Biodegradation of Aliphatic and Aromatic Fractions of Heavy Crude Oil–Contaminated Soil: A Pilot Study

Minai-Tehrani, Dariush; Herfatmanesh, Ali

doi:10.1080/10889860701351589

Cited by 37 publications

(23 citation statements)

References 19 publications

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“…At the end of the experiment (12 months), the amount of TPH in the contaminated soils was determined according to the method used by Minai-Tehrani and Herfatmanesh (2007). From each sample, 1 g of soil was removed and dried at 50°C.…”

Section: Total Petroleum Hydrocarbon (Tph) Extractionmentioning

confidence: 99%

Assessment of bioremediation of aliphatic, aromatic, resin, and asphaltene fractions of oil-sludge-contaminated soil

Minai-Tehrani

Rohanifar

Azami

2014

Int. J. Environ. Sci. Technol.

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Oil sludge is a viscous material consisting of resin, asphaltene, sand, and water, which is usually formed at the bottom of oil reservoir tanks. Asphaltene and resin contents of oil sludge make it more resistant to biodegradation. Disposal of oil sludge is the main problem of oil industry; discharge of oil sludge into the soil causes damage to the environment. Bioremediation is an efficient, cheap, and environmentally friendly method for oil-sludge treatment. The aim of this study was to investigate the biodegradation of oil-sludge fractions in contaminated soil for a period of 12 months. The oil sludge was mixed with soil with the final concentration of 5 % (w/w), and the nutrients such as phosphate and nitrate salts were added to soil. Finally, reduction of aliphatic, aromatic, resin, and asphaltene was tested. The results showed that the reduction of total petroleum hydrocarbon was 31 ± 5 % during 12 months of the treatment. About 60 ± 8 % of total aliphatic fractions, mainly C 14 -C 22 , decreased. Analysis for the detection of two, three, and four rings of polycyclic aromatic hydrocarbons demonstrated about 42 ± 3 % reduction of total aromatic fractions, whereas the resin (6 ± 0.8 %) and asphaltene (4 ± 0.5 %) fractions were slightly biodegraded. In conclusion, biotreatment of oil sludge during 12 months could well reduce aliphatic and aromatic fractions, but more time is needed for resin and asphaltene reduction.

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Section: Total Petroleum Hydrocarbon (Tph) Extractionmentioning

confidence: 99%

Assessment of bioremediation of aliphatic, aromatic, resin, and asphaltene fractions of oil-sludge-contaminated soil

Minai-Tehrani

Rohanifar

Azami

2014

Int. J. Environ. Sci. Technol.

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“…Therefore, these microorganisms are adapted to live with hydrophobic compounds as petroleum products, as the sole carbon source. It has been proved that hydrocarbon addition in soil is followed by an increase in microbial population (Minai-Tehrani and Herfatmanesh 2007), indicating a positive correlation between the oil content and the presence of hydrocarbon-utilizing microflora. In addition, these indigenous microbial populations could be highly adapted to the toxic effects of petroleum or its degradation products (Wesp et al 2000).…”

Section: Resultsmentioning

confidence: 99%

Richness of drilling sludge taken from an oil field quagmire: potentiality and environmental interest

Mansour

Nasrallah

Djenane

et al. 2016

Int. J. Environ. Sci. Technol.

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The drilling sludge represents a complex environment, containing several types of pollutants that can be even used as nutrients by indigenous microorganisms, like hydrocarbon-degrading bacteria, having good potentialities for the biodegradation of petroleum products. In this study, a drilling sludge was collected from drilling quagmire. Physicochemical characterization of the drilling sludge was done. Its mineralogy was obtained by diffractometry. The indigenous aerobic sludge hydrocarbon-degrading bacteria were checked by counting on Bushnell-Haas medium, and their isolation and purification were performed by the selective microbial enrichment technique in a batch-enriched Bushnell-Haas culture, with crude oil as the sole carbon source. Isolates were characterized, and their power to emulsify crude oil was determined by emulsification index and oil spreading tests. Environmental conditions in the quagmire, like temperature, pH and moisture, were suitable for bacterial development. Physicochemical characteristics of the drilling sludge showed richness in chemical elements and promote microbial life. Fifteen different colonies of hydrocarbon-degrading bacteria were isolated and purified; they have diversified morphological and microscopic aspects. Most isolates had a good emulsification index (between 31 and 76 %). Oil spreading test gave clear zone diameters [28 mm, with a maximum of 60 mm. The results of these investigations prove the elementary, mineralogy and microbiology richness of drilling sludge and reveal the high diversity of its indigenous hydrocarbon-degrading bacterial flora. These properties can be exploited for the own restoration of petroleum quagmires in oil fields, by means of bioremediation applications and by integrating indigenous microorganisms.

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“…The collected fraction was then purified by passing through silica G-60. The column was sequentially eluted with hexane to obtain the aliphatic fractions and then with hexane:DCM (1:1) to elute the aromatic fractions [38].…”

Section: Biotransformation Studies Using Gc-msmentioning

confidence: 99%

Microbial-Enhanced Heavy Oil Recovery under Laboratory Conditions by Bacillus firmus BG4 and Bacillus halodurans BG5 Isolated from Heavy Oil Fields

Shibulal

Al-Bahry

Al-Wahaibi

et al. 2018

Colloids and Interfaces

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Microbial Enhanced Oil Recovery (MEOR) is one of the tertiary recovery methods. The high viscosity and low flow characteristics of heavy oil makes it difficult for the extraction from oil reservoirs. Many spore-forming bacteria were isolated from Oman oil fields, which can biotransform heavy crude oil by changing its viscosity by converting heavier components into lighter ones. Two of the isolates, Bacillus firmus BG4 and Bacillus halodurans BG5, which showed maximum growth in higher concentrations of heavy crude oil were selected for the study. Gas chromatography analysis of the heavy crude oil treated with the isolates for nine days showed 81.4% biotransformation for B. firmus and 81.9% for B. halodurans. In both cases, it was found that the aromatic components in the heavy crude oil were utilized by the isolates, converting them to aliphatic species. Core flooding experiments conducted at 50 • C, mimicking reservoir conditions to prove the efficiency of the isolates in MEOR, resulted in 10.4% and 7.7% for B. firmus and B. halodurans, respectively, after the nine-day shut-in period. These investigations demonstrated the potential of B. firmus BG4 and B. halodurans BG5 as an environmentally attractive approach for heavy oil recovery.

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Biodegradation of Aliphatic and Aromatic Fractions of Heavy Crude Oil–Contaminated Soil: A Pilot Study

Cited by 37 publications

References 19 publications

Assessment of bioremediation of aliphatic, aromatic, resin, and asphaltene fractions of oil-sludge-contaminated soil

Assessment of bioremediation of aliphatic, aromatic, resin, and asphaltene fractions of oil-sludge-contaminated soil

Richness of drilling sludge taken from an oil field quagmire: potentiality and environmental interest

Microbial-Enhanced Heavy Oil Recovery under Laboratory Conditions by Bacillus firmus BG4 and Bacillus halodurans BG5 Isolated from Heavy Oil Fields

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