Biodegradation of anthracene by a newly isolated bacterial strain,<i>Bacillus thuringiensis</i>AT.ISM.1, isolated from a fly ash deposition site

Tarafdar, Abhrajyoti; Sinha, Alok; Masto, Reginald Ebhin

doi:10.1111/lam.12785

Cited by 39 publications

(17 citation statements)

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“…GC-MS analysis has been widely used in many previous studies to identify the byproducts and metabolic intermediates produced during different anthracene biodegradation pathways by various bacterial species [17,33,59,60]. Therefore, it was selected in the current study to detect the different metabolites produced during the anthracene biodegradation and to suggest the proposed anthracene biodegradation pathways followed by the selected strains.…”

Section: Discussionmentioning

confidence: 99%

“…For example, Bacillus thuringiensis AT.ISM.1 degraded anthracene through anthraquinone, phthalic acid derivatives, benzoic acid derivatives, and pyrocatechol and benzaldehyde [17]. Also, another study showed that Polyporus sp.…”

Section: Discussionmentioning

confidence: 99%

“…Anthracene as a type of PAHs is widely distributed in the environments due to many anthropogenic activities, like oil refining and the petro-chemical industry [12]. Exposure to high doses of anthracene has toxic effects on skin and tissues [17].…”

Section: Introductionmentioning

confidence: 99%

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Different metabolic pathways involved in anthracene biodegradation by Brevibacillus, Pseudomonas and Methylocystis Species

Magdy

Gaber

Sebak

et al. 2022

Beni-Suef Univ J Basic Appl Sci

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Background Polycyclic aromatic hydrocarbons (PAHs) such as anthracene are one of the most toxic contaminants to our environment. Microbial biodegradation of these xenobiotics is a cost-effective technological solution. The present study aimed to recover some bacterial isolates from Beni-Suef Governorate in Egypt with high capabilities of anthracene biodegradation. The selected isolates were molecularly characterized by 16S rRNA gene sequencing, the degree of anthracene biodegradation was monitored using optical density (OD) and high-performance liquid chromatography (HPLC), PCR amplification of some selected genes encoding biodegradation of PAHs was monitored, and gas chromatography–mass spectrometry (GC–MS) analysis was applied for detecting the resulted metabolites. Result Three bacterial isolates were studied, the 16s rRNA sequences of the isolates showed homology of the first isolate to Brevibacillus sp. (94.58 %), the second isolates showed homology to Pseudomonas sp. (94.53%) and the third isolate showed homology to Methylocystis sp. (99.61 %), all isolates showed the ability to degrade anthracene. PCR amplification of some selected genes encoding biodegradation of PAHs revealed the presence of many biodegrading genes in the selected strains. Gas chromatography-mass spectrometry (GC–MS) analysis of the metabolites resulted from anthracene biodegradation in the present study suggested that more than one biodegradation pathway was followed by the selected isolates. Conclusions The selected strains could represent a potential bioremediation tool in solving the PAHs problem in the Egyptian environment with a clean and cost-effective technique. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Different metabolic pathways involved in anthracene biodegradation by Brevibacillus, Pseudomonas and Methylocystis Species

Magdy

Gaber

Sebak

et al. 2022

Beni-Suef Univ J Basic Appl Sci

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“…was favorable when considering the degradation (%) achieved using other microorganisms reported in the literature. Numerous fungal species, such as Mycobacterium sp., were reported to degrade PAHs [35]. The transformation of PAHs using fungal species may involve several enzymatic pathways [35].…”

Section: Removal Of Polycyclic Aromatic Hydrocarbons In Test Systemsmentioning

confidence: 99%

“…Numerous fungal species, such as Mycobacterium sp., were reported to degrade PAHs [35]. The transformation of PAHs using fungal species may involve several enzymatic pathways [35]. The degradation (%) of PAHs from media containing pyrene and phenanthrene by Mycobacterium sp.…”

Section: Removal Of Polycyclic Aromatic Hydrocarbons In Test Systemsmentioning

confidence: 99%

Bioremediation of Polycyclic Aromatic Hydrocarbons from Industry Contaminated Soil Using Indigenous Bacillus spp.

