernvall

Ribitsch

et al. 2016

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

A novel esterase, PpEst, that hydrolyses the co-aromatic-aliphatic polyester poly(1,4-butylene adipate-co-terephthalate) (PBAT) was identified by proteomic screening of the Pseudomonas pseudoalcaligenes secretome. PpEst was induced by the presence of PBAT in the growth media and had predicted arylesterase (EC 3.1.1.2) activity. PpEst showed polyesterase activity on both whole and milled PBAT film releasing terephthalic acid and 4-(4-hydroxybutoxycarbonyl)benzoic acid while end product inhibition by 4-(4-hydroxybutoxycarbonyl)benzoic acid was observed. Modelling of an aromatic polyester mimicking oligomer into the PpEst active site indicated that the binding pocket could be big enough to accommodate large polymers. This is the first report of a PBAT degrading enzyme being identified by proteomic screening and shows that this approach can contribute to the discovery of new polymer hydrolysing enzymes. Moreover, these results indicate that arylesterases could be an interesting enzyme class for identifications of polyesterases.Electronic supplementary materialThe online version of this article (doi:10.1007/s00253-016-7992-8) contains supplementary material, which is available to authorized users.

show abstract

“…Recently, environmentally friendly food packaging has received increased attention owing to global environmental concerns. Biobased products are the representative environmentally friendly packaging materials and can be produced from renewable resources by microorganisms and plants [69][70][71]. To enhance the production of bio-based products from microorganisms and plants, metabolic engineering is actively used [72].…”

Section: Food Packagingmentioning

confidence: 99%

Food metabolomics: from farm to human

Kim

Yun

et al. 2016

Current Opinion in Biotechnology

106

View full text Add to dashboard Cite

Biodegradation of aromatic-aliphatic copolyesters and polyesteramides by esterase activity-producing microorganisms

Cited by 28 publications

References 32 publications

Degradation and metabolism of synthetic plastics and associated products byPseudomonassp.: capabilities and challenges

Degradation and metabolism of synthetic plastics and associated products byPseudomonassp.: capabilities and challenges

PpEst is a novel PBAT degrading polyesterase identified by proteomic screening of Pseudomonas pseudoalcaligenes

Food metabolomics: from farm to human

Contact Info

Product

Resources

About