et al. 2021

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Polycyclic aromatic hydrocarbons (PAHs) are reportedly toxic, ubiquitous and organic compounds that can persist in the environment and are released largely due to the incomplete combustion of fossil fuel. There is a range of microorganisms that are capable of degrading low molecular weight PAHs, such as naphthalene; however, fewer were reported to degrade higher molecular weight PAHs. Bacillus spp. has shown to be effective in neutralizing polluted streams containing hydrocarbons. Following the growing regulatory requirement to meet the PAH specification upon disposal of contaminated soil, the following study aimed to identify potential Bacillus strains that could effectively remediate low and high molecular weight PAHs from the soil. Six potential hydrocarbon-degrading strains were formulated into two prototypes and tested for the ability to remove PAHs from industry-contaminated soil. Following the dosing of each respective soil system with prototypes 1 and 2, the samples were analyzed for PAH concentration over 11 weeks against an un-augmented control system. After 11 weeks, the control system indicated the presence of naphthalene (3.11 µg·kg−1), phenanthrene (24.47 µg·kg−1), fluoranthene (17.80 µg·kg−1) and pyrene (28.92 µg·kg−1), which illustrated the recalcitrant nature of aromatic hydrocarbons. The soil system dosed with prototype 2 was capable of completely degrading (100%) naphthalene, phenanthrene and pyrene over the experimental period. However, the accumulation of PAHs, namely phenanthrene, fluoranthene and pyrene, were observed using prototype 1. The results showed that prototype 2, consisting of a combination of Bacillus cereus and Bacillus subtilis strains, was more effective in the biodegradation of PAHs and intermediate products. Furthermore, the bio-augmented system dosed with prototype 2 showed an improvement in the overall degradation (10–50%) of PAHs, naphthalene, phenanthrene and pyrene, over the un-augmented control system. The following study demonstrates the potential of using Bacillus spp. in a bioremediation solution for sites contaminated with PAHs and informs the use of biological additives for large-scale environmental remediation.

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Green titanium dioxide (TiO₂) nanoparticles assisted biodegradation of anthracene employing Serratia quinivorans HP5

Chakravarty,

Deka,

Chowdhury

2024

J Basic Microbiol

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The anthracene biodegradation potential of Serratia quinivorans HP5 was studied under a controlled laboratory environment. The green TiO2 nanoparticles (NPs) synthesized from Paenibacillus sp. HD1PAH was used to accelerate the biodegradation process. The synergistic application of TiO2 NPs and S. quinivorans HP5 resulted in a reduction of anthracene concentration by 1.2 folds in liquid‐medium and 1.5 folds in contaminated soil. Gas‐chromatography and mass‐spectrometric investigation showed the production of four anthracene derivatives, namely 1,2‐anthracene dihydrodiol, 6,7‐benzocoumarin, anthrone, and 9,10‐anthraquinoneat the termination of experimental periods. Furthermore, bacterial biomass increased by 23.3 folds in the presence of TiO2 NPs, and overall soil enzyme activities were enhanced by 4.2 folds in the treated samples. In addition, there was a negative correlation observed between the biomass of S. quinivorans HP5 and the concentrations of anthracene, suggesting the involvement of bacterium in anthracene biodegradation processes. The degradation pathway of anthracene revealed its transformation into the less toxic compound 9,10‐anthraquinone. Overall, this study elucidates a novel biodegradation pathway for anthracene and highlights the potential of nano‐assisted bacterial remediation as a promising approach for environmental cleanup.

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Biodegradation of anthracene by a newly isolated bacterial strain,Bacillus thuringiensisAT.ISM.1, isolated from a fly ash deposition site

Cited by 39 publications

References 38 publications

Different metabolic pathways involved in anthracene biodegradation by Brevibacillus, Pseudomonas and Methylocystis Species

Different metabolic pathways involved in anthracene biodegradation by Brevibacillus, Pseudomonas and Methylocystis Species

Bioremediation of Polycyclic Aromatic Hydrocarbons from Industry Contaminated Soil Using Indigenous Bacillus spp.

Green titanium dioxide (TiO₂) nanoparticles assisted biodegradation of anthracene employing Serratia quinivorans HP5

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Biodegradation of anthracene by a newly isolated bacterial strain,Bacillus thuringiensisAT.ISM.1, isolated from a fly ash deposition site

Cited by 39 publications

References 38 publications

Different metabolic pathways involved in anthracene biodegradation by Brevibacillus, Pseudomonas and Methylocystis Species

Different metabolic pathways involved in anthracene biodegradation by Brevibacillus, Pseudomonas and Methylocystis Species

Bioremediation of Polycyclic Aromatic Hydrocarbons from Industry Contaminated Soil Using Indigenous Bacillus spp.

Green titanium dioxide (TiO2) nanoparticles assisted biodegradation of anthracene employing Serratia quinivorans HP5

Contact Info

Product

Resources

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Green titanium dioxide (TiO₂) nanoparticles assisted biodegradation of anthracene employing Serratia quinivorans HP5